Dauno_00100 : CDS information

Location

Organism	Streptomyces sp.
Strain	C5
Entry name	Daunorubicin
Contig	L35154
Start / Stop / Direction	19,105 / 20,175 / + [in whole cluster] 1,835 / 2,905 / + [in contig]
Location	19105..20175 [in whole cluster] 1835..2905 [in contig]
Type	CDS
Length	1,071 bp (356 aa)

Annotation

Category	3.2 modification methylation
Product	C-4 O-methyltransferase
Product (GenBank)	carminomycin 4-O-methyltransferase
Gene
Gene (GenBank)	dauK
EC number	2.1.1.-
Keyword
Note
Note (GenBank)
Reference	ACC Q55216 PmId [7836284] Analysis of clustered genes encoding both early and late steps in daunomycin biosynthesis by Streptomyces sp. strain C5. (J Bacteriol. , 1995) [9098063] In vivo and in vitro bioconversion of epsilon-rhodomycinone glycoside to doxorubicin: functions of DauP, DauK, and DoxA. (J Bacteriol. , 1997) comment [PMID: 7836284](1995) Daunorubicin (DNR)のPKS genes領域に近接した4ORFsの同定。 Streptomyces sp. strain C5 dauKを含んだplasmidsはS. lividans TK24にCarminomycin 4-O-methyltransferase活性を与えた。 ↓同じ人たちの続報 --- [PMID: 9098063](1997) S. lividans TK24で発現したStreptomyces sp. strain C5 dauKの生物変換測定(in vivo/in vitro)。 rhodomycinD → 4-O-methyl-rhodomycinD 13-deoxy-carminomycin → 13-deoxy-daunorubicin 13-dihydro-carminomycin → 13-dihydro-daunorubicin carminomycin → daunorubicin への変換はin vivo/in vitroどちらでも100%(TABLE 3)。本文中には 10-carboxy-13-doexycarminomycin → 10-carboxy-13-deoxydaunorubicin も変換すると書いてある。このように基質幅が広いので、carminomycin 4-O-methyltransferaseからanthracycline 4-O-methyltransferaseへ改名することを勧めている。
Related Reference	ACC Q06528 NITE Adria_00330 PmId [8509343] Cloning and sequencing of a gene encoding carminomycin 4-O-methyltransferase from Streptomyces peucetius and its expression in Escherichia coli. (J Bacteriol. , 1993) [7601857] Functional characterization and transcriptional analysis of a gene cluster governing early and late steps in daunorubicin biosynthesis in Streptomyces peucetius. (J Bacteriol. , 1995) [15273252] Crystal structure of a ternary complex of DnrK, a methyltransferase in daunorubicin biosynthesis, with bound products. (J Biol Chem. , 2004) comment Blast 2nd, id97%, 0.0 Streptomyces peucetius_dnrK Carminomycin 4-O-methyltransferase -- [PMID: 8509343](1993) E.coli、S.lividansで発現、精製したDnrKのcarminomycin 4-O-methyltransferase活性確認あり。 cofactor SAMのbinding site保存あり。 -- [PMID: 7601857](1995) PMID: 8509343ではD184A変異でDnrK活性を完全になくしたが、 dnrK geneのin-frame insertion mutationによるmutantはRHOを蓄積し、carminomycinを蓄積しない。 dnrKで補完されdaunorubicin (DNR)産生を回復するのでdnrKのみが不活化されている。 carminomycin不在は急速な分解のせいではないことも確認している(data not shown)。この結果はDnrKの2つめの知られていない機能を反映するのかもしれない。 -- [PMID: 15273252](2004) 結晶構造解析。

Sequence

>C-4 O-methyltransferase [carminomycin 4-O-methyltransferase]
MTAEPTVAARPQQIDALRTLIRLGSLHTPMVVRTAATLRLVDHILAGARTVKALAARTDT
RPEALLRLIRHLVAIGLLEEDAPGEFAPTEVGKLLADDHPAAQRAWHDLTQAVARADISF
TRLPEAIRSGRPTYESVYGKPFYEDLAGRPDLRASFDSLLACDQDVAFDAPAAAHDWTNV
RHVLDVGGGKGGFAAAIARRAPHVSATVLEMAGTVDTARSYLRDAGLSDRVDVVEGDFFE
PLPRRADAIILSFVLLNWPDHDAVRILTRCAEALEPGGRILIHERDDLHENSFNEQFTEL
DLRMLVFLGGALRTREKWDGLAASAGLVVEEVRQLPSPTIPYDLSLLVLAPASTGA

>C-4 O-methyltransferase [carminomycin 4-O-methyltransferase]
ATGACAGCCGAACCGACGGTCGCGGCCCGGCCGCAGCAGATCGACGCCCTCAGGACCCTG
ATCCGCCTCGGAAGCCTGCACACGCCCATGGTCGTCCGGACGGCTGCCACCCTGCGGCTC
GTCGACCACATCCTGGCGGGGGCCCGCACCGTGAAGGCCCTGGCGGCCAGAACCGACACG
CGGCCGGAAGCACTCCTGCGACTGATCCGCCACCTGGTGGCGATCGGCCTGCTCGAGGAG
GACGCACCGGGCGAGTTCGCCCCGACCGAGGTCGGCAAGCTGCTCGCCGACGACCACCCG
GCCGCTCAGCGTGCCTGGCACGACCTGACGCAGGCCGTGGCGCGTGCCGACATCTCCTTC
ACCCGCCTCCCCGAAGCCATCCGTAGCGGCCGCCCCACGTACGAGTCCGTCTACGGCAAG
CCGTTCTACGAAGATCTGGCCGGCCGCCCTGACCTGCGCGCGTCCTTCGACTCGCTGCTC
GCCTGCGACCAGGACGTCGCCTTCGACGCTCCGGCCGCCGCTCACGACTGGACGAACGTC
CGGCACGTGCTCGACGTGGGGGGTGGCAAGGGGGGGTTCGCCGCGGCCATCGCGCGCCGG
GCCCCACATGTGTCGGCCACCGTGCTGGAGATGGCGGGCACCGTGGACACCGCCCGCTCC
TACCTGAGGGACGCGGGCCTCTCCGACCGTGTCGACGTCGTCGAAGGGGACTTCTTCGAG
CCGCTGCCCCGCAGGGCGGACGCGATCATCCTCTCCTTCGTCCTCCTCAACTGGCCGGAT
CACGATGCTGTCCGAATCCTTACCCGCTGTGCCGAGGCCCTGGAGCCCGGCGGGCGGATC
CTGATCCACGAGCGCGACGACCTCCACGAGAACTCGTTCAACGAACAGTTCACCGAGCTC
GATCTGCGGATGCTGGTCTTCCTCGGCGGTGCCCTGCGCACCCGCGAGAAGTGGGACGGC
CTGGCCGCGTCGGCGGGCCTCGTGGTCGAGGAGGTACGGCAACTGCCTTCGCCGACCATC
CCGTACGACCTCTCGCTCCTCGTCCTCGCCCCCGCGTCCACCGGCGCTTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001077 O-methyltransferase, family 2 (Domain) PF00891 Methyltransf_2 IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain) G3DSA:1.10.10.10 Wing_hlx_DNA_bd IPR016461 O-methyltransferase, caffeic acid-type (Family) PIRSF005739 O-mtase
SignalP	Bacteria, Gram-positive
TMHMM	No significant hit

Dauno_00090 Dauno_00110