Adria_00080 : CDS information

Location

Organism	Streptomyces peucetius
Strain	ATCC 29050 (=NBRC 100596)
Entry name	Doxorubicin
Contig	L47163
Start / Stop / Direction	655 / 1,710 / + [in whole cluster] 655 / 1,710 / + [in contig]
Location	655..1710 [in whole cluster] 655..1710 [in contig]
Type	CDS
Length	1,056 bp (351 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative glucose-1-phosphate thymidylyltransferase putative dTDP-glucose synthase
Product (GenBank)	glucose-1-phosphate thymidylyltransferase
Gene	dnmL
Gene (GenBank)	dnrL
EC number	2.7.7.24
Keyword	daunosamine
Note
Note (GenBank)	putative
Reference	ACC Q54819 PmId [8932700] The dnrM gene in Streptomyces peucetius contains a naturally occurring frameshift mutation that is suppressed by another locus outside of the daunorubicin-production gene cluster. (Microbiology. , 1996) comment dnrMの解析論文。一緒にdnrLも同定されている。高い配列類似性から、DnrLはglucose-1-phosphate thymidylyl transferaseであると提唱されている。 dnrLについては配列解析のみ。
Related Reference	ACC Q194R4 NITE Mith_00060 PmId [9150235] Cloning and insertional inactivation of Streptomyces argillaceus genes involved in the earliest steps of biosynthesis of the sugar moieties of the antitumor polyketide mithramycin. (J Bacteriol. , 1997) comment Blast 4th, id67%, 1e-127 Streptomyces argillaceus_mtmD DTDP-glucose synthase E. coli発現タンパクで以下の2酵素の活性みている。 mtmD: glucose-1-phosphate:dTTP thymidylyl transferase mtmE: dTDP-D-glucose 4,6-dehydratase mtmD mutant -- accumulates premithramycinone

Sequence

>putative glucose-1-phosphate thymidylyltransferase [glucose-1-phosphate thymidylyltransferase]
MKALVLSGGSGTRLRPFTHTSPKQLVPVANKPVLYYVLEDIAQASITEVGIVVGETSNEI
RKAVGSGDRFGLRVTYLPQEAPLGLAHAVLIARDYLGEEDFVMYLGDNFVVGGIAGNSST
FRAERPDAQILLTRVSDPSSFGVAEIGCDGRVVALEEKPRHPRSDLAVVGIYLFTPVVHE
AVRALTPSRRGELEITDALQWLLDGPYDVRYTTISGYWKDTGNVADMLEVNRAVLDGIEP
GMEGQADAASELVGRVRIEAGAQIRASRIVARRHRAGRVTDRTSALHVDRGDCSIETARS
SLHHAGRLPAHGTRRVQHSLLGRNVTVAPAPRVPAGSRLILGDDSRVEISS

>putative glucose-1-phosphate thymidylyltransferase [glucose-1-phosphate thymidylyltransferase]
ATGAAGGCTCTCGTCCTGTCCGGGGGGTCGGGCACGCGCCTGCGGCCCTTCACACACACG
TCTCCCAAGCAGTTGGTGCCGGTAGCCAACAAGCCGGTCCTCTACTACGTCCTCGAGGAC
ATCGCTCAGGCCAGCATCACCGAGGTCGGCATCGTCGTTGGGGAGACGTCCAACGAGATC
CGCAAGGCCGTCGGGAGCGGCGACAGATTCGGGCTCCGCGTCACCTACCTTCCGCAGGAG
GCGCCCCTGGGTCTGGCTCACGCTGTCCTCATCGCCCGCGACTATCTCGGCGAGGAGGAC
TTCGTCATGTATCTCGGGGACAACTTCGTGGTCGGCGGCATCGCAGGGAACTCGTCGACG
TTCCGCGCGGAGCGCCCCGATGCCCAGATCCTGCTCACCCGTGTCTCCGACCCGTCCAGC
TTCGGCGTGGCCGAGATCGGCTGTGACGGCCGGGTCGTCGCGCTGGAGGAGAAGCCGAGG
CACCCCCGCAGTGATCTCGCCGTGGTCGGGATCTACTTGTTCACGCCGGTCGTGCACGAG
GCGGTGCGCGCCCTCACACCGTCACGGCGGGGCGAGCTGGAAATCACGGACGCCCTCCAG
TGGCTCCTCGACGGACCCTACGACGTGCGGTACACCACCATCTCCGGTTATTGGAAGGAC
ACGGGAAACGTCGCCGACATGCTGGAGGTGAACCGTGCCGTGCTGGACGGGATCGAGCCG
GGCATGGAGGGCCAGGCCGACGCGGCGAGCGAACTGGTGGGGCGGGTGCGGATCGAGGCG
GGCGCGCAGATCCGGGCGTCGCGCATCGTGGCCCGCCGTCATCGGGCCGGACGTGTGACG
GATCGTACGTCGGCCCTACACGTCGATCGCGGAGACTGCTCGATCGAGACAGCGAGATCG
AGTCTCCATCATGCTGGGCGACTCCCGGCTCACGGCACGCGCCGGGTCCAGCACTCGCTC
CTGGGGCGGAACGTGACCGTCGCTCCGGCCCCCCGGGTACCCGCAGGTTCCCGTCTCATC
CTCGGTGACGACAGCAGGGTGGAGATCTCCTCATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005835 Nucleotidyl transferase (Domain) PF00483 NTP_transferase IPR005908 Glucose-1-phosphate thymidylyltransferase, long form (Family) TIGR01208 RmlA_long
SignalP	No significant hit
TMHMM	No significant hit

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