Avil_00360 Avil_00360   Avil_00380 Avil_00380

Avil_00370 : CDS information

close this sectionLocation

Organism
StrainTü57
Entry nameAvilamycin
Contig
Start / Stop / Direction35,950 / 36,954 / + [in whole cluster]
35,950 / 36,954 / + [in contig]
Location35950..36954 [in whole cluster]
35950..36954 [in contig]
TypeCDS
Length1,005 bp (334 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productputative 2-oxo acid dehydrogenase E1 component alpha subunit
Product (GenBank)putative pyruvate dehydrogenase
Gene
Gene (GenBank)aviB1
EC number
Keyword
  • eurekanate
  • acetyl group
Note
Note (GenBank)
  • AviB1
Reference
ACC
PmId
[11410376] Biosynthesis of the orthosomycin antibiotic avilamycin A: deductions from the molecular analysis of the avi biosynthetic gene cluster of Streptomyces viridochromogenes Tu57 and production of new antibiotics. (Chem Biol. , 2001)
[15640214] Genes involved in formation and attachment of a two-carbon chain as a component of eurekanate, a branched-chain sugar moiety of avilamycin A. (Appl Environ Microbiol. , 2005)
comment
[PMID:11410376]
Avilamycin生合成遺伝子クラスターの報告。

AviB1: Pyruvate dehydrogenase (alpha chain) from C. magnum [Proposed function:Modification]

AviB1とAviB2でvarious 2-oxo-acid dehydrogenase complexesを形成していると推測。


[PMID:15640214]
AviB1,AviB2,AviO1,AviO2,AviO3の機能解析の報告。

各欠損株を作製し、NMRにて構造解析を行っている。
AviB1,AviB2は相同性や保存配列から、pyruvate dehydrogenaseのheterotetrameric E1 (alpha2beta2) complexであるとしている。
aviB1 mutantで検出したavilamycin誘導体はeurekanateのC-4 positionでacetyl groupを欠いていた。
AviB1,AviB2はpyruvate をacetyl carbanionへ変換する反応を担うタンパクをエンコードすると示した。
Related Reference
ACC
Q46142
PmId
[8206840] ( , )
comment
Clostridium magnum
TPP-dependent acetoin dehydrogenase alpha-subunit_acoA

acoA+acoB plasmidをE.coliへ導入。
acetoin dehydrogenase 活性を示した。

close this sectionSequence

selected fasta
>putative 2-oxo acid dehydrogenase E1 component alpha subunit [putative pyruvate dehydrogenase]
MTTEEGKPAMSAEPRAVRLYRTMRLIREFEERCLAMSLSGEIVGGIHPYIGQEAVAAGVC
AALTDDDVVTSTHRGHGHVLAKGADPKRMLAELCGTTAGLNRGRGGSMHAADVSLGVYGA
NGIVGAGAPIAAGAAWSFQRRGDGRVAVAFFGDGALSQGVVLEAFNLAALWRLPVVFVCE
NNGYATSLPLDRALAGDPVERAAGFGLTARAVDGMDADAVADAAAEAVERCRRGGGPTFL
DCRTYRFNGHHSFEEQVGLNYRDDAEVGEWRARDPLRTQSTRVDGATAGAIDAETSTLID
EAVEFARAGRAPDPAEALHHLYADGTTPRPGVMI
selected fasta
>putative 2-oxo acid dehydrogenase E1 component alpha subunit [putative pyruvate dehydrogenase]
ATGACCACGGAAGAAGGGAAACCGGCGATGAGCGCCGAACCGCGTGCCGTCCGGCTCTAC
CGGACCATGCGCCTGATCAGGGAATTCGAAGAACGCTGCCTGGCGATGTCGCTGTCCGGG
GAGATCGTCGGCGGCATCCACCCCTACATCGGCCAGGAGGCGGTGGCCGCCGGGGTCTGC
GCGGCGCTCACCGACGACGACGTCGTCACCAGCACCCATCGCGGCCATGGCCACGTCCTC
GCCAAGGGAGCCGACCCGAAGCGGATGCTGGCCGAGCTGTGCGGCACGACCGCCGGCCTG
AACCGGGGCCGCGGCGGCTCGATGCACGCCGCCGACGTCAGCCTCGGCGTCTATGGCGCC
AACGGCATCGTGGGGGCGGGCGCCCCCATCGCCGCCGGTGCGGCCTGGTCGTTCCAGCGG
CGCGGTGACGGCCGGGTGGCGGTGGCCTTCTTCGGCGACGGTGCGCTCAGCCAGGGCGTG
GTGCTGGAGGCGTTCAACCTGGCGGCCCTGTGGCGGCTGCCGGTGGTGTTCGTCTGCGAG
AACAACGGCTACGCGACCAGCCTGCCGCTGGACCGGGCGCTGGCCGGCGACCCGGTGGAG
AGGGCGGCCGGGTTCGGCCTGACGGCGCGCGCGGTGGACGGAATGGACGCCGACGCCGTC
GCCGACGCGGCCGCCGAGGCCGTCGAACGCTGCCGCCGGGGCGGCGGTCCCACCTTCCTG
GACTGCCGCACCTACCGGTTCAACGGCCACCACTCCTTCGAGGAGCAGGTGGGGCTGAAC
TACCGGGACGACGCCGAGGTCGGCGAGTGGCGTGCGCGTGACCCGCTGCGCACCCAGAGC
ACCCGCGTGGACGGTGCGACGGCCGGCGCCATCGACGCCGAGACCAGCACGCTGATCGAC
GAGGCCGTGGAGTTCGCCCGCGCGGGCCGGGCCCCCGACCCCGCCGAGGCGCTGCACCAC
CTTTATGCCGACGGCACGACCCCACGGCCCGGAGTGATGATCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001017 Dehydrogenase, E1 component (Domain)
[22-306] 2.90000000000003e-70 PF00676
PF00676   E1_dh
SignalP No significant hit
TMHMM No significant hit
Avil_00360 Avil_00360   Avil_00380 Avil_00380
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