Avil_00500 : CDS information

Location

Organism	Streptomyces viridochromogenes
Strain	Tü57
Entry name	Avilamycin
Contig	AF333038
Start / Stop / Direction	49,961 / 50,938 / + [in whole cluster] 49,961 / 50,938 / + [in contig]
Location	49961..50938 [in whole cluster] 49961..50938 [in contig]
Type	CDS
Length	978 bp (325 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative NDP-hexose C-4 ketoreductase
Product (GenBank)
Gene	aviZ3
Gene (GenBank)
EC number
Keyword	D-olivose 2-deoxy-D-evalose
Note
Note (GenBank)
Reference	PmId [11410376] Biosynthesis of the orthosomycin antibiotic avilamycin A: deductions from the molecular analysis of the avi biosynthetic gene cluster of Streptomyces viridochromogenes Tu57 and production of new antibiotics. (Chem Biol. , 2001) comment avilamycin A生合成遺伝子クラスターの報告。 AviZ3:Putative 4-ketoreductase (SnoG) from S. nogalater [function:Sugar biosynthesis (D-olivose, 2-deoxy-D-evalose)] AviS,AviT,AviZ3はNDP-4-keto-6-deoxy-D-glucose→NDP-D-olivoseの反応に関与すると推測している。このORFの機能解析はされていない。
Related Reference	ACC O33708 NITE Adria_00200 PmId [9209071] Cloning and characterization of the Streptomyces peucetius dnmZUV genes encoding three enzymes required for biosynthesis of the daunorubicin precursor thymidine diphospho-L-daunosamine. (J Bacteriol. , 1997) [10631513] A two-plasmid system for the glycosylation of polyketide antibiotics: bioconversion of epsilon-rhodomycinone to rhodomycin D. (Chem Biol. , 1999) [9447597] Production of the antitumor drug epirubicin (4'-epidoxorubicin) and its precursor by a genetically engineered strain of Streptomyces peucetius. (Nat Biotechnol. , 1998) comment Streptomyces peucetius Thymidine diphospho-4-keto-2,3,6-trideoxyhexulose reductase_dnmV Daunorubicin生合成遺伝子 [PMID: 9209071] dTDP-L-daunosamineの生合成に必要なdnmZUV genesのクローニングと特徴づけ。 dnmV: 4-keto基の還元 dnmV不活化mutant産物解析と相補による回復から、daunosamine生合成に関与することは確認されている。dTDP-4-ketodeoxyhexulose ketoreductase機能の割り当ては配列解析に基づく。 --- [PMID: 10631513] 異種性ホストにおけるL-daunosamineの生合成と付着のための最少の情報は、dnmLMJVUTS genesによってコードされる。 DnmV とavrE and eryBIV genesの産物は、DNR and DXRに由来する4'-epimeric anthracyclinesの合成を通して、dTDP-4-ketohexulose reductasesであることが証明されている(ref26)。 ↓そのref26 [PMID: 9447597] S.peucetius dnmV mutantに、Streptomyces avermitilis avrE or Saccharopolyspora eryBIV genesを異種性に導入すると、epirubicin (4'-epidoxorubicin) and 4'-epidaunorubicinを形成。 Saccharopolyspora eryBIV = dTDP-4-keto-6-deoxy-L-hexose 4-reductase

Sequence

>putative NDP-hexose C-4 ketoreductase
MADERERVLVAGGTGFVGRQLCADLTAAGAEVAAIARRVPDFPLPGRLLVLDVTTASPGE
LADVIDSVRPHTVVNAIGSNWGIAERDMETNCAVPTRRLLDALRRTACRPYVVHLGSVLE
YGPTPPGEPTRTASPPRPTTAYGKAKLAASQAVLEAAAEGVVEAGVLRIANVAGPGTPAV
SLLGRVAGRLAEAVTRDMLPAVVELSQLRAHRDYVDVRDVSDAVLAATRARIPGLVVPIG
RGEAVPVRWLVDLLVEVSGVPAEVRELPAAATGAAGDDWIQVDPGPARELLGWTAVRSLH
ESVTGLWADTLRRQAIPQHAEPRPS

>putative NDP-hexose C-4 ketoreductase
ATGGCCGATGAGCGCGAGCGCGTTCTCGTGGCTGGTGGGACGGGGTTCGTGGGCCGTCAG
CTGTGCGCGGACCTGACCGCGGCCGGAGCCGAGGTGGCGGCGATCGCCCGGCGGGTACCG
GACTTCCCCCTGCCGGGCCGGCTCCTCGTCCTGGACGTCACCACCGCGTCGCCCGGGGAA
CTGGCCGACGTGATCGACTCCGTCCGCCCGCACACGGTGGTCAACGCCATCGGCAGCAAC
TGGGGGATCGCCGAACGGGACATGGAGACCAACTGCGCGGTGCCGACCCGCCGCCTGCTG
GACGCGCTGCGGCGGACCGCGTGCCGTCCCTACGTCGTCCACCTCGGCTCAGTGCTGGAG
TACGGCCCGACCCCGCCCGGTGAACCGACCCGGACCGCGTCGCCGCCCCGGCCGACGACC
GCCTACGGCAAGGCCAAACTCGCGGCGAGCCAGGCCGTGCTGGAGGCCGCCGCGGAGGGC
GTCGTGGAGGCGGGCGTGCTGCGGATCGCCAACGTGGCCGGGCCGGGCACCCCGGCGGTC
AGCCTGCTCGGCCGGGTGGCCGGGCGGCTCGCCGAGGCGGTCACCCGGGACATGCTCCCG
GCGGTCGTGGAGCTGTCCCAGTTGCGCGCCCACCGCGACTACGTCGACGTGCGCGACGTC
TCGGACGCGGTGCTGGCCGCCACCCGCGCCCGTATTCCGGGGCTGGTCGTCCCCATCGGA
CGCGGCGAGGCCGTGCCGGTGCGGTGGCTCGTCGATCTGCTCGTCGAGGTGAGCGGCGTG
CCCGCCGAGGTCCGGGAGCTTCCCGCCGCCGCCACGGGCGCGGCCGGGGACGACTGGATC
CAGGTCGACCCCGGGCCCGCCCGCGAACTCCTCGGCTGGACCGCCGTCCGCTCGCTGCAC
GAGTCGGTGACCGGGCTGTGGGCCGACACGCTGCGCAGGCAGGCAATCCCCCAGCACGCG
GAGCCGCGGCCGTCCTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001509 NAD-dependent epimerase/dehydratase (Domain) PF01370 Epimerase IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 no description
SignalP	No significant hit
TMHMM	No significant hit

Avil_00490 Avil_00510