Dauno_00110 : CDS information

Location

Organism	Streptomyces sp.
Strain	C5
Entry name	Daunorubicin
Contig	L35154
Start / Stop / Direction	20,206 / 21,102 / + [in whole cluster] 2,936 / 3,832 / + [in contig]
Location	20206..21102 [in whole cluster] 2936..3832 [in contig]
Type	CDS
Length	897 bp (298 aa)

Annotation

Category	3.4 other modification
Product	rhodomycin D esterase
Product (GenBank)	esterase
Gene
Gene (GenBank)	dauP
EC number
Keyword
Note
Note (GenBank)	putative
Reference	ACC Q55217 PmId [7836284] Analysis of clustered genes encoding both early and late steps in daunomycin biosynthesis by Streptomyces sp. strain C5. (J Bacteriol. , 1995) [9098063] In vivo and in vitro bioconversion of epsilon-rhodomycinone glycoside to doxorubicin: functions of DauP, DauK, and DoxA. (J Bacteriol. , 1997) comment [PMID: 7836284](1995) Daunorubicin (DNR)のPKS genes領域に近接した4ORFsの同定。 DauPの機能予測は配列解析のみで、anthracycline 15-methyl esterase ↓同じ人たちの続報 --- [PMID: 9098063](1997) dauP: rhodomycin D esterase rhodomycin D → 10-carboxy-13-deoxycarminomycin 続いて10-carboxy-13-deoxycarminomycin → 13-deoxycarminomycinが自発的に起こり、carbomethoxy基の除去をする。 dauP発現株で変換を確認。 4-O-methylrhodomycin D → 10-carboxy-13-deoxydaunorubicinの変換も見ている。

Sequence

>rhodomycin D esterase [esterase]
MPTRMITKDEVTLWSEGIGDPADAPLLLIAGGNLSARSWPDEFVERLAAAGHFVIRYDHR
DTGRSSRYDFALHPYGFDELATDALAVLDAWQVRAAHVVGMSLGNTIGQLLALDAPERLL
TLTVMLGGALDVDFDADLEAALKGEPSVSGLPVPSRRFLDMMMLLQQPAGTDEELLERRV
EKWRLLNGEGVPFDSDEFRRRELLAAGHAGTFDEPIVHHMIPQPPVSRGAELARITTPVL
AIQAMCDPAAPPPHARHLADRIPGARVVEIENMGHALPLAVHEPLAAAICAHTRAATV

>rhodomycin D esterase [esterase]
ATGCCGACACGCATGATCACCAAGGACGAGGTGACCTTGTGGAGCGAAGGAATCGGCGAT
CCGGCCGACGCTCCGTTGCTCCTGATCGCCGGCGGCAACCTTTCGGCCAGGTCATGGCCG
GACGAGTTCGTTGAACGCCTCGCCGCGGCCGGGCACTTCGTGATCCGCTACGACCACCGG
GACACAGGGCGCTCCTCCCGGTACGACTTCGCGCTCCACCCCTACGGCTTCGACGAGCTG
GCCACCGACGCGCTGGCCGTCCTGGACGCCTGGCAGGTCCGCGCCGCCCATGTGGTGGGC
ATGTCGCTGGGCAACACCATCGGCCAGTTGCTCGCCCTGGACGCTCCGGAGCGTCTCCTG
ACACTCACCGTCATGCTGGGGGGTGCGCTCGATGTCGACTTCGACGCCGACCTGGAGGCG
GCGCTCAAAGGTGAGCCTTCCGTCAGCGGACTGCCGGTGCCCAGCCGACGGTTCCTCGAC
ATGATGATGCTTCTTCAGCAGCCGGCCGGGACGGACGAGGAACTACTGGAGCGCCGGGTG
GAGAAGTGGCGCCTGCTCAACGGGGAGGGAGTGCCTTTCGACTCCGACGAGTTCCGGCGC
CGTGAACTCCTGGCCGCCGGTCACGCGGGAACGTTCGACGAGCCGATCGTGCACCACATG
ATCCCGCAGCCGCCGGTGTCGCGGGGGGCGGAGTTGGCCCGCATCACGACACCGGTACTG
GCGATCCAGGCGATGTGCGACCCCGCGGCCCCGCCGCCGCACGCCCGGCACCTGGCCGAC
CGAATTCCCGGCGCCCGGGTCGTGGAGATCGAGAACATGGGGCACGCGCTGCCCCTCGCG
GTCCACGAACCGCTGGCCGCCGCCATCTGCGCCCATACCCGGGCCGCGACCGTGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	No significant hit
SignalP	No significant hit
TMHMM	No significant hit

Dauno_00100 Dauno_00120