Chalc_00240 : CDS information

Location

Organism	Streptomyces bikiniensis
Strain	NRRL 2737
Entry name	Chalcomycin
Contig	AY509120
Start / Stop / Direction	54,439 / 59,277 / + [in whole cluster] 54,439 / 59,277 / + [in contig]
Location	54439..59277 [in whole cluster] 54439..59277 [in contig]
Type	CDS
Length	4,839 bp (1,612 aa)

Annotation

Category	1.1 PKS
Product	polyketide synthase
Product (GenBank)	polyketide synthase subunit
Gene
Gene (GenBank)	chmGIV
EC number
Keyword
Note
Note (GenBank)	module 6 KS/AT/KR/ACP
Reference	ACC Q5SFB1 PmId [15561847] Chalcomycin biosynthesis gene cluster from Streptomyces bikiniensis: novel features of an unusual ketolide produced through expression of the chm polyketide synthase in Streptomyces fradiae. (Antimicrob Agents Chemother. , 2004) comment Chalcomycin biosynthesis gene clusterに関する文献。 chmGIV(1,612a.a.): PKS, module 6 tyl PKS genesを削除したS.fradiae K159-1でchm PKS genesを発現すると、5-O-mycaminosylchalcolactone産生。よって、これらgenesはchalcomycin PKSであり、同定したclusterはchalcomycin生合成をする。

PKS/NRPS Module

methylmalonyl-CoA

KS	36..411

AT	564..881

KR	1162..1341

ACP	1454..1524

Sequence

>polyketide synthase [polyketide synthase subunit]
MATEHEQKLRDYLKRATTELHKATERLKEVEQRAHEPVAIVGMGCRFPGGASSPEELWDL
VAAETDAVSPFPVDRGWDVTGLYDPDPDAAGRAYVREGGFLHDAGEFDAGFFGISPREAL
AMDPQQRLLLETSWEALERAGIDPHTLRGTRTGVYMGAWNGGYAEGIPQPTAELEAQLLT
GGVVSFTSGRVSYLLGLEGPAVTVDTACSSSLVALHLAVRALRSGECDLALAGGATVMST
PDVFVRFSRQRGVAADGRCKAFSASADGFGPAEGVGVLAVERLSDAVRHGRRVLAVVRGS
AVNQDGASNGLTAPSGRAQALLIRRALADARLGVADVDVVEGHGTGTRLGDPIEAQALLA
TYGQRDAGRPLRLGSLKSNVGHTQAAAGVAGVIKMVMAMRHGVLPKTLHVDEPTAEVDWS
AGAVSLLREQEAWPEVGRLRRAAVSSFGVSGTNAHVVVEEAPVPEDGEAVGGGVPLAVVP
VVVSGRSAGAVAELAGRVSEVAASGRLVDVGLSSVVSRSVFEHRSVVLAGDSAELNAGLD
AVAGGVPSPGVVSGVASGEGGRSVFVFPGQGTQWAGMALGLWAESSVFAESMARCEAAFV
GLVDWRLSQVLSDGSALERVEVVQPASFAVMVSLAELWRSLGVVPDAVVGHSQGEIAAAV
VAGGLSLEDGARVVVLRARLIGRELAGRGGMASVALPVAVVEERLAGWAGRLGVAVVNGP
SATVVAGDVDAVAEFVAACEVEGVRARVLPVDYASHSAHVEDLKAELEQILAGIGPVTGG
IPFYSTSEAAQIDTAGLDAGYWFGNLRRPVRFQETVERLLADGFRVFVECGAHPVLTGAV
QETAESTGRQVCAVGSLRRDEGGLRRFLTSAAEAFVQGVEVSWPALFEGTGARTVDLPTY
PFQRRRYWLESRPPAAPIETAAASGIESWRYRVAWKSLSLSESSRLDGRWLLVVPETLDA
DGTRIAHDIQHALTTHGATVSRLTVDVTTTDRADLSARLTTTAAEDQGPLRGVLSLLSTD
ERQHPDHPGVDRATAGTMLLAQACGDLVVARGVEPRLWVVTRGAVAVSPAERPSSAGAQV
WGLGRCAALELPTRWGGMVDLPPAARDAGRHVRRLVRLLSETCAEDQVALRASGAYGRRL
LPASSPSVSVPRTAKSGYQPRGTVLVTGGTGALGGHLARWLARNGAEHIVLAGRRGEGAP
GAAELSAELKELGAEVTVAACDVADRNALRDMLESLPADRPLSGVFHAAGVPHSAPLAET
DVAGLAAVLPGKVVGARHLHELTREKELDAFVLYASGAGVWGSGGQSAYGAANAALDALA
EQRRAEGLPATSVSWGLWDGGGMAGERGEEFLTALGLRAMEPESAVAALEEALDRGDTCV
SVVDVDWSRFAESFTAFRPSPLIGELPGVRAVPDGSAGGPSDDLADAARHGGAADRGVPA
GLARATGDDRQDILLDLVRRHAAAVLGHPGPQHIEPDAGFRTLGFSSVTAVELANKLGAA
VGTKIPATFAFDHPNARAAASRLDVLLAASSDETAQEAEIRQALRTVPLARLRAAGLLDG
LLELAGLEAEPGLPGDVPDRGAATPDEESALAEVDGLDAEALVDLVLNQSDS

