Indano_00100 Indano_00100   Indano_00120 Indano_00120

Indano_00110 : CDS information

close this sectionLocation

Organism
StrainNRRL 8167
Entry nameIndanomycin
Contig
Start / Stop / Direction13,164 / 15,890 / + [in whole cluster]
13,164 / 15,890 / + [in contig]
Location13164..15890 [in whole cluster]
13164..15890 [in contig]
TypeCDS
Length2,727 bp (908 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
Productputative LuxR family transcriptional regulator
Product (GenBank)LuxR family transcriptional regulator
Gene
Gene (GenBank)idmG
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[19301315] Analysis of the indanomycin biosynthetic gene cluster from Streptomyces antibioticus NRRL 8167. (Chembiochem. , 2009)
comment
indanomycin biosynthetic gene clusterに関する文献。

idmG(908a.a): LuxR-family transcriptional regulator

idmG自体の機能実験は無いが、相同性よりLuxR-family transcriptional regulatorであるとしている。
Related Reference
ACC
Q9S0N8
NITE
Aver_00010
PmId
[10449723] Organization of the biosynthetic gene cluster for the polyketide anthelmintic macrolide avermectin in Streptomyces avermitilis. (Proc Natl Acad Sci U S A. , 1999)
[19148632] Characterization of a regulatory gene, aveR, for the biosynthesis of avermectin in Streptomyces avermitilis. (Appl Microbiol Biotechnol. , 2009)
[20012992] The pathway-specific regulator AveR from Streptomyces avermitilis positively regulates avermectin production while it negatively affects oligomycin biosynthesis. (Mol Genet Genomics. , 2010)
comment
<AveR protein, Streptomyces avermitilis, BLAST 7; id=39%>
[Aver_00010]LuxR family transcriptional regulator
avermectinにはpositiveに、oligomycinにはnegativeに作用するregulator.

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[PMID:10449723](1999)
avermectin生合成gene clusterの報告。

AveR: positive regulator

aveR mutantsの表現型、DNA segmentsを導入することによるその相補特性、helix-turn-helix motifを持つタンパクへの配列類似から、AveRがpotential regulatory functionを持つことが示される。

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[PMID: 19148632](2009)abstract
aveR 不活化mutantは、avermectin中間体をいかなるavermectin派生体にも変換できない。よってAveRは、avermectinの生合成遺伝子と修飾遺伝子の両方の発現を調節するpositive regulatorであることが示唆された。
また、多量のaveRはavermectinの完全な損失をもたらすことから、aveRには最高許容限界濃度がある。

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[PMID: 20012992](2010)abstract
aveR deletion mutantはavermectin産生を消失し、oligomycin産生が増える。この表現型はaveR geneのsingle copyによって相補された。

AveRのC末HTH domainの除去はavermectin生合成を消失。
ave clusterとolm clusterの両方のpredicted promoter regionsがAveRのtarget sitesであり、AveRのDNA-binding activityはHTH domainに依存的である。
aveの構造遺伝子の転写はAveRに依存的であるが、olmの構造遺伝子とputative pathway-specific regulatory genesの転写はaveR mutantsで増える。

