Avil_00390 Avil_00390   Avil_00410 Avil_00410

Avil_00400 : CDS information

close this sectionLocation

Organism
StrainTü57
Entry nameAvilamycin
Contig
Start / Stop / Direction38,971 / 39,999 / + [in whole cluster]
38,971 / 39,999 / + [in contig]
Location38971..39999 [in whole cluster]
38971..39999 [in contig]
TypeCDS
Length1,029 bp (342 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productglycosyltransferase
Product (GenBank)putative glycosyltransferase
Gene
Gene (GenBank)aviGT4
EC number
Keyword
  • eurekanate
Note
Note (GenBank)
  • AviGT4
Reference
ACC
PmId
[11410376] Biosynthesis of the orthosomycin antibiotic avilamycin A: deductions from the molecular analysis of the avi biosynthetic gene cluster of Streptomyces viridochromogenes Tu57 and production of new antibiotics. (Chem Biol. , 2001)
[16242656] Genes encoding enzymes responsible for biosynthesis of L-lyxose and attachment of eurekanate during avilamycin biosynthesis. (Chem Biol. , 2005)
[17113996] Insights into the synthesis of lipopolysaccharide and antibiotics through the structures of two retaining glycosyltransferases from family GT4. (Chem Biol. , 2006)
comment
[PMID:11410376]
Avilamycin生合成遺伝子クラスターの報告。

AviGT4:Putative mannosyltransferase (WbaZ-2) from A. fulgidus [Proposed function:Biosynthesis of the heptasaccharide chain]

このORFについて機能解析はされていない。


[PMID:16242656]
AviGT4の機能解析の報告。

AviGT4 の不活性化株はterminal eurekanate residueが欠損した新規avilamycin誘導体を合成し、AviGT4はL-lyxose moietyへのeurekanate residueのtransferに関与すると示した。しかし、AviGT4がsimple glycosidic bondを形成するのかあるいはorthoesterの全体の形成を触媒するのかはわかっていない。
また、AviGT4の不活性化実験からdisaccharide L-lyxose-eurekanateがavilamycin Aのスターター分子ではないことを示し、avilamycin Aの生合成は1→1 linked disaccharide D-mannopyranosyl-L-lyxoseの形成により始まると推測している。


[PMID:17113996]
Glycosyltransferases Family GT4の結晶構造解析の報告。

close this sectionSequence

selected fasta
>glycosyltransferase [putative glycosyltransferase]
MRPLKVALVNIPLRVPGSDAWISVPPQGYGGIQWVVANLMDGLLELGHEVFLLGAPGSPA
GRPGLTVVPAGEPEEIERWLRTADVDVVHDHSGGVIGPAGLPPGTAFISSHHFTTRPVNP
VGCTYSSRAQRAHCGGGDDAPVIPIPVDPARYRSAADQVAKEDFLLFMGRVSPHKGALEA
AAFAHACGRRLVLAGPAWEPEYFDEITRRYGSTVEPIGEVGGERRLDLLASAHAVLAMSQ
AVTGPWGGIWCEPGATVVSEAAVSGTPVVGTGNGCLAEIVPSVGEVVGYGTDFAPDEARR
TLAGLPASDEVRRAAVRLWGHVTIAERYVEQYRRLLAGATWK
selected fasta
>glycosyltransferase [putative glycosyltransferase]
ATGAGGCCGTTGAAGGTGGCGCTGGTCAACATCCCGCTGCGGGTCCCGGGGAGCGATGCC
TGGATCTCCGTACCCCCACAGGGATACGGCGGCATCCAGTGGGTCGTCGCCAACCTGATG
GACGGGCTGCTGGAACTCGGCCACGAGGTCTTCCTGCTGGGTGCCCCGGGCAGCCCGGCC
GGACGCCCGGGGCTGACCGTCGTGCCGGCGGGCGAGCCGGAGGAGATCGAGCGGTGGCTG
CGGACCGCGGACGTCGACGTGGTCCACGACCACAGCGGCGGTGTCATCGGCCCGGCCGGG
CTGCCGCCGGGCACCGCCTTCATCAGCTCGCACCACTTCACCACCCGGCCGGTCAACCCC
GTGGGCTGTACCTACAGTTCCCGGGCGCAGCGCGCCCACTGCGGGGGCGGCGACGACGCG
CCCGTGATCCCGATCCCGGTCGACCCGGCGCGCTACCGGTCCGCCGCGGACCAGGTGGCC
AAGGAGGACTTCCTGCTCTTCATGGGGCGGGTCTCGCCGCACAAGGGGGCGCTGGAGGCG
GCCGCGTTCGCGCACGCCTGCGGCCGGCGCCTGGTGCTGGCCGGGCCCGCCTGGGAGCCG
GAGTACTTCGACGAGATCACTCGCCGGTACGGCTCGACCGTCGAGCCGATCGGTGAGGTG
GGCGGCGAGCGGCGGCTCGACCTGCTCGCCTCCGCACACGCGGTGCTGGCCATGTCCCAG
GCGGTCACCGGGCCGTGGGGCGGCATCTGGTGCGAGCCGGGTGCGACGGTGGTCTCCGAG
GCCGCCGTGAGCGGCACTCCCGTCGTGGGCACGGGCAACGGCTGCCTGGCCGAGATCGTG
CCCTCGGTCGGCGAAGTCGTGGGATACGGCACCGACTTCGCGCCCGACGAAGCACGCCGG
ACCCTGGCAGGGCTGCCGGCATCCGACGAGGTCCGGCGTGCGGCGGTCCGGTTGTGGGGC
CATGTGACGATCGCCGAACGGTATGTGGAGCAGTACCGCAGGCTGCTGGCCGGAGCGACC
TGGAAGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001296 Glycosyl transferase, family 1 (Domain)
[159-282] 3.9e-07 PF00534
PF00534   Glycos_transf_1
SignalP
[1-20] 0.408 Signal
Eukaryota   
TMHMM No significant hit
Avil_00390 Avil_00390   Avil_00410 Avil_00410
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