Adria_00060 : CDS information

Location

Organism	Streptomyces peucetius
Strain	ATCC 29050 (=NBRC 100596)
Entry name	Doxorubicin
Contig	U77891
Start / Stop / Direction	21,134 / 22,651 / + [in whole cluster] 4,129 / 5,646 / + [in contig]
Location	21134..22651 [in whole cluster] 4129..5646 [in contig]
Type	CDS
Length	1,518 bp (505 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative NDP-hexose 2,3-dehydratase
Product (GenBank)	doxorubicin biosynthesis protein DnmT
Gene
Gene (GenBank)	dnmT
EC number
Keyword	daunosamine
Note
Note (GenBank)	catalyzes unknown step in biosynthesis of daunosamine, an intermediate of doxorubicin biosynthesis; deoxysugar biosynthesis enzyme
Reference	ACC P95833 PmId [8955419] Enhanced antibiotic production by manipulation of the Streptomyces peucetius dnrH and dnmT genes involved in doxorubicin (adriamycin) biosynthesis. (J Bacteriol. , 1996) comment dnmT mutantsはRHOが3倍増、daunorubicin (DNR)検出されず。 dnmT(-) strainでdnmTを過剰発現すると、RHO, DNR共にwildレベルに回復。よって、dnmTはdaunosamineの生合成かRHOへの付着に関与するか、この過程の調節に関与するかもしれない。 dnrH mutant strainsでのdnmT過剰発現は、RHOが192倍減、DNRが9.4倍増。
Related Reference	ACC Q9ALN6 NITE Spino_00070 PmId [18345667] In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa. (J Am Chem Soc. , 2008) comment Blast 28th, id49%, 1e-114 Saccharopolyspora spinosa_spnO Probable NDP-hexose-2,3-dehydratase dTDP-4-keto-6-deoxy-D-glucose 2,3-dehydratase_SpnO dTDP-D-glucose, RfbB(dTDP-D-glucose→dTDP-4-keto-6-deoxy-D-glucose), and either NADH or NADPHと一緒にSpnO and SpnNをインキュベートし、NAD(P)Hの消費量をフォローしてtandem reactionを測定。SpnO or SpnNのどちらかを欠いた場合、消費が見られなかった。 dTDP-3,4-diketo-2,6-dideoxy-D-glucoseは不安定で急速にdTDP and maltolに分解してしまうため、SpnOのみの触媒効率は定量できていない。 -- SpnNはdTDP-3,4-diketo-2,6-dideoxy-D-glucose 3-ketoreductaseであることが他で報告されている。

Sequence

>putative NDP-hexose 2,3-dehydratase [doxorubicin biosynthesis protein DnmT]
MTAQIARSVLARDGLGSGMDRFWAWYADRSAQVVHRTERIPLDDLKGWSRHPVTGTVSHH
TGRFFSIEGLDVHIPGAPVPRWSQPIVNQPEVGILGFLVKERHGVLHCLVQAKFEPGNPG
GLQLSPTVQATRSNYTRVHGGKAIPYLEHFRDTAERQVVADVLQSEQGSWFYRKRNRNMI
VQVIDEVPLHEDFHWLTLGQLHRLLGVENLVNMDARTVLACLPFASEGAHPLADAEPAGA
DGPGGGHPVVPARPDGGEGRVDTGFHRSLVRSCAAAEGSLHSTVDIVSWIADLRSRTDVV
TRPAALNALPHWYERDGAIAHESGRFLEVMAVDVTAASREVPGWSQPMIEPKDQGVAAFL
VRRIDGVLHVLAHARVEPGYVDVVEIAPTVQCTPGSLHALPAEARPRFLDAVLEAPPERV
RYATVLAEEGGRFYHAVNTYMIVEADHDIPDGGEYRWLTLHQLVGLLRHSHYVNVQARTL
VACLHSLSVGSPVTRSAVPSAPPRR

