Herb_00020 : CDS information

Location

Organism	Streptomyces hygroscopicus
Strain	AM 3672
Entry name	Herbimycin A
Contig	AY947889
Start / Stop / Direction	2,775 / 2,359 / - [in whole cluster] 2,775 / 2,359 / - [in contig]
Location	complement(2359..2775) [in whole cluster] complement(2359..2775) [in contig]
Type	CDS
Length	417 bp (138 aa)

Annotation

Category	5.4 hypothetical protein
Product	hypothetical protein
Product (GenBank)	unknown
Gene
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)	ORF2p; ORF2; similar to Streptomyces coelicolor SC00861c and Streptomyces avermitilis SAV618 putative secreted protein
Reference	ACC Q49BF6 PmId [16085885] Insights into the biosynthesis of the benzoquinone ansamycins geldanamycin and herbimycin, obtained by gene sequencing and disruption. (Appl Environ Microbiol. , 2005) comment herbimycin生合成系gene clusterのクローニングを行い、同じbenzoquinone ansamycin系のgeldanamycin のgene clusterと比較し、一部の遺伝子欠損株を作成してその生合成における関与を調べた文献。 ORF02(417bp):putative secreted protein 機能実験、詳細記載無し。

Sequence

>hypothetical protein [unknown]
MNRAEQVTRSPDEPGVMTRITARRRNSAAGRPDSSTGTAGSAEAVRSDWKFPHMAQPRPS
ARRTPGAFLSRALPPPALCGFLLLLVMVFAASYAVGAGAGPVAPGMHGSSTTDGGSGDGH
GGGGMDMGGMDTDQGSGR

>hypothetical protein [unknown]
ATGAACAGGGCTGAGCAGGTCACGCGCTCGCCGGACGAGCCCGGTGTCATGACGCGCATC
ACAGCACGGCGCCGGAACTCGGCTGCCGGTCGTCCCGACTCCTCTACCGGTACGGCCGGA
TCCGCTGAAGCCGTACGGAGTGATTGGAAGTTTCCTCATATGGCGCAGCCCCGCCCATCT
GCCCGCCGGACACCGGGTGCGTTCCTGTCCAGGGCATTGCCCCCGCCGGCGCTGTGCGGA
TTCCTGCTGCTGCTGGTGATGGTGTTCGCCGCCTCCTACGCCGTCGGCGCGGGCGCCGGG
CCCGTCGCCCCCGGCATGCACGGCAGCAGCACGACAGACGGCGGCTCGGGAGACGGCCAC
GGCGGTGGCGGCATGGACATGGGCGGCATGGACACGGACCAGGGGAGTGGACGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro	No significant hit
SignalP	No significant hit
TMHMM	Transmembrane 1

Herb_00010 Herb_00030