avermectin PKS_AVES1をコードするaveA1の転写がAveRによって活性化される。
aveRの過剰発現でavermectin産生増加。

close this sectionSequence

selected fasta
>putative LuxR family transcriptional regulator [LuxR family transcriptional regulator]
MILVNREAELGLLKSGLAECAAGRAKMIVVEGGVGCGKSELLDTFTEYATEQGAAVLRGM
GIRSASDQPLALLRQLLGQVPGWVPSEPGVGEQEFGEALHRLASRSPVVVCVDDAQYGDS
RSLHHLLQAAGFTRSARILLVFGDSLHLPRRDPVIKTELLRQSNSLRIRLGPLTKQGTAA
LLAAQGGDAQDRPLVDRLHFITAGNPLLLRALLEECRPATADSGIADKIEPMEGGLYGQA
VLASLYRSGPAALELGHALAVLDEPRAHAILPRLVDQPSALIDQNLQALTAAGMIAGTAF
RHQAAVATVLADLAPDRRTALHSWVAVALHSAGYPASVVARHLLKARSAAQPWELIALRN
AAEEALAEDDAVLAIDCLELAHEACPDPVQRIEIKMRLALITWRTNPAAAEYRHLPELLS
AMRRGELSPTSLGMLGKLLSAHGRIVEAMEVLRLSEDASCDVPRPVPGIHPTIASMWVRY
GFHGVDFPDSGQRGGAAQGPASDSGSLVNGSVPGIPLLRKSAEDVLIAAQDWSGSAESFL
EMCSLTDSMVAPVCSALKALLYSDQVEAAAQWCAKILDEATRRNIPGWQGVLAVVQGMIA
LRQGKLEEAVASASMALEVVPERNGSVLVCGTSSILAMAYTEMGRYDAAVRLLDQPVSQD
WFDSVYWLGYLRARGHFHLATNRSHAALSDFLRIGNLAGSWGIDHPMLVSWRTDAAQAWL
QLGEPSQAQQLLDAEVSQEFPGYGRLRGITLRLQAAIAPLTDRVQLLGRAVEEFDAGNDR
LELAKALADLSEAHEALGNQTRADLVRRKGRHLAAECGADPLYRRMAAGGRNRMVAADSE
SGSVRTEPKPGVDLTEAEYRVASLASYGHTNREISQQLYITVSTVEQHLTRIYQKLRITR
RQEIPVDL
selected fasta
>putative LuxR family transcriptional regulator [LuxR family transcriptional regulator]
ATGATACTGGTCAATCGTGAAGCGGAACTTGGGCTACTGAAGTCCGGACTCGCGGAATGC
GCAGCCGGGCGGGCCAAGATGATCGTCGTGGAAGGCGGCGTGGGCTGCGGCAAGAGCGAG
TTGCTGGACACGTTCACGGAATATGCCACCGAACAGGGGGCGGCGGTCCTCAGAGGGATG
GGCATACGCTCCGCATCCGATCAGCCCCTCGCCCTGCTACGCCAGTTACTGGGGCAGGTG
CCGGGCTGGGTTCCCTCCGAACCCGGCGTCGGGGAGCAGGAGTTCGGCGAGGCCCTCCAC
CGGCTGGCGAGCCGGAGCCCCGTGGTCGTCTGTGTGGACGACGCGCAGTACGGCGACAGC
CGGTCGCTTCACCACTTGTTGCAAGCGGCGGGCTTCACCCGATCCGCCCGGATCCTCCTC
GTGTTCGGCGACAGCCTGCACCTCCCGCGCCGGGACCCGGTGATCAAGACGGAGCTGCTG
CGGCAGTCCAACTCACTGCGGATCCGGCTGGGTCCGCTGACCAAGCAGGGCACCGCCGCG
CTGCTCGCCGCGCAGGGCGGCGACGCACAGGACCGTCCCCTCGTGGACCGGCTGCACTTC
ATCACCGCCGGAAACCCGCTGCTCCTGCGGGCGCTGCTGGAGGAATGCCGTCCCGCCACC
GCAGACTCCGGGATCGCGGACAAGATCGAGCCCATGGAGGGCGGACTCTACGGACAGGCG
GTCCTCGCCAGCCTGTACCGCAGTGGTCCGGCCGCCCTGGAGCTGGGGCACGCGCTCGCC
GTCCTGGACGAGCCGAGGGCCCACGCGATCCTGCCCAGGCTGGTCGACCAGCCCTCGGCG
CTGATCGACCAGAACCTCCAGGCCCTCACCGCGGCCGGCATGATCGCGGGCACCGCGTTC
CGGCACCAGGCCGCCGTCGCGACCGTCCTGGCCGACCTGGCGCCCGACCGCCGTACCGCC
CTGCACTCCTGGGTGGCCGTGGCCCTGCACTCCGCCGGATATCCGGCGTCGGTGGTCGCG
CGCCACCTGCTCAAAGCGCGCAGCGCCGCCCAGCCCTGGGAGCTCATCGCGCTGCGCAAC
GCCGCCGAGGAGGCACTCGCCGAGGACGATGCCGTCCTGGCCATCGACTGTCTCGAACTG
GCTCATGAGGCCTGCCCTGACCCGGTCCAACGGATCGAGATCAAGATGCGGCTGGCGCTG
ATCACCTGGCGCACCAACCCCGCGGCCGCGGAGTACCGCCATCTCCCTGAACTCCTCAGC
