Chro_00200 Chro_00200   Chro_00220 Chro_00220

Chro_00210 : CDS information

close this sectionLocation

Organism
StrainATCC 13273 (=NBRC 3746)
Entry nameChromomycin
Contig
Start / Stop / Direction22,349 / 23,590 / + [in whole cluster]
22,349 / 23,590 / + [in contig]
Location22349..23590 [in whole cluster]
22349..23590 [in contig]
TypeCDS
Length1,242 bp (413 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative ligase
Product (GenBank)acyl CoA ligase
Gene
Gene (GenBank)cmmLI
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[15112992] Biosynthesis of the antitumor chromomycin A3 in Streptomyces griseus: analysis of the gene cluster and rational design of novel chromomycin analogs. (Chem Biol. , 2004)
comment
aureolic acid type antitumor drug chromomycin A3の生合成gene clusterの同定論文。

cmmLI : Acyl-CoA ligase

配列解析から機能推定。
acyl CoA ligasesに似たタンパクをコード。
AMP binding domain(Pfam00501)を含む。
Related Reference
ACC
Q8GHC3
PmId
comment
Blast 4th, id37%, 2e-47, C末100aa以上余る
Streptomyces roseochromogenes subsp. oscitans_cloL
Amide synthetase

amide synthases CloL, NovL, CouLのvitroでの基質特異性検討、vivoでの合成基質添加実験。
AA配列は相同性高いが、基質特異性はそれぞれ異なる。
CloLは多くの合成基質の受け入れが可能。
ACC
Q9L9F6
PmId
[10801869] Cloning, overexpression, and purification of novobiocic acid synthetase from Streptomyces spheroides NCIMB 11891. (J Biol Chem. , 2000)
comment
Blast 8th, id35%, 8e-42, C末100aa以上余る
Streptomyces sphaeroides_novL
NovL

Novobiocic acid synthetaseをコードするnovLをE.coliで発現、精製、活性測定。
3-dimethylallyl-4-hydroxybenzoic acid (ring A of novobiocin) と
3-amino-4,7-dihydroxy-8-methyl coumarin (ring B of novobiocin)
の間のアミド結合の形成をATP-dependent reactionで触媒した。
また、ring A and ATPからのacyl adenylateの形成がATP-PP(i) exchange assayによって実証された。
すなわちNovLは、acyl adenylateのようなring Aの活性化と、引き続くacyl基のring Bのamino基への移動の両方を触媒した。
ACC
Q9EY88
PmId
[11152072] Identification of Amycolatopsis sp. strain HR167 genes, involved in the bioconversion of ferulic acid to vanillin. (Appl Microbiol Biotechnol. , 2000)
comment
Blast 112th, id32%, 2e-30, C末100aa以上余る
Amycolatopsis sp. HR167_fcs
Feruloyl-CoA synthetase

Amycolatopsis sp. strain HR167からech (encoding enoyl-CoA hydratase/aldolase), fcs (encoding an unusual feruloyl-CoA synthetase)を含む4 kb断片をクローニングしてシークエンス。
この断片をE. coliに導入するとferulic acid → vanillinへtransformできるようになり、feruloyl-CoA synthetase活性、enoyl-CoA hydratase/aldolase活性検出された

close this sectionSequence

selected fasta
>putative ligase [acyl CoA ligase]
MEDRLCARPAARYVTELFTGLHRWSGRIALRTGKRQITFDELLAQSYRIARALERMGHSR
HEGIVCLTANPPELVALRLAAHLLGSRFTALNVNVPGDVTGWRGILASVRPAVFLVDRRG
AEVLGEPVPGTLTLGVPEAGCDLLARAAEESGEPLAPRAREEDIALLVPSHGTTGPRRGA
VHRFSGMRVDWRRPEEGALGDFPAGITTLAVSPLAGNAGEVVLMLLRQGCTALLMDDFDP
GRVLATVEAERITSVYLLSGHLRRLLAHPDVGRTDLSSLRYVPYGNAPVPAATVRRAVEV
FGPVLAQNYLSSEIRAITLLRQEDHLAAAARRPELLRSVGRALPEVEIRIRGPRDEPLPC
GACGEVWLRAPHMMSGYWRDPAGTARVMRGGWLHSGDRGRLDEEGYLYLTGRA
selected fasta
>putative ligase [acyl CoA ligase]
GTGGAGGACCGGCTGTGCGCCAGGCCAGCCGCCCGCTATGTGACCGAGCTCTTCACCGGG
CTCCACCGGTGGTCCGGCCGGATCGCGCTGCGGACGGGGAAGCGGCAGATCACGTTCGAC
GAGCTGCTGGCCCAGAGCTACCGGATCGCCCGCGCGCTGGAGCGGATGGGGCACAGCCGC
CATGAGGGCATCGTGTGCCTCACGGCGAACCCGCCCGAGCTGGTAGCCCTGCGGCTCGCC
GCCCACCTGCTGGGCAGCCGCTTCACCGCGCTCAACGTCAACGTGCCGGGGGATGTGACA
GGCTGGCGCGGGATCCTCGCCTCCGTGCGGCCCGCGGTCTTCCTGGTGGACCGGCGGGGC
GCCGAGGTGCTGGGGGAGCCGGTGCCCGGAACGCTGACGCTGGGAGTGCCGGAGGCGGGG
TGCGACCTGCTGGCCCGGGCCGCCGAGGAGTCCGGGGAGCCGTTGGCGCCGCGCGCCCGG
GAGGAGGACATCGCCCTGCTGGTCCCGTCGCACGGTACGACGGGTCCCCGGCGGGGAGCC
GTCCACCGGTTCTCCGGGATGAGGGTCGACTGGCGCCGCCCGGAGGAAGGCGCACTCGGC
GATTTCCCGGCCGGGATCACCACCCTGGCGGTCAGCCCGCTGGCGGGGAACGCGGGGGAG
GTCGTGCTCATGCTCCTCAGGCAGGGTTGCACCGCTCTGCTCATGGACGACTTCGACCCG
GGCCGGGTGCTCGCGACCGTCGAGGCCGAGCGGATCACCAGCGTGTACCTGCTCTCCGGT
CACCTGCGGCGGCTGCTCGCCCACCCGGATGTCGGCCGGACGGACCTGTCCAGCCTGCGC
TATGTGCCGTACGGTAACGCGCCTGTCCCGGCCGCCACCGTGCGGCGGGCGGTCGAGGTC
TTCGGCCCGGTCCTGGCACAGAACTACCTCAGCAGCGAGATCCGGGCCATCACCCTCCTG
CGGCAGGAGGACCACCTGGCCGCCGCGGCGCGCCGGCCGGAGCTGCTGCGGTCCGTCGGA
CGGGCGCTGCCCGAGGTCGAGATCCGGATACGCGGGCCCCGGGACGAGCCGCTTCCCTGC
GGCGCGTGTGGCGAGGTCTGGCTGCGCGCCCCGCACATGATGAGCGGCTACTGGCGCGAT
CCGGCCGGGACGGCCCGGGTCATGCGCGGAGGCTGGCTGCACTCCGGCGACCGGGGCCGG
CTGGACGAGGAGGGCTACCTCTATCTCACCGGCCGCGCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000873 AMP-dependent synthetase/ligase (Domain)
[38-413] 1.9e-52 PF00501
PF00501   AMP-binding
SignalP No significant hit
TMHMM No significant hit
Chro_00200 Chro_00200   Chro_00220 Chro_00220
Page top