Griseo_00100 Griseo_00100   Griseo_00120 Griseo_00120

Griseo_00110 : CDS information

close this sectionLocation

Organism
StrainJP95
Entry nameGriseorhodin A
Contig
Start / Stop / Direction10,944 / 11,717 / + [in whole cluster]
10,944 / 11,717 / + [in contig]
Location10944..11717 [in whole cluster]
10944..11717 [in contig]
TypeCDS
Length774 bp (257 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative oxidoreductase
Product (GenBank)putative 3-oxoacyl-ACP reductase GrhO2
Gene
Gene (GenBank)grhO2
EC number
Keyword
Note
  • It is supposed that this ORF is not involved in griseorhodin A biosynthesis.
Note (GenBank)
Reference
ACC
PmId
[12323376] A gene cluster from a marine Streptomyces encoding the biosynthesis of the aromatic spiroketal polyketide griseorhodin A. (Chem Biol. , 2002)
[19175308] Cleavage of four carbon-carbon bonds during biosynthesis of the griseorhodin a spiroketal pharmacophore. (J Am Chem Soc. , 2009)
comment
[PMID:12323376](2002)
griseorhodin A生合成gene clusterのクローニング、シークエンス、異種性発現。

grhO2: 2-Oxoacyl-ACP reductase

配列解析のみ。
short chain dehydrogenase/reductase (SDR) familyのタンパクに似ている。
GrhO2のclosest homolog MtmTII は機能不明。

環化前のC19 ketoreductionを触媒する候補として、GrhO2, GrhO10, GrhTが挙げられている。

---
[PMID:19175308](2009)
grhO2: ketoreductase
grhO2 mutantでもgriseorhodin A産生可能。
Related Reference
ACC
P50205
PmId
[7582015] ( , )
comment
Blast 301st, id37%, 5e-28
Rhizobium meliloti_phbB
Acetoacetyl-CoA reductase(EC 1.1.1.36)

(R)-3-hydroxyacyl-CoA + NADP+ = 3-oxoacyl-CoA + NADPH.

E. coli に3genes; beta-ketothiolase (phaA, phbA), acetoacetyl-CoA reductase (phaB, phbB), PHB-synthase (phaC, phbC)の導入でpolyhydroxyalkanoate生成。

close this sectionSequence

selected fasta
>putative oxidoreductase [putative 3-oxoacyl-ACP reductase GrhO2]
MKTGLTDKAVLVTGASRGIGRATALAYAAEGARVAITYHSDEAGAKETAERVAAAGGTPH
VVRYDLGDEDSVRSAVASVGAEWGGVDVLVAGAVEWGEAIPRPGRKMPAFEEVPPAQWQR
VLRTSVDGVFHTVQAVLPLMRDREWGRIVVLGAGLAETGLPGASAYGAAKAALHGLVRSL
AWEVGPAGILVNEVVPGQTLTENVLAHASPAFLENKAASLPSGRMNTPEDVARAIVFLGS
AANGNIHGEALRVTGGL
selected fasta
>putative oxidoreductase [putative 3-oxoacyl-ACP reductase GrhO2]
ATGAAGACAGGACTGACGGACAAGGCGGTGCTCGTCACCGGCGCCTCGCGCGGCATCGGG
CGGGCCACGGCCCTCGCCTACGCGGCGGAGGGCGCCCGGGTCGCGATCACCTACCACTCC
GACGAGGCCGGGGCGAAGGAGACGGCCGAGCGGGTCGCCGCCGCCGGGGGCACCCCCCAC
GTCGTCCGCTACGACCTCGGGGACGAGGACTCCGTGCGCTCCGCCGTCGCCTCGGTCGGC
GCGGAGTGGGGCGGGGTCGACGTCCTCGTGGCCGGCGCGGTCGAGTGGGGGGAGGCGATT
CCGCGCCCCGGACGGAAGATGCCCGCCTTCGAGGAGGTGCCGCCCGCGCAGTGGCAACGG
GTCCTGCGCACCTCGGTGGACGGCGTCTTCCACACCGTGCAGGCGGTGCTGCCCCTCATG
CGCGACCGGGAGTGGGGCCGCATCGTGGTGCTCGGCGCCGGGCTCGCGGAGACCGGTCTG
CCGGGGGCGTCCGCGTACGGGGCCGCGAAGGCCGCCCTGCACGGGCTGGTGCGCAGCCTG
GCCTGGGAGGTCGGCCCCGCCGGGATCCTGGTCAACGAGGTCGTGCCCGGCCAGACGCTC
ACCGAGAACGTCCTCGCACACGCCTCGCCCGCCTTCCTGGAGAACAAGGCCGCCTCCCTG
CCCTCGGGCCGCATGAACACGCCCGAGGACGTCGCGCGGGCGATCGTCTTCCTCGGCTCC
GCCGCCAACGGGAACATCCACGGCGAGGCCCTGCGCGTCACCGGCGGCCTGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002198 Short-chain dehydrogenase/reductase SDR (Family)
[8-182] 9.7e-31 PF00106
PF00106   adh_short
IPR002347 Glucose/ribitol dehydrogenase (Family)
[9-26] 2.800005042611e-30 PR00081 [84-95] 2.800005042611e-30 PR00081 [140-156] 2.800005042611e-30 PR00081 [166-185] 2.800005042611e-30 PR00081 [187-204] 2.800005042611e-30 PR00081 [221-241] 2.800005042611e-30 PR00081
PR00081   GDHRDH
IPR016040 NAD(P)-binding domain (Domain)
[5-257] 1.29999999999998e-72 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR020904 Short-chain dehydrogenase/reductase, conserved site (Conserved_site)
[153-181] PS00061
PS00061   ADH_SHORT
SignalP
[1-28] 0.671 Signal
Bacteria, Gram-negative   
[1-28] 0.598 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Griseo_00100 Griseo_00100   Griseo_00120 Griseo_00120
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