Chalc_00210 Chalc_00210   Chalc_00230 Chalc_00230

Chalc_00220 : CDS information

close this sectionLocation

Organism
StrainNRRL 2737
Entry nameChalcomycin
Contig
Start / Stop / Direction37,041 / 42,965 / + [in whole cluster]
37,041 / 42,965 / + [in contig]
Location37041..42965 [in whole cluster]
37041..42965 [in contig]
TypeCDS
Length5,925 bp (1,974 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase
Product (GenBank)polyketide synthase subunit
Gene
Gene (GenBank)chmGII
EC number
Keyword
Note
Note (GenBank)
  • module 3 KS/AT/DH/KR/ACP
Reference
ACC
PmId
[15561847] Chalcomycin biosynthesis gene cluster from Streptomyces bikiniensis: novel features of an unusual ketolide produced through expression of the chm polyketide synthase in Streptomyces fradiae. (Antimicrob Agents Chemother. , 2004)
[15489173] Production of hybrid 16-membered macrolides by expressing combinations of polyketide synthase genes in engineered Streptomyces fradiae hosts. (Chem Biol. , 2004)
comment
[PMID:15561847](2004)
Chalcomycin biosynthesis gene clusterに関する文献。

chmGII(1,974a.a.): PKS, module 3

tyl PKS genesを削除したS.fradiae K159-1でchm PKS genesを発現すると、5-O-mycaminosylchalcolactone産生。よって、これらgenesはchalcomycin PKSであり、同定したclusterはchalcomycin生合成をする。