>polyketide synthase [polyketide synthase subunit]
ATGGCGACCGAACACGAGCAGAAGCTCCGCGACTACCTCAAGCGGGCCACCACCGAACTC
CACAAGGCCACGGAACGGCTGAAGGAGGTCGAACAACGCGCTCACGAGCCGGTTGCGATC
GTGGGGATGGGATGCCGGTTCCCGGGCGGGGCGTCCTCGCCTGAGGAGTTGTGGGACCTG
GTGGCGGCGGAGACGGACGCGGTCTCCCCCTTCCCGGTGGACCGGGGGTGGGACGTGACG
GGGCTGTACGACCCGGATCCGGACGCGGCAGGGCGTGCCTACGTCCGCGAGGGCGGGTTC
CTCCACGACGCGGGGGAGTTCGACGCGGGGTTCTTCGGAATCTCTCCGCGTGAGGCGTTG
GCGATGGATCCGCAGCAGCGGTTGCTGCTGGAGACGTCGTGGGAGGCGTTGGAGCGGGCG
GGCATCGATCCGCACACGCTGCGCGGCACGCGGACCGGGGTCTACATGGGTGCCTGGAAC
GGCGGATACGCCGAGGGGATTCCCCAACCCACGGCGGAACTGGAGGCCCAGCTCCTCACC
GGCGGCGTGGTGAGCTTCACCTCGGGCCGTGTGTCCTACCTCCTGGGTCTGGAGGGGCCG
GCGGTGACGGTGGACACGGCGTGTTCGTCGTCGCTGGTCGCGCTGCACCTGGCGGTGCGG
GCGCTGCGCAGTGGTGAGTGCGACCTCGCCCTCGCCGGCGGCGCGACGGTGATGTCGACG
CCCGACGTGTTCGTGCGCTTCTCCCGGCAGCGAGGAGTGGCCGCGGACGGTCGCTGCAAG
GCGTTCTCCGCGTCGGCCGACGGATTCGGACCGGCTGAGGGTGTGGGCGTGCTGGCGGTG
GAGCGGTTGTCGGATGCGGTGCGTCATGGGCGTCGGGTGCTGGCGGTCGTGCGTGGTTCC
GCGGTCAACCAGGACGGTGCGAGCAACGGACTGACGGCGCCGAGCGGACGAGCTCAGGCC
CTTCTGATTCGTCGAGCGTTGGCGGATGCGCGGTTGGGTGTGGCTGATGTGGATGTGGTG
GAGGGGCATGGGACGGGGACGCGTCTGGGTGATCCGATCGAGGCGCAGGCGTTGTTGGCG
ACGTATGGGCAGCGGGATGCGGGTCGGCCGTTGCGGCTTGGTTCGTTGAAGTCGAATGTG
GGGCATACGCAGGCGGCTGCCGGTGTGGCGGGCGTGATCAAGATGGTCATGGCGATGCGG
CACGGTGTCCTGCCGAAGACGCTGCACGTGGATGAGCCGACGGCGGAGGTGGACTGGTCG
GCCGGCGCGGTGTCTTTGCTGAGGGAGCAGGAGGCGTGGCCTGAGGTGGGGCGTCTGCGT
AGGGCTGCGGTGTCTTCGTTCGGTGTGAGTGGGACGAACGCGCATGTGGTGGTGGAGGAG
GCGCCGGTTCCGGAGGACGGGGAGGCGGTCGGGGGCGGTGTGCCTTTGGCTGTGGTGCCG
GTGGTGGTGTCGGGTCGTTCTGCGGGTGCGGTGGCGGAGCTGGCGGGCCGGGTCAGCGAG
GTGGCTGCGTCTGGTCGGTTGGTGGATGTGGGGTTGTCGTCGGTGGTGTCGCGGTCGGTG
TTCGAGCACCGGTCCGTGGTACTGGCGGGGGACTCTGCCGAGCTGAATGCCGGTTTGGAT
GCTGTGGCCGGTGGTGTGCCGTCGCCTGGTGTGGTGTCGGGTGTGGCGTCGGGTGAGGGT
GGCCGGTCGGTGTTCGTGTTCCCTGGTCAGGGGACGCAGTGGGCGGGGATGGCGCTCGGG
TTGTGGGCGGAGTCGTCGGTGTTCGCGGAGTCGATGGCGCGGTGTGAGGCGGCGTTCGTC
GGCTTGGTGGACTGGCGCTTGTCGCAGGTTTTGAGCGATGGGTCGGCGCTGGAGCGGGTG
GAGGTGGTGCAGCCGGCGTCGTTCGCGGTGATGGTGTCGCTTGCTGAGCTGTGGCGGTCG
TTGGGTGTGGTGCCGGATGCGGTGGTGGGGCATTCGCAGGGGGAGATCGCTGCTGCGGTG
GTGGCGGGTGGTTTGTCGCTGGAGGACGGGGCGCGTGTGGTGGTGTTGCGTGCGCGGTTG
ATCGGTCGTGAGCTGGCCGGGCGCGGTGGGATGGCGTCGGTGGCGCTGCCGGTCGCGGTG
GTGGAGGAGCGTCTGGCGGGGTGGGCGGGGCGTCTGGGTGTGGCGGTGGTCAACGGACCG
TCCGCCACGGTCGTCGCGGGTGATGTGGATGCGGTGGCGGAGTTCGTGGCCGCGTGCGAG
GTGGAGGGGGTTCGGGCGCGTGTTCTGCCGGTGGACTACGCCTCGCACTCGGCGCACGTG
GAGGACCTGAAAGCCGAGCTTGAACAGATTCTGGCCGGCATCGGCCCGGTGACCGGTGGG
ATCCCGTTCTATTCGACGTCCGAAGCCGCGCAGATCGACACGGCTGGTCTGGACGCGGGG
