Griseo_00110 Griseo_00110   Griseo_00130 Griseo_00130

Griseo_00120 : CDS information

close this sectionLocation

Organism
StrainJP95
Entry nameGriseorhodin A
Contig
Start / Stop / Direction11,774 / 13,024 / + [in whole cluster]
11,774 / 13,024 / + [in contig]
Location11774..13024 [in whole cluster]
11774..13024 [in contig]
TypeCDS
Length1,251 bp (416 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productputative cytochrome P450
Product (GenBank)putative cytochrome P450 GrhO3
Gene
Gene (GenBank)grhO3
EC number
Keyword
  • epoxidation
Note
Note (GenBank)
Reference
ACC
PmId
[12323376] A gene cluster from a marine Streptomyces encoding the biosynthesis of the aromatic spiroketal polyketide griseorhodin A. (Chem Biol. , 2002)
[19175308] Cleavage of four carbon-carbon bonds during biosynthesis of the griseorhodin a spiroketal pharmacophore. (J Am Chem Soc. , 2009)
comment
[PMID:12323376](2002)
griseorhodin A生合成gene clusterのクローニング、シークエンス、異種性発現。

GrhO3: Cytochrome P450

配列解析のみ。
grhO3 and grhO4によってコードされるputative P450/ferredoxin pairがepoxide形成するようだと提唱。

--
[PMID:19175308](2009)
grhO3: cytochrome P450

grhO7 or grhO3 deletion mutantはdideoxygriseorhodin Cを蓄積。
dideoxygriseorhodin Cは、epoxide部分のないgriseorhodin A。
よってGrhO7 と GrhO3はepoxidation形成に一緒に関与する。
この結果から2つのepoxidation機構を提唱。

1)GrhO3とGrhO7は複合体を形成し、olefinic中間体(二重結合あり)を介してdideoxygriseorhodin Cをepoxidizeする。

2)GrhO3が単結合からepoxideを直接生成し、GrhO7は消去的副反応から生じるolefinic shunt産物の還元的再利用を担う。
Related Reference
ACC
B3XWK3
PmId
[18640605] Increase in pladienolide D production rate using a Streptomyces strain overexpressing a cytochrome P450 gene. (J Biosci Bioeng. , 2008)
comment
Blast 12th, id53%, 1e-109
Streptomyces sp. A-1544_psmA
Cytochrome P450 monooxygenase

Pladienolide Bの16-hydroxylated derivativeであるpladienolide D産生を促進するための株Streptomyces bungoensis A-1544から分離したpladienolide B 16-hydroxylase PsmA。
PsmAの過剰発現で15倍のpladienolide Dを得た。
ferredoxin_psmBが一緒でないと変換率はぐっと低くなる。
ACC
Q9EW92
NITE
Pimar_00180
PmId
[15228385] Characterization of the polyene macrolide P450 epoxidase from Streptomyces natalensis that converts de-epoxypimaricin into pimaricin. (Biochem J. , 2005)
comment
Blast list外, id31%, 4e-49
Streptomyces natalensis_pimD
PimD protein
[DoBISCUIT]epoxidase(cytochrome P450)

N-terminal His6 affinity tagを持つPimDをE.coliで過剰発現させて4,5-de-epoxypimaricin→pimaricinへの酵素活性など確認。

