Rubra_00540 : CDS information

Location

Organism	Streptomyces achromogenes var. rubradiris
Strain	NRRL 3061 (=NBRC 14000)
Entry name	Rubradirin
Contig	AJ871581
Start / Stop / Direction	97,072 / 96,035 / - [in whole cluster] 97,072 / 96,035 / - [in contig]
Location	complement(96035..97072) [in whole cluster] complement(96035..97072) [in contig]
Type	CDS
Length	1,038 bp (345 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative glucose-1-phosphate thymidylyltransferase putative dTDP-glucose synthase
Product (GenBank)	putative TDP-glucose synthase
Gene
Gene (GenBank)	rubN1
EC number	2.7.7.24
Keyword	D-rubranitrose
Note
Note (GenBank)
Reference	ACC Q2PC36 PmId [18080113] Biosynthesis of rubradirin as an ansamycin antibiotic from Streptomyces achromogenes var. rubradiris NRRL3061. (Arch Microbiol. , 2008) comment Rubradirin生合成clusterの報告。 Table1>配列比較からの推定機能 RubN1(345aa): dTDP-glucose synthase RubN1-N8はdTDP-D-rubranitroseの合成に推定的に関与していることが配列解析で明らかになった。
Related Reference	ACC Q194R4 NITE Mith_00060 PmId [9150235] Cloning and insertional inactivation of Streptomyces argillaceus genes involved in the earliest steps of biosynthesis of the sugar moieties of the antitumor polyketide mithramycin. (J Bacteriol. , 1997) comment 21st(Q194R4) 50%, 3e-83 Streptomyces argillaceus_mtmD DTDP-glucose synthase E. coli発現タンパクで以下の2酵素の活性みている。 mtmD: glucose-1-phosphate:dTTP thymidylyl transferase mtmE: dTDP-D-glucose 4,6-dehydratase mtmD mutant -- accumulates premithramycinone

Sequence

>putative glucose-1-phosphate thymidylyltransferase [putative TDP-glucose synthase]
MKALVLAGGSGSRLRPLTLSMPKQLIPVANEPVLRHCLRNIRAMGIRQVGVVEGEHGDQI
RAALGDGAALGLDLTYIRQDTPAGLAHCVRIAAGFLGNEDFVLYLGDNVVVGDLAGAAEE
FRSSRPAAKLLLAKVADPSQYGVAGLRADGNVRAVVEKSPCPPSDLAIMGVYFFTPEICA
AVDRISPSPRGELEITDAIQYLIDAGGTVSAQPFTGLWKDTGTVEGLLACNRLLLDGLPP
NIAGVVDAASVVHGDVTVESGARVTRSVLRGPLVLGAGSVVEDSVLGPYVALGPQCRVRK
AVVRDSILLGGSVVRGGPPVTGEISNGRLRVTAPATSSAEDRSAS

>putative glucose-1-phosphate thymidylyltransferase [putative TDP-glucose synthase]
ATGAAGGCACTCGTGCTGGCCGGCGGGAGCGGTAGCCGGTTGCGCCCGTTGACGCTCTCG
ATGCCCAAGCAGCTGATCCCCGTCGCGAACGAGCCGGTCCTGCGGCACTGCTTGCGCAAC
ATCCGGGCCATGGGCATACGGCAGGTCGGCGTCGTGGAGGGCGAGCACGGCGACCAGATC
CGGGCGGCGCTGGGGGACGGGGCGGCGCTGGGCCTGGACCTCACCTACATCCGGCAGGAC
ACCCCGGCCGGGCTCGCGCACTGTGTCCGTATCGCCGCGGGCTTCCTCGGCAACGAGGAT
TTCGTCCTGTACCTGGGTGACAATGTGGTGGTCGGTGATCTGGCGGGCGCGGCCGAGGAG
TTCAGGTCCAGCCGGCCGGCCGCGAAGCTGCTGCTTGCCAAGGTCGCCGATCCGAGCCAG
TACGGTGTGGCCGGCCTCCGTGCGGACGGCAATGTCCGTGCGGTGGTGGAGAAGTCGCCT
TGCCCGCCGAGCGATCTGGCGATCATGGGTGTCTACTTCTTCACGCCGGAGATCTGTGCC
GCAGTAGACCGGATCAGTCCCAGCCCGCGCGGTGAACTGGAGATCACCGACGCGATCCAG
TACCTGATCGATGCGGGGGGTACCGTCTCCGCGCAGCCTTTCACCGGCCTGTGGAAGGAC
ACCGGCACGGTCGAGGGCCTGCTGGCCTGCAATCGGCTGCTGCTGGACGGGTTACCTCCG
AACATCGCCGGTGTGGTCGACGCGGCGAGTGTCGTGCACGGCGATGTGACGGTGGAGAGC
GGTGCGCGGGTGACCCGCTCCGTGCTGCGCGGGCCGCTCGTCCTCGGCGCGGGCAGCGTG
GTCGAGGACAGCGTCCTGGGCCCGTATGTCGCCCTCGGGCCGCAGTGCCGGGTGCGCAAA
GCCGTCGTGCGGGACTCGATTCTCCTGGGCGGCTCCGTCGTGCGGGGAGGGCCACCGGTC
ACCGGTGAGATATCCAACGGCCGGCTGCGGGTCACGGCACCTGCCACCTCCTCGGCCGAG
GACCGCAGTGCGTCGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005835 Nucleotidyl transferase (Domain) PF00483 NTP_transferase IPR005908 Glucose-1-phosphate thymidylyltransferase, long form (Family) TIGR01208 RmlA_long
SignalP	No significant hit
TMHMM	No significant hit

Rubra_00530 Rubra_00550