TACTGGTTCGGGAATCTGCGTCGGCCGGTGCGGTTCCAGGAGACGGTCGAGCGGTTGTTG
GCGGATGGTTTCCGGGTGTTCGTGGAGTGTGGCGCGCATCCGGTGCTGACGGGGGCGGTG
CAGGAGACCGCGGAATCCACCGGTCGCCAGGTGTGTGCGGTCGGATCCCTGCGTCGTGAC
GAGGGAGGTCTGCGCCGCTTCCTCACCTCGGCCGCGGAGGCATTCGTCCAAGGCGTGGAG
GTGTCCTGGCCGGCACTGTTCGAAGGCACCGGCGCCCGCACGGTCGACCTGCCCACCTAC
CCCTTCCAACGTCGGCGCTACTGGCTGGAGTCGCGCCCTCCCGCGGCGCCGATCGAGACT
GCCGCAGCCTCTGGCATCGAGAGCTGGCGCTACCGCGTGGCGTGGAAGAGCCTGTCGCTG
TCGGAGTCGTCGCGTCTTGACGGCCGGTGGCTGCTCGTCGTGCCCGAAACCCTGGACGCC
GACGGCACGCGGATCGCCCACGACATCCAGCACGCCCTCACCACCCACGGCGCCACGGTC
TCCCGTCTGACGGTCGACGTGACGACGACCGACCGCGCCGACCTGTCGGCGCGGCTCACC
ACCACCGCGGCCGAAGACCAGGGGCCTCTCCGGGGCGTCCTCTCCCTCCTGTCCACCGAT
GAACGGCAGCACCCGGATCATCCCGGTGTCGACCGTGCCACGGCGGGCACGATGCTGCTC
GCCCAGGCGTGCGGGGATCTGGTCGTGGCCCGGGGCGTGGAGCCGAGGCTGTGGGTCGTG
ACCCGCGGGGCGGTCGCGGTGTCCCCCGCCGAGCGTCCGTCGTCAGCCGGCGCCCAGGTC
TGGGGCCTGGGGCGCTGCGCGGCGCTCGAACTTCCCACTCGGTGGGGTGGGATGGTCGAC
CTTCCCCCGGCGGCCCGGGATGCTGGAAGGCACGTACGGCGGCTCGTGCGTCTGCTGTCG
GAGACCTGTGCGGAGGACCAGGTGGCGCTGCGTGCGTCGGGTGCGTACGGCCGCAGGCTG
CTGCCCGCGTCCAGCCCCTCCGTATCCGTCCCCCGGACCGCGAAGAGCGGCTACCAGCCG
CGCGGCACGGTGCTGGTGACCGGCGGAACCGGTGCCCTCGGTGGCCACTTGGCACGGTGG
CTGGCCCGCAACGGCGCCGAGCACATCGTTCTGGCCGGGCGTCGGGGCGAGGGTGCTCCA
GGAGCCGCGGAACTGTCGGCGGAGCTCAAGGAGCTGGGTGCGGAGGTCACCGTCGCGGCC
TGCGACGTGGCGGACCGGAACGCGTTGCGTGACATGCTGGAATCCCTGCCGGCCGACCGG
CCGCTGTCGGGGGTGTTCCACGCTGCCGGTGTCCCGCACTCGGCGCCGCTGGCCGAGACG
GATGTGGCGGGGCTCGCCGCCGTGCTCCCGGGGAAGGTCGTCGGGGCACGGCACCTGCAC
GAACTCACCAGGGAGAAGGAACTGGACGCGTTCGTGCTGTACGCGTCGGGCGCCGGGGTG
TGGGGGAGCGGCGGGCAGAGCGCGTACGGAGCCGCCAACGCCGCACTGGACGCGCTGGCC
GAACAGCGCCGGGCTGAGGGACTGCCCGCCACTTCGGTCTCCTGGGGCCTGTGGGACGGC
GGAGGCATGGCCGGCGAGCGAGGCGAGGAGTTCCTCACCGCCCTCGGCCTGCGGGCCATG
GAGCCCGAGTCGGCTGTCGCCGCCCTGGAGGAGGCCCTGGATCGTGGGGACACCTGCGTG
AGCGTGGTCGACGTCGACTGGTCCCGGTTCGCCGAGTCGTTCACCGCCTTCCGGCCCAGC
CCGCTGATCGGGGAGCTCCCCGGGGTACGTGCCGTGCCCGACGGATCGGCGGGCGGACCG
TCGGACGACCTCGCGGACGCTGCGCGGCACGGCGGGGCAGCCGACCGGGGTGTGCCTGCA
GGGCTCGCCCGGGCGACGGGCGACGACCGGCAGGACATCCTGCTCGATCTCGTACGCCGC
CATGCCGCCGCCGTCCTCGGTCACCCGGGACCGCAGCACATCGAGCCCGACGCCGGTTTC
CGGACCCTGGGGTTCAGTTCGGTCACCGCGGTGGAACTGGCCAACAAGCTCGGTGCGGCC
GTGGGAACGAAGATCCCCGCCACCTTCGCGTTCGACCACCCCAACGCCCGTGCCGCGGCG
TCCCGCCTCGACGTCCTGTTGGCGGCGTCGAGCGATGAGACCGCGCAGGAGGCGGAGATC
CGGCAGGCACTGCGGACTGTGCCGCTGGCCCGGCTGCGGGCTGCGGGGCTCCTCGACGGC
CTGCTCGAACTCGCCGGGCTGGAAGCGGAGCCCGGCCTGCCGGGCGACGTACCGGATCGC
GGTGCGGCCACGCCGGACGAGGAGTCCGCCCTGGCGGAAGTCGACGGCCTGGACGCCGAA
GCACTGGTCGACCTCGTCCTCAACCAGTCCGACTCCTGA