--
[PMID:15489173](2004)
hybrid PKS作成にchmGI, GIIが使用されている。

close this sectionPKS/NRPS Module

3 malonyl-CoA
KS35..407
AT566..913
DH961..1141
KR1488..1668
ACP1803..1873

close this sectionSequence

selected fasta
>polyketide synthase [polyketide synthase subunit]
MTANDDKIRDYLKRVVAELHSTRQRLNALEHDAREPIAIVGMSCRLPGGVTTPESLWRLV
DSGTDAASPFPDDRGWDLDALHHPESGAVHSREGGFLHDSADFDAEFFGISPREALAMDP
QQRLLLETAWEVFERAGIDPVSARGSRTGVYAGVMYHDYGARLNEIPPGLEGYLVNGSAG
SIASGRVAYTLGLEGPAVTVDTACSSSLVAVHLAAQALRRRECDMALAGGATVLSTPDLF
IDFARLGGLASDGRCKAFSDAADGTSFAEGAGLLLLMRLSDAVAEGHTVLAVVRGSAVNQ
DGASNGLTAPNGLAQQRVIREALADADLDPDQIDAVEAHGTGTRLGDPIEAQALLHTYGT
SRSPERPLWLGSLKSNIGHTQAAAGVAGVIKTVLAMRHGRLPRTLHVTRPSSRVEWSAGA
VELLTRAQDWPGQGNAPRRAGVSSFGASGTNAHLILEGVPDGDITVAETRPATGGGAWPL
AGRTEAALRAQARRLHDHLAARPHVSPAAVGRTLVRSRTAFEHRAVVLGQDTADLLSGLA
ELASGGAHGPGVITGRAARGRRTALLFTGQGSQRPGAGRHLYERYEVFARALDETAAALD
RHLDRPLRDVMFAEPGGATAGLLDRTEYTQPALFALEVALFRLVTAGGLRPDALLGHSVG
ELAAAHVAGVFTLPDAARLVTARGRLMGELPAGGAMMAIQASGPEIEETITALAAHRSAR
VAVAALNGPDATVISGDEDVVAELATLWRERGRRTRALPVSHAFHSPHMDAALEPFARIA
RDVSYAEPRIPVVSNLTGGIASATTLCAPEYWVRHAREAVRFSDGFRALRDQGIDTFIEL
GPDGVLSALGRDCLREEEGDAPRQDGSADPDTTGSRADGGRRPVLTVPLLRRDRDETTTC
LGALATVHTHGVPVDLAAVHGAPEGPAVELPTYAFQRTRYWLDAPAPAAGPTATGLEATD
QPLLPAVIDLPDGEGTVRTGLLSLRTHPWIADHRVRDHAVVPGAALLDVAAWAGTEAGCP
RVAELTFATPLVLPENGEGVRLRVTVSGPDAEGIRSLRIDSRPADTVRTADAPSDWTRHA
SGTLVPAPEEAGDGTGVPTELLGAWPPADATPVALDADAVAAEYQRLAAGGVTYGPAFRA
LRAVWRRGAEVFAEVRLPGQAAADASRYGMHPALLDALTHATGFGERSTEARGLVPFAWS
DVRIHVRGADSLRVRIAPAGPDAVTVAAVDPTGRPVLAARSLTLRPLAESRFQDPEADST
PLYRLEWTPAPGSVTGHAGPRQAAAEWGVLGDPVQALLDAVRDGAEAPVRTHDDLLALAA
SDTAPPDHVLALLGHDGDALATGAHDLAARALALVQGWLTHARFADARLVVLTQGAVTAG
TSPVHPAAAAAWGLLRSAQSEHPGRFVLVDADPADPAASYRSLPRAVASGASQLALRGAE
ILVPRLARGTDRQATVPGHPGDVTAPETTAAPEPAPSGTPSGPWPADGTVLVTGGTGTLG
KAVARHLVTKHGVRHLILAGRRGADTPGAADLATELTGLGATVNIVRCDAADRSALEGVL
AAVPAAHPLTAVVHTAGVLDDGIVTAQTPRRLSAVLRAKADAVSHLHELTRDLDLSAFVL
FSSAAGTLGSPGQSGYAAANSFLDAFAAWRRAQGLPAVSLAWGLWGDGGDGRDGGGSAAD
GMGASLAAADLARLRRSGILPLDPAEALRLFDEACDPARTEAVLLPIRLDLTGLRARSAR
GAVHASVVPEVLHTLVPPPAGAGSPAGADASDPAAGQAPPAPASDTLAERLAGKPRGERL
TALTELVRTEIASVLGHPDSGRVQLQSSFKESGFDSLTAVELRNRLTAATGTKLPATLVF
