Adria_00260 Adria_00260   Adria_00280 Adria_00280

Adria_00270 : CDS information

close this sectionLocation

Organism
StrainATCC 29050 (=NBRC 100596)
Entry nameDoxorubicin
Contig
Start / Stop / Direction27,248 / 28,507 / + [in whole cluster]
3,258 / 4,517 / + [in contig]
Location27248..28507 [in whole cluster]
3258..4517 [in contig]
TypeCDS
Length1,260 bp (419 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase beta-ketoacyl synthase subunit
Product (GenBank)daunorubicin-doxorubicin polyketide synthase
Gene
Gene (GenBank)dpsA
EC number
Keyword
  • type II PKS
Note
Note (GenBank)
  • protein A
Reference
ACC
PmId
[7828855] Characterization of the Streptomyces peucetius ATCC 29050 genes encoding doxorubicin polyketide synthase. (Gene. , 1994)
comment
daunorubicin (DNR)-doxorubicin (DXR) PKSを含む領域9ORFsの同定論文。

dpsA: 3-oxoacyl:ACP synthase
配列解析のみ。
Related Reference
ACC
Q55223
NITE
Dauno_00160
PmId
[9098068] Minimal Streptomyces sp. strain C5 daunorubicin polyketide biosynthesis genes required for aklanonic acid biosynthesis. (J Bacteriol. , 1997)
comment
Blast 2nd, id95%, 0.0
Streptomyces sp.(Strain C5)_dauA
Polyketide synthase

daunorubicin (DNR), doxorubicin (DXR)の前駆体であるaklanonic acidを異種性ホストで産生するために必要な最小遺伝子は、dpsG (ACP), dauI (regulatory activator), dpsA (KS alpha), dpsB (KS beta), dpsF (aromatase), dpsE (polyketide reductase), and dauG (putative deoxyaklanonic acid oxygenase)であるということを実証している。

dpsA(KS alpha)がこのentryに相当。

close this sectionPKS/NRPS Module

KS3..375

close this sectionSequence

selected fasta
>polyketide synthase beta-ketoacyl synthase subunit [daunorubicin-doxorubicin polyketide synthase]
MNRRIVITGIGVVAPGAVGTKPFWELLLSGTTATRAISTFDATPFRSRIAAECDFDPVAA
GLSAEQARRLDRAGQFALVAGQEALADSGLRIDEDSAHRVGVCVGTAVGCTQKLESEYVA
LSAGGAHWVVDPGRGSPELYDYFVPSSLAAEVAWLAGAEGPVNIVSAGCTSGIDSIGYAC
ELIREGTVDAMVAGGVDAPIAPITVACFDAIRATSDHNDTPETASRPFSRSRNGFVLGEG
GAIVVLEEAEAAVRRGARIYAEIGGYASRGNAYHMTGLRADGAEMAAAITAALDEARRDP
SDVDYVNAHGTATKQNDRHETSAFKRSLGEHAYRVPISSIKSMIGHSLGAVGSLEVAATA
LAVEYGVIPPTANLHDPDPELDLDYVPLTAREKRVRHALTVGSGFGGFQSAMLLSRLER
selected fasta
>polyketide synthase beta-ketoacyl synthase subunit [daunorubicin-doxorubicin polyketide synthase]
GTGAACCGGCGGATCGTCATCACCGGGATCGGCGTGGTGGCTCCCGGCGCCGTCGGGACC
AAGCCGTTCTGGGAGCTGCTGCTGTCCGGAACCACCGCCACCCGCGCCATCAGCACTTTC
GACGCCACGCCCTTCCGTTCCCGTATCGCCGCCGAGTGCGACTTCGATCCGGTGGCGGCG
GGGCTGTCCGCGGAGCAGGCCCGGCGCCTCGACCGGGCCGGGCAGTTCGCGCTGGTCGCC
GGTCAGGAGGCGCTCGCCGACAGCGGGCTGCGAATCGATGAGGACTCCGCGCACCGGGTC
GGGGTGTGCGTGGGCACCGCCGTCGGCTGCACGCAGAAACTGGAGTCCGAGTACGTCGCT
CTCAGCGCGGGCGGAGCGCACTGGGTCGTGGACCCCGGCCGGGGGTCGCCCGAGCTGTAC
GACTACTTCGTGCCGAGTTCCCTCGCCGCCGAAGTGGCCTGGCTCGCCGGGGCGGAGGGG
CCCGTGAACATCGTCTCGGCCGGGTGCACCTCGGGGATCGACTCCATCGGGTACGCCTGC
GAGCTCATCCGCGAGGGGACGGTGGACGCCATGGTCGCGGGAGGCGTGGACGCACCCATC
GCGCCGATCACGGTGGCCTGCTTCGACGCCATCAGGGCGACCTCCGACCACAACGACACC
CCCGAGACCGCCTCGCGTCCCTTCAGCCGCAGCCGCAACGGCTTTGTGCTGGGCGAGGGC
GGTGCGATCGTGGTCCTGGAAGAGGCGGAGGCGGCCGTGCGCAGGGGCGCGCGGATCTAC
GCCGAGATCGGCGGGTACGCCTCACGCGGCAACGCGTACCACATGACCGGGCTGCGCGCA
GACGGCGCCGAGATGGCGGCGGCGATCACCGCGGCCCTCGACGAGGCCCGCCGCGACCCC
TCGGACGTGGACTACGTCAACGCCCACGGAACGGCGACGAAGCAGAACGACCGGCACGAG
ACCTCCGCGTTCAAGCGCTCCCTGGGGGAGCACGCGTACCGGGTGCCCATCAGCTCCATC
AAGTCGATGATCGGTCACTCCCTGGGCGCTGTCGGCTCGCTCGAAGTGGCGGCGACCGCA
CTGGCCGTGGAGTACGGGGTGATCCCGCCGACCGCGAATCTGCACGACCCGGATCCGGAA
CTCGACCTGGACTACGTGCCGCTGACGGCACGAGAAAAGCGGGTGCGGCACGCGCTGACG
GTGGGCAGCGGCTTCGGCGGCTTCCAGAGCGCCATGCTGCTGAGCCGTCTCGAGAGGTGA
KS3..375
KS7..1125

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[3-251] 9.40000000000003e-60 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[260-375] 3.60000000000002e-38 PF02801
PF02801   Ketoacyl-synt_C
IPR016038 Thiolase-like, subgroup (Domain)
[2-263] 1.2e-64 G3DSA:3.40.47.10 [270-417] 6.29999999999998e-52 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[2-257] 5.50001754266469e-67 SSF53901 [219-418] 3.89999861795218e-64 SSF53901
SSF53901   Thiolase-like
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
[160-176] PS00606
PS00606   B_KETOACYL_SYNTHASE
SignalP
[1-19] 0.845 Signal
Eukaryota   
[1-20] 0.582 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Adria_00260 Adria_00260   Adria_00280 Adria_00280
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