Adria_00160 Adria_00160   Adria_00190 Adria_00190

Adria_00180 : CDS information

close this sectionLocation

Organism
StrainATCC 29050 (=NBRC 100596)
Entry nameDoxorubicin
Contig
Start / Stop / Direction12,060 / 13,088 / + [in whole cluster]
25 / 1,053 / + [in contig]
Location12060..13088 [in whole cluster]
25..1053 [in contig]
TypeCDS
Length1,029 bp (342 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
ProductdTDP-L-epivancosamine nitrososynthase
FAD-dependent oxidoreductase
Product (GenBank)putative flavoprotein
Gene
Gene (GenBank)dnmZ
EC number
Keyword
  • baumycin
Note
Note (GenBank)
  • DnmZ
Reference
ACC
PmId
[9209071] Cloning and characterization of the Streptomyces peucetius dnmZUV genes encoding three enzymes required for biosynthesis of the daunorubicin precursor thymidine diphospho-L-daunosamine. (J Bacteriol. , 1997)
[10631513] A two-plasmid system for the glycosylation of polyketide antibiotics: bioconversion of epsilon-rhodomycinone to rhodomycin D. (Chem Biol. , 1999)
[23885759] Nitrososynthase-triggered oxidative carbon-carbon bond cleavage in baumycin biosynthesis. (J Am Chem Soc. , 2013)
[26457508] Structure of DnmZ, a nitrososynthase in the Streptomyces peucetius anthracycline biosynthetic pathway. (Acta Crystallogr F Struct Biol Commun. , 2015)
comment
[PMID: 9209071](1997)
dnmZ: Unknown

dnmZ::aphII strainは最終aglyconeのRHOを蓄積。
このmutantへのdnmZ or dnmUVの導入はDaunorubicin (DNR)産生を回復せず。dnmZUVの導入で回復。

acyl-CoA dehydrogenaseとのidは低く、実験によっても否定されている。

---
同じ著者らの続報。
[PMID: 10631513](1999)
dnmZ: Unknown; putative flavoprotein

S.peucetius mutant WMH1629(dnmZ::aphII, dnmU, dnmZ)はRHO蓄積。
pWHM1906(dnrI+dnmJVUZTQS)導入で相補されDNR/DXR産生回復。
pWHM1916(dnrI+dnmJVUZHTWQSのdnmZをin-frame deletionしたもの)導入でもDNR/DXR産生はwildと変わらず。
polar effectは、dnmZについては述べていない。

異種性ホストS.lividansにおけるL-daunosamineの生合成と付着のための最少の情報はdnmLMJVUTS genesによってコードされ、dnmZ and dnrHは必要ではない。dnmW and dnmQは絶対的に必須というわけではない。（ただ、S.lividansが代替酵素を持っているための結果なのかは、精製された酵素での実験をしてみないとわからない。）
Related Reference
ACC
A0A0R4I990
PmId
[26457508] Structure of DnmZ, a nitrososynthase in the Streptomyces peucetius anthracycline biosynthetic pathway. (Acta Crystallogr F Struct Biol Commun. , 2015)