DHPTPAALVDHLEQELPKAAQEIPADLPAVLDALDRIRDGLATAATDDSSRDHIAERLQA
LLGTLTSAAGVSRPTGSPGEHDRQGPDELSLGQRLAASSDDELFDLFDSDFRST
selected fasta
>polyketide synthase [polyketide synthase subunit]
ATGACGGCCAACGATGACAAGATCCGCGACTACCTGAAGCGGGTCGTCGCCGAGCTGCAC
AGCACGCGGCAACGGCTCAACGCCTTGGAGCACGACGCCCGCGAGCCCATCGCCATCGTG
GGGATGAGCTGCCGGCTGCCCGGCGGGGTGACCACCCCCGAGAGCCTGTGGAGGCTGGTC
GACTCCGGCACCGACGCCGCCTCGCCGTTCCCCGACGACCGGGGCTGGGACCTGGACGCG
CTCCACCATCCGGAGTCGGGAGCCGTCCACTCCCGCGAGGGCGGATTCCTCCACGACAGC
GCGGACTTCGACGCGGAGTTCTTCGGCATCAGCCCGCGAGAGGCCCTGGCCATGGACCCG
CAGCAGCGGCTGCTGCTGGAGACCGCCTGGGAGGTGTTCGAGCGCGCCGGCATCGACCCG
GTCTCCGCCCGCGGCTCCCGCACGGGGGTGTACGCGGGCGTCATGTACCACGACTACGGC
GCCCGGCTGAACGAGATCCCGCCGGGCCTCGAGGGCTACCTGGTCAACGGCAGCGCGGGC
AGCATCGCCTCGGGCCGGGTGGCCTACACCCTCGGTCTGGAGGGCCCCGCCGTCACCGTC
GACACGGCCTGCTCCTCGTCACTGGTCGCCGTGCACCTGGCGGCACAGGCACTGCGGCGG
CGGGAGTGCGACATGGCGCTCGCGGGCGGCGCGACCGTCCTGTCCACCCCCGACCTGTTC
ATCGACTTCGCGCGACTCGGCGGCCTGGCCTCCGACGGGCGCTGCAAGGCGTTCTCCGAC
GCCGCCGACGGCACCAGCTTCGCCGAGGGCGCCGGCCTGCTGCTCCTCATGCGGCTGTCG
GACGCGGTGGCCGAGGGCCACACCGTCCTGGCGGTCGTCCGAGGCTCCGCCGTCAACCAG
GACGGGGCGAGCAACGGCCTGACGGCCCCCAACGGCCTCGCCCAGCAACGCGTGATCCGC
GAGGCGCTCGCCGACGCGGACCTGGACCCCGACCAGATCGACGCGGTGGAGGCGCACGGC
ACCGGAACCAGGCTCGGCGACCCCATCGAGGCGCAGGCCCTGCTGCACACGTACGGCACG
AGCCGCAGCCCCGAACGACCCCTGTGGCTCGGTTCGCTGAAGTCCAACATCGGCCACACC
CAGGCCGCCGCCGGAGTGGCCGGAGTCATCAAGACGGTGCTGGCGATGCGCCACGGACGG
CTGCCCCGCACACTGCACGTCACCCGCCCCTCCAGCCGGGTGGAATGGTCGGCGGGCGCG
GTGGAACTGCTCACGCGGGCACAGGACTGGCCCGGCCAGGGGAACGCCCCGCGCCGCGCC
GGAGTGTCGTCCTTCGGTGCCAGCGGCACCAACGCACACCTGATCCTGGAAGGCGTCCCG
GACGGCGACATCACGGTCGCGGAGACCCGACCGGCCACCGGCGGCGGCGCCTGGCCGCTC
GCGGGCCGGACCGAAGCGGCCCTGCGCGCACAGGCCCGGCGGCTCCACGACCACCTCGCC
GCCCGCCCCCACGTCTCACCCGCCGCGGTCGGGCGGACGCTGGTCCGCTCCCGCACGGCG
TTCGAGCACCGGGCCGTCGTCCTCGGACAGGACACCGCCGACCTCCTGAGCGGCCTCGCG
GAGCTGGCGTCCGGCGGCGCTCACGGACCCGGCGTGATCACCGGCCGGGCCGCGCGCGGC
CGCCGTACCGCACTGCTCTTCACCGGACAGGGCAGCCAGCGGCCGGGTGCCGGACGGCAT
CTCTACGAGCGGTACGAGGTGTTCGCCCGCGCCCTGGACGAGACGGCCGCGGCACTCGAC
CGGCACCTCGACCGCCCGCTGCGCGACGTGATGTTCGCGGAGCCGGGCGGCGCGACGGCC
GGACTCCTCGACCGCACCGAGTACACCCAGCCCGCACTGTTCGCCCTGGAAGTCGCCCTC
