Chalc_00240 Chalc_00240   Chalc_00260 Chalc_00260

Chalc_00250 : CDS information

close this sectionLocation

Organism
StrainNRRL 2737
Entry nameChalcomycin
Contig
Start / Stop / Direction59,387 / 63,439 / + [in whole cluster]
59,387 / 63,439 / + [in contig]
Location59387..63439 [in whole cluster]
59387..63439 [in contig]
TypeCDS
Length4,053 bp (1,350 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase
Product (GenBank)polyketide synthase subunit
Gene
Gene (GenBank)chmGV
EC number
Keyword
Note
Note (GenBank)
  • module 7 and terminal thioesterase
Reference
ACC
PmId
[15561847] Chalcomycin biosynthesis gene cluster from Streptomyces bikiniensis: novel features of an unusual ketolide produced through expression of the chm polyketide synthase in Streptomyces fradiae. (Antimicrob Agents Chemother. , 2004)
comment
Chalcomycin biosynthesis gene clusterに関する文献。

chmGV(1,350a.a.): PKS, module 7 and thioesterase

tyl PKS genesを削除したS.fradiae K159-1でchm PKS genesを発現すると、5-O-mycaminosylchalcolactone産生。よって、これらgenesはchalcomycin PKSであり、同定したclusterはchalcomycin生合成をする。

