Adria_00340 : CDS information

Location

Organism	Streptomyces peucetius
Strain	ATCC 29050 (=NBRC 100596)
Entry name	Doxorubicin
Contig	L40425
Start / Stop / Direction	34,478 / 35,374 / + [in whole cluster] 1,751 / 2,647 / + [in contig]
Location	34478..35374 [in whole cluster] 1751..2647 [in contig]
Type	CDS
Length	897 bp (298 aa)

Annotation

Category	3.4 other modification
Product	putative rhodomycin D esterase
Product (GenBank)	10-carbomethoxy-13-deoxycarminomycin esterase
Gene
Gene (GenBank)	dnrP
EC number
Keyword
Note
Note (GenBank)	putative
Reference	ACC Q54809 PmId [7601857] Functional characterization and transcriptional analysis of a gene cluster governing early and late steps in daunorubicin biosynthesis in Streptomyces peucetius. (J Bacteriol. , 1995) comment DnrPはesterasesに相同性があり、 10-carbomethoxy-13-deoxycarminomycin → 10-carboxy-13-deoxycarminomycin への変換を触媒するかもしれない。 dnrP単独の不活化mutant作成はできていない。 dnrKへのaphII gene insertによりdnrKPQSが不活化されていると示唆されるWMH1536 strainでの相補による産物解析から、消去法でDnrPは最初のglycosylateされた中間体の合成の後に働くと結論付けている。これが上記の反応。
Related Reference	ACC Q55217 NITE Dauno_00110 PmId [9098063] In vivo and in vitro bioconversion of epsilon-rhodomycinone glycoside to doxorubicin: functions of DauP, DauK, and DoxA. (J Bacteriol. , 1997) comment Blast 2nd, id95%, 1e-160 Streptomyces sp.(strain C5)_dauP Esterase [DoBISCUIT]rhodomycin D esterase dauP: rhodomycin D esterase rhodomycin D → 10-carboxy-13-deoxycarminomycin 続いて10-carboxy-13-deoxycarminomycin → 13-deoxycarminomycinが自発的に起こり、carbomethoxy基の除去をする。 dauP発現株で変換を確認。 4-O-methylrhodomycin D → 10-carboxy-13-deoxydaunorubicinの変換も見ている。

Sequence

>putative rhodomycin D esterase [10-carbomethoxy-13-deoxycarminomycin esterase]
MPTRMITNDEVTLWSEGLGDPADAPLLLIAGGNLSAKSWPDEFVERLVAAGHFVIRYDHR
DTGRSSRCDFALHPYGFDELAADALAVLDGWQVRAAHVVGMSLGNTIGQLLALDAPERLL
TLTVMLGGALDVDFDADLEAALKGEPSVSGLPVPSQRFLDMMTMLQQPAETDEELLERRV
EKWRLLNGEGVPFDADEFRRRELLAAGHAGTFDEPIVHHMIPLPPVSRGAELSRVTTPVL
AIQAMCDPAAPPPHARHLANRIPGARVVEIENMGHALPLAVHEPLAAAICAHTRAATV

>putative rhodomycin D esterase [10-carbomethoxy-13-deoxycarminomycin esterase]
ATGCCGACACGCATGATCACCAACGATGAGGTGACCCTGTGGAGCGAAGGGCTCGGCGAT
CCGGCCGACGCCCCGTTGCTCCTGATCGCCGGCGGCAACCTCTCGGCCAAATCGTGGCCG
GACGAGTTCGTCGAACGCCTGGTCGCGGCCGGGCACTTCGTGATCCGCTACGACCACCGG
GACACCGGGCGCTCCTCCCGGTGCGACTTCGCGCTCCACCCCTACGGCTTCGACGAGCTG
GCCGCCGACGCGCTGGCCGTCCTGGACGGCTGGCAGGTCCGCGCCGCCCATGTGGTGGGC
ATGTCGCTGGGCAACACCATCGGCCAGCTGCTCGCCCTGGACGCTCCGGAGCGTCTCCTC
ACACTCACCGTGATGCTGGGGGGCGCGCTCGACGTCGACTTCGACGCCGACCTGGAGGCG
GCGCTCAAGGGTGAGCCTTCCGTCAGCGGACTGCCGGTGCCCAGCCAGCGGTTCCTCGAC
ATGATGACGATGCTTCAGCAACCGGCCGAGACGGACGAGGAACTGCTGGAGCGCCGAGTG
GAGAAGTGGCGCCTACTCAACGGGGAGGGAGTGCCCTTCGACGCCGACGAGTTCCGGCGT
CGTGAACTCCTGGCCGCCGGGCACGCGGGAACGTTCGACGAGCCGATCGTGCACCACATG
ATCCCGCTGCCGCCGGTGTCGCGGGGGGCGGAGTTGTCCCGCGTCACCACACCGGTACTG
GCGATCCAGGCGATGTGCGACCCCGCGGCCCCGCCGCCGCACGCCCGGCACCTCGCCAAC
CGGATTCCCGGCGCCCGGGTCGTGGAGATCGAGAACATGGGGCACGCCCTGCCCCTCGCG
GTCCACGAGCCGCTGGCCGCCGCCATCTGCGCCCACACCCGGGCCGCGACAGTGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	No significant hit
SignalP	No significant hit
TMHMM	No significant hit

Adria_00330 Adria_00350