TTCCGACTGGTGACCGCCGGGGGCCTGCGCCCCGACGCACTCCTCGGGCACTCCGTCGGC
GAACTGGCCGCCGCCCACGTCGCCGGAGTGTTCACCCTGCCCGACGCCGCCCGCCTGGTG
ACGGCGCGAGGCCGACTGATGGGCGAGCTGCCGGCCGGTGGCGCCATGATGGCGATCCAG
GCGAGCGGCCCGGAGATCGAGGAGACGATCACGGCGCTCGCGGCCCACCGGTCGGCGCGC
GTCGCCGTCGCCGCACTCAACGGTCCCGACGCCACCGTGATCTCGGGCGACGAGGACGTG
GTCGCCGAACTCGCCACGCTGTGGCGGGAGCGGGGCCGCCGCACCAGGGCGCTGCCCGTC
AGCCACGCCTTCCACTCGCCGCACATGGACGCCGCACTGGAACCGTTCGCCCGGATCGCG
CGCGACGTCTCCTACGCCGAACCGCGCATCCCGGTGGTCTCCAACCTGACCGGCGGCATC
GCGTCGGCCACGACGCTGTGCGCCCCCGAGTACTGGGTGCGCCACGCGCGCGAGGCCGTG
CGCTTCAGCGACGGCTTCCGCGCCCTGCGCGACCAGGGGATCGACACCTTCATCGAGCTC
GGACCGGACGGCGTGCTGTCCGCCCTGGGCCGCGACTGCCTCCGCGAGGAGGAAGGAGAC
GCCCCACGCCAGGACGGCTCGGCGGACCCCGACACGACCGGCTCCCGCGCCGACGGGGGG
CGGCGACCCGTGCTGACGGTGCCGCTGCTGCGCCGGGACCGCGACGAGACGACCACCTGC
CTCGGGGCCCTGGCCACCGTCCACACCCACGGCGTCCCCGTCGACCTCGCGGCCGTGCAC
GGCGCCCCCGAGGGGCCCGCCGTCGAGCTCCCCACCTACGCCTTCCAACGCACGCGCTAC
TGGCTGGACGCCCCGGCCCCCGCCGCCGGCCCCACCGCCACCGGTCTGGAGGCGACGGAC
CAGCCCCTGCTCCCGGCCGTCATCGACCTGCCCGACGGGGAAGGCACCGTACGGACCGGA
CTGCTCTCCCTGCGCACCCATCCGTGGATCGCCGACCACCGCGTCCGCGACCACGCCGTG
GTGCCCGGAGCGGCCCTGCTGGACGTCGCCGCCTGGGCGGGCACCGAAGCGGGCTGCCCC
CGGGTCGCCGAACTGACCTTCGCCACGCCCCTCGTCCTGCCCGAGAACGGAGAAGGAGTC
CGACTGCGCGTGACGGTCAGCGGCCCCGACGCGGAAGGCATCCGTTCGCTACGCATCGAC
TCCCGGCCCGCCGACACGGTCCGTACCGCCGATGCCCCCTCCGACTGGACCCGCCACGCG
TCCGGCACCCTCGTCCCCGCACCCGAGGAGGCCGGCGACGGCACCGGCGTGCCGACCGAA
CTGCTCGGCGCCTGGCCCCCGGCCGACGCGACCCCGGTCGCCCTCGACGCGGACGCCGTC
GCGGCCGAGTATCAGCGCCTCGCGGCCGGCGGCGTGACGTACGGCCCCGCGTTCCGGGCA
CTGCGCGCCGTCTGGCGCCGCGGCGCAGAGGTCTTCGCCGAGGTCCGGCTTCCCGGCCAG
GCGGCCGCCGACGCCTCGCGGTACGGCATGCACCCGGCCCTGCTGGACGCCCTGACGCAC
GCCACCGGGTTCGGCGAGCGGTCCACCGAGGCCCGCGGCCTGGTCCCGTTCGCCTGGAGT
GACGTCCGGATCCACGTCCGCGGCGCCGACTCCCTGCGCGTACGCATCGCGCCGGCCGGC
CCCGACGCCGTGACCGTCGCCGCGGTCGACCCGACGGGCCGCCCGGTCCTCGCCGCCCGC
TCGCTCACGCTGCGCCCCCTGGCGGAGAGCCGGTTCCAGGACCCGGAGGCGGACAGCACG
CCGCTGTACCGACTGGAGTGGACACCGGCCCCCGGTTCCGTGACCGGGCACGCCGGTCCA
AGGCAGGCGGCAGCGGAGTGGGGCGTCCTCGGCGACCCGGTCCAGGCCCTCCTCGACGCC
GTGCGCGACGGGGCGGAGGCACCCGTGCGAACCCATGACGACCTGCTCGCGCTCGCGGCC
TCCGACACGGCCCCGCCCGACCATGTGCTGGCGCTGCTGGGCCACGACGGGGACGCTCTC