close this sectionPKS/NRPS Module

7 malonyl-CoA
KS35..414
AT579..912
ACP972..1046
TE1137..1347

close this sectionSequence

selected fasta
>polyketide synthase [polyketide synthase subunit]
MALSQEKVLEALRTSVKDAERLRKRNRELLAARHEPIAVVGMACRYPGGVRSPEDLWELV
VSGTDAVGPFPEDRGWDVERIYDQDPSVPGTTYCREGGFLYDAGDFDAAFFGIGPREATV
MDPQQRQLLEASWEALEQAGLDPRALRGSQGGVFVGAANQGYVPGDAAASGRLPEGSDGY
LLTGNADAVLSGRISYFLGLEGPSMTVETACSSSLVALHLAVQALRREECEFALAGGVAV
LANPAAYVEFARQRGLAPDGRCKAFDDAADGTGWAEGVGVLVVERLSDAVRKGHRVLAVV
RGTAVNQDGASSGLSVPNGPSQQRVIRRALADARLEAGQIDAVEAHGTGTRLGDPIEAQA
LLDTYGEERSPERPLWVGSLKSNFGHAQAAAGVGGVIKTVMALRHGLLPRTLHVTSPTRH
VDWGDGQVRLLTEPVDWPRTGAPRRAAVSAFGVSGTNGHIILEEAPPPTRPEAVRQAGER
RPVLVPWTLSGRTRPALCRQAARLAAHLEQHPDLDPLDVGFSLATTRTHFEHRAVLLADA
ATEGGSRADALGALRAIAEDRDPGGAVRDTARGEGRIAFLFCGQGSQRPGMAEQLYAQYP
AFARELDTIATHLDAHLDRPLATVMFAPAGTAEAALLDGTQYAQAALFAVEVALFRLFEG
WGLRPDVLLGHSVGELAAAHVAGVFGPADACSLVAARGRLMQELPAGGAMLSVRAAEHEV
RELIAGQEDRIAVAAVNGPRSVVVSGDEDAVSALAEELTEYGVRTKRLNVSHAFHSPRLD
SMLETFRRVAETVEYREPTLDVISGLTGRPADAGELATADYWVRQARETVRFHDGVRAAH
ARGVSTFVELGPDGVLCGLALETLAEETDGEAAAETPGRARAALVPVMRRERPEGSTLLT
ALATAHARGAEVDWSRFYADTGARHTTLPTYAFQRQRFWLETAAPAAPAAGQGAGPADPQ
DSTGPAARPTLTEQDLLLLVRTEAAAALGHAELEDVPADSLFGDIGFDSLAAIELGAALT
GATGLEVPSSLVLDHPTPRELAAHLAAARTAADSDDTSPEGPDTAGESSLSAMYRRAVRL
GRAEPFIGTLAELAAFRPVFPADHTLADGETVGQAAAAWQPAPVRLATTDGEGPELICCA
GTAVASGPEEFTALAAALGDRLTVSALRQPGFRANELLPGSLDGLLDAQADAVLRHTGDR
PYALLGHSAGGALAHALACRLEELGAGPAALVLADVYLPSSPGAMGVWRNEMLDWVMRRS
VVSIDDARLTAMGAYNQMLLEWTPRPTKAPVLFLRATEPVRPWSGEPESWRAHWDGGDHT
AVDVPGTHLTLMTEHARHLAATLHTWLGTL
selected fasta
>polyketide synthase [polyketide synthase subunit]
ATGGCTCTGTCCCAAGAGAAGGTACTGGAGGCACTGCGCACCTCCGTCAAGGACGCCGAA
CGGCTGCGCAAGCGCAACCGCGAACTCCTCGCGGCCCGCCACGAGCCCATCGCCGTCGTC
GGCATGGCCTGCCGCTATCCCGGCGGGGTCCGTTCGCCCGAGGACCTCTGGGAACTCGTC
GTGTCGGGCACGGACGCGGTGGGTCCCTTTCCCGAGGACCGTGGCTGGGACGTGGAGCGG
ATCTACGACCAGGACCCGTCCGTCCCGGGCACCACGTACTGCCGCGAGGGCGGATTCCTT
TACGATGCGGGGGACTTCGACGCGGCTTTCTTCGGGATAGGGCCGCGCGAGGCCACCGTG
ATGGACCCCCAGCAGCGCCAGCTGCTGGAGGCGTCCTGGGAAGCCCTGGAGCAGGCCGGG
CTGGACCCCCGGGCGCTCCGCGGCAGCCAGGGGGGTGTGTTCGTCGGCGCGGCGAACCAG
GGCTACGTACCGGGTGACGCGGCGGCGTCGGGACGTCTGCCGGAGGGTTCCGACGGCTAT
CTGCTCACCGGCAACGCCGACGCCGTCCTGTCGGGCCGGATCAGCTACTTCCTGGGCCTG
GAAGGCCCGTCCATGACCGTCGAGACGGCCTGCTCCTCCTCCCTGGTGGCACTGCACCTG
GCGGTGCAGGCGCTGCGCCGTGAGGAGTGCGAGTTCGCCCTGGCCGGAGGGGTCGCCGTG
CTCGCCAACCCGGCCGCCTACGTGGAGTTCGCCCGGCAGCGGGGACTCGCCCCGGACGGG
CGCTGCAAGGCGTTCGACGACGCGGCGGACGGTACGGGCTGGGCCGAGGGCGTCGGCGTC
CTGGTGGTGGAGCGGCTGTCGGACGCGGTACGCAAGGGGCACCGGGTCCTCGCCGTCGTG
CGGGGCACGGCGGTGAACCAGGACGGTGCCAGCAGCGGTCTGTCCGTGCCCAACGGGCCC
TCCCAGCAGCGGGTCATCCGCCGAGCGCTGGCCGACGCCCGGCTGGAGGCCGGCCAGATC
GACGCGGTGGAGGCCCACGGCACCGGCACTCGGCTGGGGGACCCCATCGAGGCGCAAGCC
CTGCTGGACACGTACGGAGAGGAGCGGAGCCCCGAACGCCCTCTGTGGGTCGGGTCGTTG
AAGTCGAACTTCGGTCACGCACAGGCGGCAGCCGGAGTCGGCGGCGTCATCAAGACGGTG
ATGGCGCTCCGGCACGGCCTGCTTCCCCGCACGCTCCATGTGACCAGCCCGACGCGGCAC
GTCGACTGGGGCGACGGACAGGTGCGGCTGCTGACCGAGCCGGTCGACTGGCCGCGGACC