[6] KS	36..411

[6] AT	564..881

[6] methylmalonyl-CoA	753..757

[6] KR	1162..1341

[6] ACP	1454..1524

[6] KS	106..1233

[6] AT	1690..2643

[6] methylmalonyl-CoA	2257..2271

[6] KR	3484..4023

[6] ACP	4360..4572

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001227 Acyl transferase domain (Domain) G3DSA:3.40.366.10 Ac_transferase_reg IPR006162 Phosphopantetheine attachment site (PTM) PS00012 PHOSPHOPANTETHEINE IPR009081 Acyl carrier protein-like (Domain) SSF47336 ACP_like PF00550 PP-binding G3DSA:1.10.1200.10 ACP_like PS50075 ACP_DOMAIN IPR013968 Polyketide synthase, KR (Domain) PF08659 KR IPR014030 Beta-ketoacyl synthase, N-terminal (Domain) PF00109 ketoacyl-synt IPR014031 Beta-ketoacyl synthase, C-terminal (Domain) PF02801 Ketoacyl-synt_C IPR014043 Acyl transferase (Domain) PF00698 Acyl_transf_1 IPR015083 Polyketide synthase, docking (Domain) PF08990 Docking SSF101173 Polyketide_synth_docking IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain) SSF52151 Acyl_Trfase/lysoPlipase IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain) SSF55048 Malonyl_transacylase_ACP-bd IPR016038 Thiolase-like, subgroup (Domain) G3DSA:3.40.47.10 Thiolase-like_subgr IPR016039 Thiolase-like (Domain) SSF53901 Thiolase-like IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd IPR018201 Beta-ketoacyl synthase, active site (Active_site) PS00606 B_KETOACYL_SYNTHASE IPR020801 Polyketide synthase, acyl transferase domain (Domain) SM00827 PKS_AT IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain) SM00823 PKS_PP IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain) SM00825 PKS_KS IPR020842 Polyketide synthase/Fatty acid synthase, KR (Domain) SM00822 PKS_KR
SignalP	No significant hit
TMHMM	No significant hit

Chalc_00230 Chalc_00250