Adria_00090 : CDS information

Location

Organism	Streptomyces peucetius
Strain	ATCC 29050 (=NBRC 100596)
Entry name	Doxorubicin
Contig	L47163
Start / Stop / Direction	1,707 / 1,892 / + [in whole cluster] 1,707 / 1,892 / + [in contig]
Location	1707..1892 [in whole cluster] 1707..1892 [in contig]
Type	CDS
Length	186 bp (61 aa)

Annotation

Category	5.2 function unknown
Product	putative dTDP-glucose 4,6-dehydratase(fragment)
Product (GenBank)
Gene	dnmM
Gene (GenBank)	dnrM
EC number
Keyword
Note	This ORF may be a truncated form of dTDP-glucose 4,6-dehydratase because of a frameshift mutation.
Note (GenBank)	truncated
Reference	ACC Q54820 PmId [8932700] The dnrM gene in Streptomyces peucetius contains a naturally occurring frameshift mutation that is suppressed by another locus outside of the daunorubicin-production gene cluster. (Microbiology. , 1996) comment DnrMはdTDP-D-glucose dehydratasesに高い配列類似性を示すが、翻訳の中途終止を引き起こすDNA配列フレームシフトがある。 dnrMの不活化、相補実験によっても、Daunorubicin (DNR)生合成に関与しないことが確認されている。 Doxorubicin (DXR) 産生株Streptomyces peucetius ATCC 27952において、rmbBがこの機能を担うことが確認されている[PMID: 19777229]。

Sequence

>putative dTDP-glucose 4,6-dehydratase(fragment)
MTMKILVTGGAGFIGSHYVRALLGPRGDGSVGVTAGQPDLCRQSGQPRSRPRRRAVRLRP
R

>putative dTDP-glucose 4,6-dehydratase(fragment)
ATGACCATGAAGATCCTGGTGACAGGCGGTGCCGGCTTCATCGGCTCGCACTACGTCCGC
GCCCTGCTCGGCCCGCGCGGCGACGGCTCGGTCGGGGTCACTGCTGGACAGCCTGACCTA
TGCAGGCAATCCGGCCAACCTCGATCCCGTCCGCGGCGACGAGCGGTTCGCCTTCGTCCA
CGGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001509 NAD-dependent epimerase/dehydratase (Domain) PF01370 Epimerase IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 no description
SignalP	Eukaryota Bacteria, Gram-negative
TMHMM	No significant hit

Adria_00080 Adria_00100