Acla_00120 : CDS information

Location

Organism	Streptomyces galilaeus
Strain	ATCC 31615
Entry name	Aclacinomycin A
Contig	AF257324
Start / Stop / Direction	11,371 / 10,511 / - [in whole cluster] 11,371 / 10,511 / - [in contig]
Location	complement(10511..11371) [in whole cluster] complement(10511..11371) [in contig]
Type	CDS
Length	861 bp (286 aa)

Annotation

Category	3.2 modification methylation
Product	putative aklanonic acid O-methyltransferase
Product (GenBank)	AknG
Gene
Gene (GenBank)	aknG
EC number	2.1.1.-
Keyword
Note
Note (GenBank)	aklanonic acid methyl transferase
Reference	ACC Q9L546 PmId [12137949] Cloning and characterization of Streptomyces galilaeus aclacinomycins polyketide synthase (PKS) cluster. (Gene. , 2002) comment aclacinomycin PKSを含んだ13.5kb領域のクローニング、シークエンス報告論文。 AknG: methyl transferase 配列相同性から、aklanonic acidのcarboxyl基(-COOH)をエステル化するmethyltransferaseであるとされている。
Related Reference	ACC Q54818 NITE Adria_00310 PmId [7601857] Functional characterization and transcriptional analysis of a gene cluster governing early and late steps in daunorubicin biosynthesis in Streptomyces peucetius. (J Bacteriol. , 1995) comment Blast 6th, id64%, 3e-99 Streptomyces peucetius Putative uncharacterized protein [DoBISCUIT]aklanonic acid O-methyltransferase_dnrC Streptomyces lividans TK24にpWHM918 (carrying dnrC)導入。この株はaklanonic acid → aklanonic acid methylester(AAME)の変換ができる。この変換でできた産物をdpsB(-)mutantに加えると、RHOへ変換可能。よって正しい中間体。これらの演繹は、相同なStreptomyces sp. strain C5 dauC geneの機能と完全に一致する。

Sequence

>putative aklanonic acid O-methyltransferase [AknG]
MDTSTYKSEVAAAFNSAAATYDRLGVEFFTPMGRRLVERAAPAAGARVLDVGCGRGACVF
PAAERVGPEGRVVGIDVAEAMIEEATKEAALRDAAMVELRVMDGEHPDFAPHSFDVVLGS
YSVIFLPDAPTALARYAPLLSPGGRLAFTSPVFSDDTFPFLPPVFTELIPRELLLDLPPQ
WQPEQLQQRFNSWLADPRDLTRTLERCGFQEVTVVDEPVTLVAESGLAWVDWSHTQGMRL
LWNHLAQDKRQQLRERLITSLDAMRDGDGPLTIDTPVRYVTATVRN

>putative aklanonic acid O-methyltransferase [AknG]
GTGGACACGTCCACCTACAAATCCGAAGTGGCGGCCGCCTTCAACAGCGCCGCGGCGACC
TACGACCGGCTCGGCGTGGAATTCTTCACCCCGATGGGCCGGCGTCTGGTGGAACGCGCG
GCACCGGCCGCCGGCGCCCGGGTCCTCGACGTCGGCTGCGGGCGGGGCGCGTGCGTCTTC
CCGGCTGCCGAACGCGTGGGGCCCGAGGGCCGTGTCGTGGGCATCGACGTCGCCGAGGCG
ATGATCGAGGAGGCCACGAAGGAGGCGGCGCTGCGCGACGCCGCCATGGTCGAGCTGCGG
GTGATGGACGGGGAGCACCCCGACTTCGCGCCGCACTCCTTCGACGTGGTCCTGGGCAGC
TACAGCGTCATCTTCCTGCCCGACGCCCCGACGGCGCTGGCCCGTTACGCGCCGCTGCTC
TCCCCCGGGGGCCGACTCGCCTTCACCAGCCCGGTGTTCAGCGACGACACCTTCCCCTTC
CTGCCCCCGGTGTTCACCGAACTGATCCCGCGCGAGCTGCTGCTCGACCTGCCGCCGCAG
TGGCAGCCCGAACAGCTTCAGCAGCGCTTCAACAGCTGGCTCGCGGACCCGCGGGACCTC
ACCCGCACACTGGAGCGGTGCGGCTTCCAAGAGGTGACGGTCGTCGACGAACCCGTGACT
CTGGTGGCCGAATCGGGCCTGGCCTGGGTGGACTGGTCGCACACCCAGGGGATGCGCCTG
CTCTGGAACCACCTGGCGCAGGACAAACGTCAGCAGCTGCGGGAGCGGCTGATCACCTCG
CTCGACGCGATGCGGGACGGGGACGGCCCGCTCACGATCGACACCCCGGTGCGGTACGTC
ACCGCGACCGTACGGAACTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	No significant hit
SignalP	No significant hit
TMHMM	No significant hit

Acla_00110 Acla_00130