Adria_00310 : CDS information

Location

Organism	Streptomyces peucetius
Strain	ATCC 29050 (=NBRC 100596)
Entry name	Doxorubicin
Contig	L35560
Start / Stop / Direction	32,710 / 31,850 / - [in whole cluster] 8,720 / 7,860 / - [in contig]
Location	complement(31850..32710) [in whole cluster] complement(7860..8720) [in contig]
Type	CDS
Length	861 bp (286 aa)

Annotation

Category	3.2 modification methylation
Product	aklanonic acid O-methyltransferase
Product (GenBank)
Gene	dnrC
Gene (GenBank)
EC number	2.1.1.-
Keyword
Note
Note (GenBank)	ORF5
Reference	ACC Q54818 PmId [7828855] Characterization of the Streptomyces peucetius ATCC 29050 genes encoding doxorubicin polyketide synthase. (Gene. , 1994) [7601857] Functional characterization and transcriptional analysis of a gene cluster governing early and late steps in daunorubicin biosynthesis in Streptomyces peucetius. (J Bacteriol. , 1995) comment [PMID: 7828855](1994) daunorubicin (DNR)-doxorubicin (DXR) PKSを含む領域9ORFsの同定論文。 ORF5については配列解析のみ。 ORF5にはっきりと提唱された機能なし。 --- [PMID: 7601857](1995) ORF5＝dnrC Streptomyces lividans TK24にpWHM918 (carrying dnrC)導入。この株はaklanonic acid → aklanonic acid methylester(AAME)の変換ができる。この変換でできた産物をdpsB(-)mutantに加えると、RHOへ変換可能。よって正しい中間体。これらの演繹は、相同なStreptomyces sp. strain C5 dauC geneの機能と完全に一致する。
Related Reference	ACC Q55214 NITE Dauno_00080 PmId [7836284] Analysis of clustered genes encoding both early and late steps in daunomycin biosynthesis by Streptomyces sp. strain C5. (J Bacteriol. , 1995) comment Blast 2nd, id94%, 1e-110 Streptomyces sp.(strain C5)_dauC Aklanonic acid methyltransferase dauCは、Streptomyces sp. strain C5のdauC-blocked mutantsを相補し、vitroでmutant strainにaklanonic acid methyltransferase活性を回復し、vitroでStreptomyces lividansにaklanonic acid methyltransferase活性を与える。

Sequence

>aklanonic acid O-methyltransferase
MQDSSYKEQVTQAFDQSSSTYDRLGVEFFTPMGRPLVEISEPVTGERVLDIGCGRGACLF
PAAEKVGPQGRVHGIDIAPGMIEEARKEAAERGLRNIALDVMDAETPELPARSFDLVMGS
YSVIFLPDAVGALARYAGILDHGGRIAFTSPVFRAGTFPFLPPEFTPLIPQALLEHLPEQ
WRPEALVRRFNSWLERAEDLLRTLERCGYTSVAVTDEPVRMTALSSEAWVDWSHTQGMRL
LWQNLPQAQRTELRARLVEGLDKLSDATGALAIDVPVRFVTARVAH

>aklanonic acid O-methyltransferase
GTGCAGGATTCGTCCTACAAAGAGCAGGTAACTCAGGCCTTCGACCAGTCGTCGTCGACC
TACGACCGACTGGGAGTGGAATTCTTCACGCCCATGGGTCGGCCCCTGGTGGAAATCTCG
GAGCCGGTCACCGGTGAACGAGTGCTCGACATCGGCTGCGGGCGCGGCGCATGCCTTTTT
CCGGCTGCGGAAAAGGTCGGTCCTCAGGGGCGCGTCCACGGAATCGACATTGCTCCCGGA
ATGATCGAGGAAGCTCGCAAGGAAGCCGCCGAACGCGGTCTGCGGAATATCGCCCTCGAT
GTGATGGACGCGGAGACGCCCGAACTCCCGGCGCGCTCCTTCGACCTGGTCATGGGGAGC
TACAGCGTCATCTTCCTGCCGGACGCGGTGGGGGCGCTGGCGCGCTACGCCGGAATCCTG
GACCACGGCGGCAGGATCGCGTTCACCAGCCCGGTCTTCCGCGCCGGCACGTTCCCGTTC
CTGCCACCGGAGTTCACCCCGCTCATCCCGCAGGCGCTGCTGGAGCACCTGCCCGAGCAG
TGGCGGCCCGAGGCGCTGGTCCGGCGGTTCAACAGCTGGCTGGAGCGGGCCGAGGATCTC
CTCCGCACCCTGGAACGCTGCGGCTACACCTCGGTCGCCGTCACCGACGAGCCGGTGCGG
ATGACGGCCCTGTCCAGCGAGGCGTGGGTGGACTGGTCCCACACCCAGGGCATGCGTCTG
CTGTGGCAGAACCTCCCGCAGGCCCAGCGAACGGAACTGCGCGCACGGCTCGTCGAGGGA
CTCGACAAGCTGAGCGACGCCACCGGGGCCCTCGCCATCGACGTGCCCGTCCGCTTCGTC
ACCGCCCGGGTCGCCCACTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	No significant hit
SignalP	No significant hit
TMHMM	No significant hit

Adria_00300 Adria_00320