Avil_00380 : CDS information

Location

Organism	Streptomyces viridochromogenes
Strain	Tü57
Entry name	Avilamycin
Contig	AF333038
Start / Stop / Direction	36,954 / 37,916 / + [in whole cluster] 36,954 / 37,916 / + [in contig]
Location	36954..37916 [in whole cluster] 36954..37916 [in contig]
Type	CDS
Length	963 bp (320 aa)

Annotation

Category	3.3 modification reduction
Product	putative 2-oxo acid dehydrogenase E1 component beta subunit
Product (GenBank)	putative pyruvate dehydrogenase
Gene
Gene (GenBank)	aviB2
EC number
Keyword	eurekanate acetyl group
Note
Note (GenBank)	AviB2
Reference	ACC Q93KV4 PmId [11410376] Biosynthesis of the orthosomycin antibiotic avilamycin A: deductions from the molecular analysis of the avi biosynthetic gene cluster of Streptomyces viridochromogenes Tu57 and production of new antibiotics. (Chem Biol. , 2001) [15640214] Genes involved in formation and attachment of a two-carbon chain as a component of eurekanate, a branched-chain sugar moiety of avilamycin A. (Appl Environ Microbiol. , 2005) comment [PMID:11410376] Avilamycin生合成遺伝子クラスターの報告。 AviB2: Pyruvate dehydrogenase (beta chain) from C. magnum [Proposed function:Modification] AviB1とAviB2でvarious 2-oxo-acid dehydrogenase complexesを形成していると推測。 [PMID:15640214] AviB1,AviB2,AviO1,AviO2,AviO3の機能解析の報告。各欠損株を作製し、NMRにて構造解析を行っている。 AviB1,AviB2は相同性や保存配列から、pyruvate dehydrogenaseのheterotetrameric E1 (alpha2beta2) complexであるとしている。 aviB1 mutantで検出したavilamycin誘導体はeurekanateのC-4 positionでacetyl groupを欠いていた。 AviB1,AviB2はpyruvate をacetyl carbanionへ変換する反応を担うタンパクをエンコードすると示した。
Related Reference	ACC Q46143 PmId [8206840] ( , ) comment Clostridium magnum TPP-dependent acetoin dehydrogenase beta-subunit_acoB acoA+acoB plasmidをE.coliへ導入。 acetoin dehydrogenase 活性を示した。

Sequence

>putative 2-oxo acid dehydrogenase E1 component beta subunit [putative pyruvate dehydrogenase]
MAALAYITALNQALHDEMARDDRVCVFGEDVRIGLTQVAKGLHERFGDGRVVDTPLSEQA
FTSLATGAAMAGQRPVVEYQIPSLLYLVFEQIANQAHKFSLMTGGQVEVPVTYLVPGSGS
RSGMAGQHSDHPYSLFAHVGIKTVLPATASDAYGLLLSAVRDPDPVAVFAPSALMGTVEE
VSGELGPVPLGSARIHRTGEDVTVVATGQCVHVALAVAEAMADEASIEVVDPRTIYPVDW
ETIRASAEKTGRLVVIDDANRMCGFGGEVLATAAEQFDLTARPRRVTRPDGAVIPYALVL
DQALLPDAAQLTDAIRAVLK

>putative 2-oxo acid dehydrogenase E1 component beta subunit [putative pyruvate dehydrogenase]
ATGGCTGCACTTGCGTACATCACCGCGCTGAACCAGGCGCTGCACGACGAGATGGCGCGC
GACGACCGCGTCTGCGTCTTCGGCGAGGACGTGCGGATCGGCCTCACCCAGGTCGCCAAG
GGCCTGCACGAGCGCTTCGGCGACGGACGCGTCGTCGACACCCCCCTGTCGGAGCAGGCC
TTCACCAGCCTGGCCACCGGCGCGGCGATGGCGGGGCAGCGACCGGTCGTGGAGTACCAG
ATCCCCTCGCTGCTCTATCTGGTGTTCGAGCAGATCGCCAACCAGGCGCACAAGTTCTCG
CTGATGACCGGTGGGCAGGTGGAGGTCCCGGTCACCTATCTGGTGCCGGGCTCCGGTTCG
CGCTCGGGCATGGCCGGCCAGCACTCCGACCATCCCTACAGCCTGTTCGCACACGTCGGC
ATCAAGACGGTCCTGCCCGCCACCGCCTCGGACGCCTACGGCCTGCTGCTCTCGGCGGTC
CGTGACCCGGACCCGGTGGCCGTCTTCGCGCCGAGCGCGCTGATGGGCACGGTCGAGGAG
GTCTCCGGCGAGCTCGGCCCGGTGCCGCTCGGGTCGGCCCGGATCCACCGCACGGGCGAG
GACGTCACCGTCGTCGCCACCGGCCAGTGCGTGCATGTCGCCCTGGCCGTCGCGGAGGCC
ATGGCGGACGAGGCGTCGATCGAGGTGGTGGATCCGCGCACGATCTACCCGGTGGACTGG
GAGACGATCCGGGCGTCGGCGGAGAAGACCGGACGGCTGGTCGTCATCGATGACGCCAAC
CGGATGTGCGGGTTCGGCGGCGAGGTGCTGGCCACCGCGGCGGAGCAGTTCGATCTGACG
GCCCGGCCCCGGCGGGTGACCCGGCCGGACGGCGCGGTCATCCCCTACGCGCTCGTGCTC
GACCAGGCGTTGCTGCCGGACGCGGCCCAGCTGACCGACGCGATCCGTGCCGTCCTGAAG
TGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005475 Transketolase-like, pyrimidine-binding domain (Domain) SM00861 Transket_pyr PF02779 Transket_pyr IPR005476 Transketolase, C-terminal (Domain) PF02780 Transketolase_C IPR009014 Transketolase, C-terminal/Pyruvate-ferredoxin oxidoreductase, domain II (Domain) SSF52922 Transketo_C_like IPR015941 Transketolase-like, C-terminal (Domain) G3DSA:3.40.50.920 Transketo_C_like
SignalP	No significant hit
TMHMM	No significant hit

Avil_00370 Avil_00390