close this sectionSequence

selected fasta
>dTDP-L-epivancosamine nitrososynthase [putative flavoprotein]
MTKPSVHEHPGVLADNGLCRPKTPAGRRLLDLLERYLPALEAESRDNDREATLPVHLFDR
MRKEGVLGATVPEDLGGLGVHSLHDVALALARIAGRDAGVALALHMQFSRGLTLDFEWRH
GAPSTRLEEAGAPVRLAAPVVARTTPGLTVLDNWDGMGMRSSGSVDIVFDGCP-DRDRVL
PRRRAGRRDDAALAGQTSARTHAGYHSATPRRSRIALTELRRRGGAPAGVRTTVAEIDAR
LFALHTAVASALTTADRLADDLSGDLAARGRAMMTPFQYAKLLVNRHSVGVVDDCLMLVG
GAGYSNSHPLARLYRDVRAGGFMHPYNFTDGVDYLSEVALGR
selected fasta
>dTDP-L-epivancosamine nitrososynthase [putative flavoprotein]
ATGACAAAGCCATCTGTGCACGAACACCCCGGTGTCCTGGCCGACAACGGCCTCTGCAGA
CCCAAGACGCCGGCCGGGCGACGGCTGCTCGACCTTCTGGAGCGGTACCTGCCGGCCCTG
GAGGCGGAGAGCCGGGACAACGACCGCGAGGCCACTCTGCCCGTCCACCTCTTCGACCGG
ATGCGCAAGGAGGGCGTGCTGGGGGCCACCGTCCCGGAGGACCTCGGAGGGCTCGGCGTC
CACTCGTTGCACGACGTCGCGCTGGCCCTGGCCAGGATCGCCGGCCGGGACGCGGGCGTG
GCGCTGGCTCTGCACATGCAGTTCAGCCGGGGTCTGACCCTGGACTTCGAGTGGCGGCAC
GGGGCACCGTCGACCCGGCTGGAGGAGGCGGGCGCCCCCGTCCGGCTGGCCGCACCGGTG
GTCGCGCGCACCACACCGGGCCTGACCGTACTGGACAACTGGGACGGAATGGGCATGCGG
TCGTCGGGCAGTGTGGACATCGTCTTCGACGGCTGTCCGNTGGACCGGGACCGGGTGCTG
CCGCGACGGCGAGCCGGGCGTCGCGACGACGCGGCGCTCGCCGGGCAGACGTCAGCTCGT
ACGCATGCTGGCTATCACTCGGCTACGCCGAGGCGCTCGAGGATCGCCCTCACGGAACTG
CGCAGGCGCGGCGGGGCGCCGGCCGGGGTGCGGACCACGGTGGCCGAGATAGACGCCCGG
CTGTTCGCGCTGCACACGGCGGTGGCGAGCGCGTTGACCACCGCCGACCGGCTCGCCGAC
GACCTCAGCGGCGACCTCGCCGCCCGCGGCCGCGCCATGATGACGCCCTTCCAGTACGCC
AAGCTGCTCGTCAACCGGCACTCGGTGGGCGTGGTGGACGACTGCCTGATGCTCGTGGGA
GGGGCCGGATACAGCAACTCCCACCCGCTGGCGCGCCTGTACCGCGATGTCCGGGCGGGC
GGGTTCATGCATCCATACAACTTCACGGACGGCGTCGACTACCTGAGCGAAGTGGCGTTG
GGCCGATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR006090 Acyl-CoA oxidase/dehydrogenase, type 1 (Domain)
[225-321] 1.2e-09 PF00441
PF00441   Acyl-CoA_dh_1
IPR006091 Acyl-CoA oxidase/dehydrogenase, central domain (Domain)
[131-180] 4.3e-09 G3DSA:2.40.110.10
G3DSA:2.40.110.10   Acyl_CoA_DH/ox_M
IPR006092 Acyl-CoA dehydrogenase, N-terminal (Domain)
[40-122] 3.1e-09 PF02771
PF02771   Acyl-CoA_dh_N
IPR009075 Acyl-CoA dehydrogenase/oxidase C-terminal (Domain)
[221-325] 1.80000149287196e-10 SSF47203
SSF47203   AcylCoADH_C_like
[201-328] 3.2e-14 G3DSA:1.20.140.10
G3DSA:1.20.140.10   AcylCoA_DH_1/2_C
IPR009100 Acyl-CoA dehydrogenase/oxidase (Domain)
[23-184] 9.5000102816722e-18 SSF56645
SSF56645   AcylCoA_dehyd_NM
IPR013786 Acyl-CoA dehydrogenase/oxidase, N-terminal (Domain)
[23-110] 4.6e-13 G3DSA:1.10.540.10
G3DSA:1.10.540.10   AcylCoA_DH/ox_N
SignalP No significant hit
TMHMM No significant hit
Adria_00160 Adria_00160   Adria_00190 Adria_00190
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