close this sectionSequence

selected fasta
>putative cytochrome P450 [putative cytochrome P450 GrhO3]
MTDTLDEPQTLADGAEDAPAYPVKRTCPYRMPPGYEELREKGPISRVTLWNGRTAWLVTG
NDLGRRLFPDARLSSDVLDPRFPLLAPRIEAQRQQAAAPPLVGVDDPVHARQRRMVLPSF
GIRQINALRPEIQKYADDLLDTMLAKGPGVTVDLLTEYALPMPSAVICMLLGVPYEDHHY
FDERSRHVLSSSGEEQAAQAQQAFTEILAYLDDLIVRKQAEPGDTLLDELIARQLEEGKV
DRQELAMIATVLLVSGHETTSNMIALSTMALLADPDQLAALRADESLMPRAVDELMRFSS
IGDMLMRVAKEDIEIEGHLIRAGDGVILSTMLMNRDPGAFERPDELDIRRPAGRHVAFGY
GIHQCIGQNLARAEMEIALATLFRRVPTLKLAVPAEQVPVNAPFVLQGVSELPVTW
selected fasta
>putative cytochrome P450 [putative cytochrome P450 GrhO3]
ATGACCGACACACTCGACGAGCCGCAGACGCTGGCGGACGGCGCCGAGGACGCGCCCGCC
TACCCCGTCAAACGCACCTGCCCCTACCGGATGCCGCCCGGCTACGAGGAACTCCGGGAG
AAGGGCCCCATCTCCCGGGTGACCCTCTGGAACGGACGGACGGCCTGGCTGGTCACCGGC
AACGACCTCGGCCGCAGGCTGTTCCCGGACGCCCGGCTCTCCTCGGACGTCCTGGACCCG
CGCTTCCCGCTGCTCGCGCCCCGGATCGAGGCGCAGCGGCAGCAGGCCGCCGCGCCGCCG
CTGGTCGGCGTCGACGACCCGGTCCACGCCCGTCAGCGCCGCATGGTGCTGCCGAGCTTC
GGCATCCGCCAGATCAACGCGCTGCGCCCGGAGATCCAGAAGTACGCCGACGACCTGCTG
GACACGATGCTGGCCAAGGGTCCGGGCGTCACCGTCGACCTGCTCACCGAGTACGCGCTA
CCGATGCCGTCCGCCGTGATCTGCATGCTGCTCGGCGTCCCGTACGAGGACCACCACTAC
TTCGACGAGCGCTCGCGCCACGTCCTGTCCTCGTCCGGCGAGGAGCAGGCGGCGCAGGCG
CAGCAGGCGTTCACCGAGATCCTGGCCTACCTGGACGACCTGATCGTCCGCAAGCAGGCC
GAGCCCGGCGACACCCTCCTCGACGAGCTCATCGCCCGCCAGTTGGAGGAGGGCAAGGTC
GACCGCCAGGAGCTGGCGATGATCGCCACCGTGCTGCTGGTCTCCGGGCACGAGACGACC
TCGAACATGATCGCCCTCTCCACGATGGCGCTGCTGGCCGACCCGGACCAGTTGGCGGCC
CTGCGCGCCGACGAGTCCCTGATGCCCCGCGCCGTCGACGAGCTGATGCGGTTCTCCTCC
ATCGGCGACATGCTGATGCGCGTGGCCAAGGAGGACATCGAGATCGAGGGCCACCTCATC
CGGGCCGGCGACGGGGTGATCCTCTCGACCATGCTGATGAACCGCGACCCCGGGGCCTTC
GAGCGCCCCGACGAGCTGGACATCCGCCGGCCCGCGGGCCGCCACGTCGCGTTCGGCTAC
GGCATCCACCAGTGCATCGGGCAGAACCTGGCCCGCGCCGAGATGGAGATCGCGCTGGCC
ACCCTGTTCCGCCGGGTGCCGACGCTGAAGCTCGCGGTCCCCGCCGAGCAGGTGCCCGTC
AACGCGCCGTTCGTCCTCCAGGGAGTCTCCGAACTCCCCGTCACCTGGTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001128 Cytochrome P450 (Family)
[255-272] 5.20000089003336e-07 PR00385 [290-301] 5.20000089003336e-07 PR00385 [356-365] 5.20000089003336e-07 PR00385 [365-376] 5.20000089003336e-07 PR00385
PR00385   P450
[107-385] 1.1e-27 PF00067
PF00067   p450
[28-416] 1.3000049540733e-93 SSF48264
SSF48264   Cytochrome_P450
[27-416] 2.99999999999998e-103 G3DSA:1.10.630.10
G3DSA:1.10.630.10   Cyt_P450
IPR002397 Cytochrome P450, B-class (Family)
[104-115] 3.59998852907783e-53 PR00359 [153-169] 3.59998852907783e-53 PR00359 [170-185] 3.59998852907783e-53 PR00359 [209-231] 3.59998852907783e-53 PR00359 [290-301] 3.59998852907783e-53 PR00359 [307-334] 3.59998852907783e-53 PR00359 [335-350] 3.59998852907783e-53 PR00359 [356-365] 3.59998852907783e-53 PR00359 [365-376] 3.59998852907783e-53 PR00359
PR00359   BP450
IPR017972 Cytochrome P450, conserved site (Conserved_site)
[358-367] PS00086
PS00086   CYTOCHROME_P450
SignalP No significant hit
TMHMM No significant hit
Griseo_00110 Griseo_00110   Griseo_00130 Griseo_00130
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