GGCGCCCCCCGGCGGGCCGCGGTCTCGGCGTTCGGCGTGAGCGGCACCAACGGGCACATC
ATCCTCGAGGAGGCGCCGCCGCCCACCCGGCCCGAAGCGGTCCGGCAGGCCGGGGAGCGG
CGGCCGGTCCTGGTCCCGTGGACGCTGTCCGGCCGTACGAGGCCGGCGCTGTGCCGGCAG
GCCGCGCGCCTGGCGGCGCACCTCGAACAGCACCCGGACCTCGACCCGCTGGACGTCGGG
TTCTCGCTCGCCACGACGCGCACCCACTTCGAGCACCGGGCCGTGCTGCTCGCGGACGCC
GCCACCGAGGGCGGCTCCCGTGCCGACGCGCTCGGGGCGTTGCGGGCGATCGCGGAGGAC
CGCGACCCGGGCGGGGCGGTACGGGACACCGCGCGGGGCGAAGGGCGTATCGCCTTCCTG
TTCTGCGGGCAGGGCAGCCAGCGGCCCGGCATGGCGGAGCAGCTGTACGCGCAGTACCCG
GCGTTCGCGCGGGAACTGGACACGATCGCGACGCATCTGGACGCCCATCTGGACCGTCCG
TTGGCGACGGTGATGTTCGCGCCGGCCGGTACGGCGGAGGCCGCGCTGCTCGACGGCACG
CAGTACGCCCAGGCGGCCCTGTTCGCCGTAGAGGTCGCGTTGTTCCGGCTCTTCGAGGGC
TGGGGGCTGCGCCCCGACGTACTGCTGGGCCATTCCGTGGGCGAGCTGGCCGCCGCCCAC
GTGGCCGGGGTGTTCGGGCCGGCGGACGCCTGCTCGCTGGTCGCCGCACGCGGCCGGCTC
ATGCAGGAGCTGCCGGCCGGCGGCGCGATGCTCTCGGTCCGTGCCGCCGAGCACGAGGTG
CGGGAGCTGATCGCCGGGCAGGAGGACCGCATCGCGGTGGCGGCCGTCAACGGGCCCCGC
TCCGTGGTCGTATCCGGGGACGAGGACGCGGTCTCGGCGCTCGCCGAGGAGCTGACCGAA
TACGGCGTGCGCACCAAGCGCCTCAACGTCAGCCACGCCTTCCACTCCCCACGTCTGGAC
TCCATGCTGGAGACGTTCCGCCGGGTCGCGGAGACGGTGGAGTACCGCGAGCCGACGCTC
GACGTGATCAGCGGCCTGACCGGCCGCCCGGCCGACGCCGGGGAACTCGCCACCGCCGAC
TACTGGGTCCGGCAGGCGAGGGAGACCGTCCGGTTCCACGACGGGGTGCGCGCCGCGCAC
GCGCGCGGCGTCAGCACCTTCGTGGAGCTGGGGCCGGACGGCGTGCTGTGCGGCCTGGCC
CTGGAGACCCTGGCGGAGGAGACCGACGGGGAAGCGGCCGCCGAGACGCCCGGCCGGGCG
CGGGCGGCGCTGGTGCCCGTGATGCGTCGGGAGCGGCCGGAGGGCAGTACCCTCCTGACG
GCGCTCGCCACGGCCCACGCGCGCGGGGCGGAGGTGGACTGGTCCCGGTTCTACGCCGAC
ACCGGCGCCCGCCACACCACACTGCCCACCTATGCCTTCCAGCGCCAGCGGTTCTGGCTG
GAGACGGCGGCCCCCGCCGCGCCCGCGGCGGGCCAGGGGGCCGGACCGGCCGACCCGCAG
GACAGCACCGGTCCGGCCGCCCGGCCCACGCTGACGGAACAGGACCTCCTCCTGCTCGTG
CGGACGGAAGCGGCGGCCGCACTCGGCCACGCCGAACTGGAGGACGTACCGGCCGACAGC
CTCTTCGGCGACATCGGCTTCGACTCGCTCGCGGCCATCGAACTGGGCGCCGCCCTGACC
GGCGCCACCGGGCTGGAAGTGCCGTCGTCCCTCGTCCTCGACCACCCCACGCCCAGGGAG
CTGGCCGCGCACCTGGCAGCCGCCCGGACGGCCGCCGACAGCGACGACACGTCCCCCGAA
GGCCCGGACACGGCCGGTGAGAGCAGCCTGTCGGCGATGTACCGGCGGGCCGTGCGGCTC
GGCCGGGCCGAGCCGTTCATCGGCACACTCGCCGAACTCGCCGCCTTCCGGCCCGTCTTC
CCCGCCGATCACACCCTCGCGGACGGCGAGACCGTCGGACAGGCGGCCGCCGCCTGGCAG
CCGGCTCCGGTGCGCCTGGCCACCACGGACGGTGAGGGACCGGAGCTGATCTGCTGCGCG
GGTACGGCGGTGGCGTCGGGACCGGAGGAGTTCACCGCGCTGGCCGCGGCCCTGGGCGAC
CGGCTGACCGTGTCGGCACTGCGCCAGCCCGGCTTCCGCGCGAACGAGTTGCTGCCCGGC
TCCCTGGACGGGCTGCTCGACGCGCAGGCGGACGCGGTGCTGCGGCACACGGGTGACAGG
CCCTACGCCCTCCTCGGCCACTCGGCGGGCGGGGCGCTGGCGCACGCGCTGGCCTGCCGA
CTGGAGGAGCTCGGCGCGGGTCCCGCGGCGCTGGTCCTGGCCGACGTCTATCTGCCCAGC
TCGCCGGGGGCGATGGGGGTGTGGCGCAACGAGATGCTCGACTGGGTCATGCGGCGTTCC
GTGGTGTCCATCGACGATGCCCGGTTGACGGCCATGGGCGCCTACAACCAGATGCTCCTG
GAGTGGACACCGCGGCCCACGAAGGCGCCGGTCCTGTTCCTGCGCGCGACGGAGCCGGTG
AGGCCGTGGTCCGGAGAACCGGAGAGCTGGCGGGCGCACTGGGACGGCGGCGACCACACC
GCCGTCGACGTGCCCGGCACCCACCTGACGCTGATGACCGAGCACGCCCGCCACCTCGCG
GCGACCCTCCACACCTGGCTCGGCACCCTGTGA
[7] KS35..414
[7] AT579..912
[7] malonyl-CoA772..776
[7] ACP972..1046
[7] TE1137..1347
[7] KS103..1242
[7] AT1735..2736
[7] malonyl-CoA2314..2328
[7] ACP2914..3138
[7] TE3409..4041