GCCACGGGCGCCCACGACCTGGCCGCACGCGCCCTGGCCCTGGTACAGGGCTGGCTGACC
CACGCCCGCTTCGCCGACGCGCGACTGGTCGTGCTGACGCAGGGGGCGGTGACGGCCGGC
ACGAGCCCCGTCCACCCGGCCGCGGCCGCCGCCTGGGGGCTGCTGCGCAGCGCACAGTCC
GAGCACCCGGGCCGCTTCGTCCTCGTCGACGCGGACCCCGCCGACCCGGCCGCCTCGTAC
CGTTCCCTGCCACGGGCCGTCGCCTCCGGGGCGTCCCAACTCGCCCTGCGCGGCGCCGAG
ATCCTCGTCCCCCGGCTCGCCCGGGGAACGGACCGACAGGCCACCGTGCCCGGACACCCC
GGCGACGTCACCGCACCGGAGACGACCGCTGCCCCCGAGCCGGCCCCGTCCGGCACCCCC
TCCGGCCCCTGGCCCGCGGACGGCACCGTGCTCGTCACGGGCGGAACCGGGACCCTGGGC
AAGGCCGTGGCCCGGCACCTCGTGACCAAGCACGGTGTCCGGCACCTGATCCTGGCCGGT
CGGCGAGGCGCGGACACCCCCGGGGCGGCCGACCTGGCCACCGAACTGACCGGCCTGGGC
GCCACCGTGAACATCGTCCGCTGCGATGCCGCCGACCGCTCGGCGCTCGAAGGCGTCCTG
GCCGCCGTCCCCGCCGCGCACCCGCTCACCGCCGTCGTGCACACCGCCGGAGTGCTCGAC
GACGGCATCGTCACGGCGCAGACGCCCCGACGCCTCTCGGCGGTCCTGCGCGCCAAGGCG
GACGCGGTCAGCCACCTGCACGAACTGACCCGCGACCTGGACCTGTCCGCCTTCGTCCTC
TTCTCATCGGCCGCCGGAACCCTCGGCAGCCCCGGCCAGTCCGGCTACGCGGCCGCCAAC
AGCTTCCTCGACGCGTTCGCCGCCTGGCGGCGAGCGCAGGGCCTCCCCGCCGTGTCCCTG
GCATGGGGACTGTGGGGTGACGGCGGTGACGGTCGTGACGGCGGTGGCTCGGCGGCCGAC
GGCATGGGAGCGAGCCTGGCCGCCGCCGACCTGGCACGGCTGCGCCGTTCCGGCATCCTC
CCGCTCGACCCGGCCGAAGCGCTGCGCCTGTTCGACGAGGCCTGCGACCCCGCCAGGACC
GAGGCCGTACTGCTGCCGATCCGCCTCGACCTGACCGGCCTGCGCGCCCGTTCCGCCCGC
GGCGCCGTACACGCGAGCGTGGTGCCCGAAGTGCTGCACACCTTGGTGCCCCCGCCCGCC
GGTGCCGGATCCCCGGCCGGTGCCGACGCGTCGGATCCCGCGGCGGGCCAGGCGCCCCCG
GCCCCGGCGTCCGACACCCTGGCCGAACGGCTCGCCGGGAAGCCCCGAGGCGAACGGCTC
ACCGCCCTCACCGAACTGGTACGCACCGAGATCGCCTCGGTACTCGGGCACCCCGACTCC
GGCCGCGTCCAGCTCCAGTCCTCCTTCAAGGAGTCCGGCTTCGACTCGCTCACCGCCGTC
GAACTCCGCAACCGGCTCACCGCGGCCACCGGAACGAAGCTTCCCGCCACCCTCGTCTTC
GACCATCCGACACCCGCGGCACTCGTCGACCACCTGGAACAGGAACTGCCGAAGGCAGCA
CAGGAGATCCCGGCGGACCTCCCGGCCGTTCTCGACGCACTCGACCGGATCCGGGACGGA
CTCGCCACCGCCGCCACCGACGACAGCAGCCGCGACCACATCGCGGAGCGTCTCCAGGCG
TTGCTCGGCACGCTCACCTCGGCTGCGGGCGTCAGCCGCCCGACCGGCAGCCCGGGCGAG
CACGACCGGCAGGGCCCCGATGAGCTGTCGCTCGGCCAACGACTCGCCGCCAGCAGCGAC
GACGAACTCTTCGACCTCTTCGACAGCGACTTCCGATCGACGTAG
[3] KS35..407
[3] AT566..913
[3] malonyl-CoA762..766
[3] DH961..1141
[3] KR1488..1668
[3] ACP1803..1873
[3] KS103..1221
[3] AT1696..2739
[3] malonyl-CoA2284..2298
[3] DH2881..3423
[3] KR4462..5004
[3] ACP5407..5619