GCCATGCGGCGGGGCGAGCTGTCGCCGACCAGTCTCGGCATGCTCGGCAAGCTGCTGTCC
GCGCACGGCCGCATAGTGGAGGCGATGGAGGTCCTCCGGCTGAGCGAGGACGCGTCGTGC
GACGTGCCCCGGCCCGTCCCCGGCATCCACCCCACCATCGCCTCGATGTGGGTGCGCTAC
GGGTTCCACGGCGTGGACTTCCCCGACAGCGGCCAGCGCGGCGGCGCGGCGCAGGGGCCG
GCCTCCGACTCCGGCTCCCTGGTCAACGGTTCCGTCCCCGGCATACCGCTGCTGCGGAAG
TCCGCGGAGGACGTGCTCATCGCCGCGCAGGACTGGAGCGGGTCCGCGGAGAGTTTCCTG
GAGATGTGCTCCCTCACCGACTCGATGGTGGCCCCGGTGTGCAGCGCGCTCAAGGCGCTG
CTCTACTCCGACCAGGTCGAGGCGGCGGCGCAGTGGTGCGCGAAGATCCTGGATGAGGCC
ACCCGCCGCAACATCCCGGGCTGGCAGGGGGTGTTGGCCGTCGTCCAGGGGATGATCGCG
CTGCGGCAGGGCAAGTTGGAGGAGGCCGTCGCCAGCGCGTCCATGGCGCTGGAAGTGGTC
CCCGAGCGCAACGGCAGCGTGCTGGTCTGTGGTACCTCCTCCATCCTGGCCATGGCATAC
ACCGAGATGGGGCGCTACGACGCCGCGGTACGCCTGCTCGACCAGCCGGTCTCCCAGGAC
TGGTTCGACAGCGTGTACTGGCTCGGCTACCTGCGGGCGCGGGGGCACTTCCATCTGGCG
ACGAACCGCTCGCATGCCGCTCTGTCGGACTTCCTGAGAATCGGGAACCTGGCGGGCAGC
TGGGGGATCGACCACCCCATGCTGGTGTCGTGGCGCACCGACGCGGCCCAGGCGTGGCTC
CAGCTCGGGGAGCCGTCCCAGGCCCAGCAGCTGCTCGACGCCGAAGTCAGCCAGGAGTTC
CCCGGGTACGGCCGGCTTCGGGGGATCACCCTGAGGCTGCAGGCCGCGATCGCGCCGCTG
ACCGACCGCGTACAGCTGCTCGGTCGGGCCGTCGAGGAGTTCGACGCAGGCAATGACCGG
CTGGAACTGGCCAAGGCGCTGGCCGACCTCAGTGAGGCGCATGAGGCGCTGGGCAACCAG
ACCCGCGCGGATCTGGTCAGGCGCAAGGGCCGGCATCTCGCCGCGGAATGCGGGGCGGAT
CCGCTGTACCGGCGCATGGCCGCCGGCGGACGGAACAGGATGGTCGCCGCGGATTCGGAA
TCCGGCTCGGTGCGAACCGAACCGAAACCCGGAGTGGATCTGACCGAAGCGGAGTACCGG
GTGGCCTCGCTGGCGTCCTACGGTCATACCAATCGCGAGATATCCCAGCAACTGTATATA
ACCGTCAGTACCGTCGAGCAGCATCTCACGCGGATATACCAGAAACTCCGGATAACGCGA
CGCCAGGAGATACCGGTCGATCTGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000792 Transcription regulator LuxR, C-terminal (Domain)
[851-908] 4.90001027782405e-18 SM00421
SM00421   HTH_LUXR
[847-908] PS50043
PS50043   HTH_LUXR_2
[868-895] PS00622
PS00622   HTH_LUXR_1
[853-904] 1.2e-08 PF00196
PF00196   GerE
[854-868] 5.20000089003336e-08 PR00038 [868-884] 5.20000089003336e-08 PR00038 [884-896] 5.20000089003336e-08 PR00038
PR00038   HTHLUXR
IPR011990 Tetratricopeptide-like helical (Domain)
[561-861] 9.20000000000002e-20 G3DSA:1.25.40.10
G3DSA:1.25.40.10   TPR-like_helical
IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain)
[862-904] 8.5e-16 G3DSA:1.10.10.10
G3DSA:1.10.10.10   Wing_hlx_DNA_bd
IPR016032 Signal transduction response regulator, C-terminal effector (Domain)
[832-904] 6.20000124972815e-12 SSF46894
SSF46894   Bipartite_resp_reg_C-effector
SignalP No significant hit
TMHMM No significant hit
Indano_00100 Indano_00100   Indano_00120 Indano_00120
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