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001031 Thioesterase (Domain)
[1137-1347] 4.2e-37 PF00975
PF00975   Thioesterase
IPR001227 Acyl transferase domain (Domain)
[571-704] 7.69999999999997e-74 G3DSA:3.40.366.10 [772-901] 7.69999999999997e-74 G3DSA:3.40.366.10
G3DSA:3.40.366.10   Ac_transferase_reg
IPR006162 Phosphopantetheine attachment site (PTM)
[1004-1019] PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
[968-1063] 3.00000067992871e-18 SSF47336
SSF47336   ACP_like
[974-1048] 1.5e-18 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
[980-1045] 9e-12 PF00550
PF00550   PP-binding
[972-1046] PS50075
PS50075   ACP_DOMAIN
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[35-289] 1.5e-95 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[297-414] 1.29999999999998e-45 PF02801
PF02801   Ketoacyl-synt_C
IPR014043 Acyl transferase (Domain)
[579-912] 6.60000000000002e-69 PF00698
PF00698   Acyl_transf_1
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain)
[576-866] 3.30001976117415e-65 SSF52151
SSF52151   Acyl_Trfase/lysoPlipase
IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain)
[706-771] 1.60000240695997e-19 SSF55048
SSF55048   Malonyl_transacylase_ACP-bd
IPR016038 Thiolase-like, subgroup (Domain)
[37-301] 2.50000000000001e-89 G3DSA:3.40.47.10 [302-468] 1.6e-61 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[27-413] 7.49999605851445e-99 SSF53901
SSF53901   Thiolase-like
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
[202-218] PS00606
PS00606   B_KETOACYL_SYNTHASE
IPR020801 Polyketide synthase, acyl transferase domain (Domain)
[580-892] 7.40000780398351e-119 SM00827
SM00827   PKS_AT
IPR020802 Polyketide synthase, thioesterase domain (Domain)
[1137-1347] 8.70005082121738e-119 SM00824
SM00824   PKS_TE
IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain)
[977-1049] 1.60000240695997e-23 SM00823
SM00823   PKS_PP
IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain)
[37-467] SM00825
SM00825   PKS_KS
SignalP No significant hit
TMHMM No significant hit
Chalc_00240 Chalc_00240   Chalc_00260 Chalc_00260
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