Chro_00240 : CDS information

Location

Organism	Streptomyces griseus
Strain	ATCC 13273 (=NBRC 3746)
Entry name	Chromomycin
Contig	AJ578458
Start / Stop / Direction	25,986 / 25,390 / - [in whole cluster] 25,986 / 25,390 / - [in contig]
Location	complement(25390..25986) [in whole cluster] complement(25390..25986) [in contig]
Type	CDS
Length	597 bp (198 aa)

Annotation

Category	4.1 transcriptional regulator
Product	PadR family transcriptional repressor
Product (GenBank)	transcriptional regulator
Gene
Gene (GenBank)	cmmRII
EC number
Keyword
Note
Note (GenBank)
Reference	ACC Q70J74 PmId [15112992] Biosynthesis of the antitumor chromomycin A3 in Streptomyces griseus: analysis of the gene cluster and rational design of novel chromomycin analogs. (Chem Biol. , 2004) [17768249] Involvement of a chromomycin ABC transporter system in secretion of a deacetylated precursor during chromomycin biosynthesis. (Microbiology. , 2007) comment [PMID:15112992](2004) aureolic acid type antitumor drug chromomycin A3の生合成gene clusterの同定論文。 CmmRII : Transcriptional regulator 配列解析から機能推定。 ABC transporter systemを形成するgenesと一緒に転写されうる。 -- [PMID:17768249](2007) cmmRII を不活化すると、chromomycin、mithramycinに抵抗性のあるchromomycin高産生株となった。よって、おそらくchromomycin resistance/biosynthesisでのtranscriptional repressorとして働く。
Family/Domain	FD IPR005149 comment [IPR005149] Transcription regulator PadR N-terminal-like (Domain)

Sequence

>PadR family transcriptional repressor [transcriptional regulator]
MALGTLQNVGPLHGHEIRRIAEVTSVSSWGGVSVGALYRELRTMDEGGLVESVRTEQVGR
RPARTVYRITPSGEGELRDLREQAVTQVHHGTDPFGVALLFTRTSELPTLLDLLERRREA
VLTMRNDIEAESRALREAGQIGPLDAAMFRRRVMQLDADLLWQDELDRTITETVAAEQRV
GEELPADGTTGPTAEVSP

>PadR family transcriptional repressor [transcriptional regulator]
ATGGCTCTCGGGACCCTTCAGAACGTGGGACCGCTCCACGGCCACGAGATCCGCCGCATC
GCGGAAGTGACCAGCGTCAGCAGTTGGGGAGGCGTCAGCGTCGGCGCGCTCTACCGCGAG
CTGCGCACGATGGACGAAGGCGGCCTGGTCGAATCCGTCCGCACGGAACAGGTGGGCAGA
CGCCCCGCCAGGACCGTGTACCGGATCACCCCCTCGGGAGAGGGCGAGCTGCGCGACCTG
CGTGAGCAGGCGGTCACCCAGGTGCACCACGGGACGGATCCGTTCGGCGTCGCCCTCCTC
TTCACCCGCACCAGCGAACTGCCGACCCTTCTGGACCTGCTGGAGCGGCGCCGCGAAGCC
GTCCTGACCATGCGCAACGACATCGAGGCCGAGAGCCGCGCGCTGCGCGAGGCCGGCCAG
ATCGGGCCGCTCGATGCCGCGATGTTCCGGCGCAGGGTGATGCAGCTCGACGCGGACCTG
CTCTGGCAGGACGAACTCGACCGGACGATCACCGAGACGGTCGCGGCCGAACAACGCGTC
GGCGAGGAGCTTCCCGCCGACGGGACCACCGGACCCACAGCGGAGGTATCACCGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005149 Transcription regulator PadR N-terminal (Domain) PF03551 PadR IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain) G3DSA:1.10.10.10 Wing_hlx_DNA_bd
SignalP	No significant hit
TMHMM	No significant hit

Chro_00230 Chro_00250