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001227 Acyl transferase domain (Domain)
[552-690] 1.29999999999998e-74 G3DSA:3.40.366.10 [762-902] 1.29999999999998e-74 G3DSA:3.40.366.10
G3DSA:3.40.366.10   Ac_transferase_reg
IPR006162 Phosphopantetheine attachment site (PTM)
[1831-1846] PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
[1802-1877] 1.3e-23 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
[1806-1872] 4.5e-14 PF00550
PF00550   PP-binding
[1795-1890] 8.49999291746323e-24 SSF47336
SSF47336   ACP_like
[1803-1873] PS50075
PS50075   ACP_DOMAIN
IPR013968 Polyketide synthase, KR (Domain)
[1488-1667] 4.2e-61 PF08659
PF08659   KR
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[35-282] 4.30000000000003e-90 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[290-407] 6.49999999999992e-49 PF02801
PF02801   Ketoacyl-synt_C
IPR014043 Acyl transferase (Domain)
[566-913] 8.90000000000015e-65 PF00698
PF00698   Acyl_transf_1
IPR015083 Polyketide synthase, docking (Domain)
[3-27] 2.6e-12 PF08990
PF08990   Docking
[5-35] 1.6999992228405e-07 SSF101173
SSF101173   Polyketide_synth_docking
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain)
[562-856] 7.40000780398351e-69 SSF52151
SSF52151   Acyl_Trfase/lysoPlipase
IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain)
[692-761] 4.30000170645867e-16 SSF55048
SSF55048   Malonyl_transacylase_ACP-bd
IPR016038 Thiolase-like, subgroup (Domain)
[34-293] 3.60000000000002e-89 G3DSA:3.40.47.10 [294-460] 1.6e-64 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[27-406] 8.89993011983204e-103 SSF53901
SSF53901   Thiolase-like
IPR016040 NAD(P)-binding domain (Domain)
[1487-1690] 1.5e-34 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
[195-211] PS00606
PS00606   B_KETOACYL_SYNTHASE
IPR020801 Polyketide synthase, acyl transferase domain (Domain)
[566-894] 1.79999754022378e-121 SM00827
SM00827   PKS_AT
IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain)
[1804-1876] 7.79998304125571e-35 SM00823
SM00823   PKS_PP
IPR020807 Polyketide synthase, dehydratase domain (Domain)
[961-1141] 1.90000694315261e-65 SM00826
SM00826   PKS_DH
IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain)
[37-461] SM00825
SM00825   PKS_KS
IPR020842 Polyketide synthase/Fatty acid synthase, KR (Domain)
[1488-1668] 6.99997659850661e-57 SM00822
SM00822   PKS_KR
SignalP No significant hit
TMHMM No significant hit
Chalc_00210 Chalc_00210   Chalc_00230 Chalc_00230
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