>putative NDP-hexose 2,3-dehydratase [doxorubicin biosynthesis protein DnmT]
ATGACCGCCCAGATCGCCCGGTCCGTCCTCGCCCGGGACGGCCTGGGCAGCGGCATGGAC
CGTTTCTGGGCGTGGTACGCCGACCGCTCGGCGCAGGTCGTGCACCGCACCGAGCGGATT
CCGCTGGACGACCTCAAGGGGTGGAGCCGCCACCCGGTCACTGGGACCGTCTCCCATCAC
ACCGGGAGGTTCTTCAGCATCGAGGGACTGGACGTGCACATCCCGGGCGCCCCCGTGCCA
CGCTGGTCCCAGCCGATCGTGAACCAGCCCGAGGTGGGCATCCTCGGCTTCCTGGTGAAG
GAGCGCCACGGGGTCCTGCACTGCCTCGTCCAGGCCAAGTTCGAGCCGGGCAACCCCGGC
GGGCTTCAGCTCTCCCCTACGGTCCAGGCCACCCGCAGCAACTACACGCGGGTGCACGGC
GGCAAGGCCATCCCGTACCTGGAGCACTTCCGCGACACCGCCGAACGGCAGGTGGTCGCC
GATGTGCTCCAGTCCGAACAGGGCTCCTGGTTCTACCGCAAGCGCAACCGCAACATGATC
GTCCAAGTGATCGACGAGGTCCCGCTGCACGAGGACTTCCACTGGCTGACGCTGGGTCAG
CTGCACCGGCTGCTGGGGGTCGAGAACCTCGTGAACATGGACGCCAGGACGGTGCTGGCC
TGCCTGCCGTTCGCGAGTGAGGGAGCGCACCCGCTGGCCGACGCGGAGCCGGCCGGAGCC
GACGGCCCCGGAGGTGGCCACCCGGTGGTCCCCGCTCGACCCGACGGCGGCGAGGGCCGG
GTAGACACCGGTTTCCACCGCTCCCTCGTGCGGTCGTGCGCGGCGGCCGAGGGCAGCCTG
CACAGCACCGTGGACATCGTCAGCTGGATCGCCGACCTGCGCAGCCGTACCGACGTGGTC
ACCCGCCCGGCTGCGCTGAACGCGCTGCCGCACTGGTACGAGCGGGACGGCGCGATCGCC
CACGAGAGCGGGCGCTTCCTGGAGGTGATGGCCGTCGACGTGACGGCCGCCTCGCGGGAG
GTGCCCGGCTGGAGCCAGCCGATGATCGAACCCAAGGACCAAGGGGTCGCGGCTTTCCTG
GTCCGACGGATCGACGGGGTTCTCCACGTCCTGGCGCACGCGCGCGTGGAGCCCGGCTAC
GTCGACGTGGTGGAGATCGCGCCCACCGTACAGTGCACCCCCGGCAGTCTGCACGCCCTG
CCCGCCGAGGCCAGGCCCCGCTTCCTCGACGCCGTGCTGGAGGCTCCACCCGAGCGCGTG
CGCTACGCGACCGTCCTCGCGGAGGAGGGAGGGCGCTTCTACCACGCGGTGAACACCTAC
ATGATCGTCGAAGCCGACCATGACATCCCGGACGGTGGCGAATACCGCTGGCTGACGCTC
CACCAACTGGTCGGGCTGCTGCGTCACTCCCACTACGTCAACGTCCAGGCCCGCACCCTG
GTCGCCTGTCTGCACAGCCTGTCCGTGGGCTCGCCCGTAACCCGCTCAGCCGTGCCGTCC
GCCCCGCCCCGGAGGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005212 NDP-hexose 2,3-dehydratase (Domain) PF03559 Hexose_dehydrat
SignalP	Bacteria, Gram-negative
TMHMM	No significant hit

Adria_00050 Adria_00070