Ncarz_00340 : CDS information

Location

Organism	Streptomyces carzinostaticus
Strain	ATCC 15944
Entry name	Neocarzinostatin
Contig	AY117439
Start / Stop / Direction	59,568 / 58,474 / - [in whole cluster] 59,568 / 58,474 / - [in contig]
Location	complement(58474..59568) [in whole cluster] complement(58474..59568) [in contig]
Type	CDS
Length	1,095 bp (364 aa)

Annotation

Category	5.3 uncharacterized protein (biosynthesis involved)
Product	hypothetical protein
Product (GenBank)	putative transporter
Gene	ncsE5 unbU
Gene (GenBank)
EC number
Keyword	enediyne core
Note
Note (GenBank)
Reference	ACC Q83U30 PmId [15797213] The neocarzinostatin biosynthetic gene cluster from Streptomyces carzinostaticus ATCC 15944 involving two iterative type I polyketide synthases. (Chem Biol. , 2005) [12536216] A genomics-guided approach for discovering and expressing cryptic metabolic pathways. (Nat Biotechnol. , 2003) comment Neocarzinostatin(NCS)生合成遺伝子クラスターの報告。 ncsE5: unknown protein ncsE-ncsE11とncsF1-ncsF2が、NCS enediyne core biosynthesisに関与するとしている。このORFについて機能解析されていない。 [PMID:12536216] warhead cassette(UNBL-UNBV-UNBU-PKSE-TEBC)はactinomyceteにおいて全てのenediyne生合成遺伝子座で保存されているが、機能は未知のままである。enediyne warheadのformation, stabilization, or transport に関与しているのではないかと推測している。 UNBUは、7 or 8のmembrane-spanning alpha helicesを持つ内在性膜蛋白質だと推定している。このclusterでもwarhead cassetteはNcarz_00320-Ncarz_00360で保存されている。

Sequence

>hypothetical protein [putative transporter]
MTMSAAQPLSQTAGPRHNPKVVTALRRFAISISVLNIVGYTVLGFEQPWLWPFIALATAY
TVELTLEAVGARAEKRATRFRGGGFRGLMEFLYPAHITALAVNMLTYVNDQVWVMVFGVV
VAVGAKWVLRAPVRGRMRHFMNPSNLGIAVILLLFPWASIAPPYHFTEYVDGGIDWLVPA
VILTLGTMLNAKLTERMWLIVAWVGGFAAQAVVRGLLFGTSIPAALAMMTGVAFVLFTNY
MITDPGTTPSSKWGQIAFGGGVAAAYGLLTALSVAYGIFFATALVCGVRGGFLWISDIVS
RRRTEEALAVAAVGLTSEKPSVERTPIEHAETLPCVNGCAEGACSCSGNPKDSEADDRRI
VVSA

>hypothetical protein [putative transporter]
GTGACCATGTCCGCAGCACAGCCCCTGTCCCAGACCGCGGGACCGCGCCACAACCCGAAG
GTCGTCACCGCCCTCCGCCGCTTCGCGATCTCGATCTCCGTACTCAACATCGTCGGCTAC
ACCGTACTGGGCTTCGAACAGCCCTGGCTCTGGCCCTTCATCGCGCTGGCGACGGCCTAC
ACCGTGGAGCTCACGCTCGAGGCCGTCGGTGCACGCGCCGAGAAGCGAGCGACTCGCTTC
CGCGGGGGCGGCTTCCGAGGGCTGATGGAATTCCTCTACCCCGCACACATCACGGCCCTC
GCGGTGAACATGCTCACCTACGTCAACGACCAGGTGTGGGTCATGGTGTTCGGCGTCGTG
GTGGCGGTAGGCGCCAAGTGGGTGCTGCGGGCCCCGGTGAGGGGCCGCATGCGCCACTTC
ATGAACCCCTCGAATCTCGGCATCGCGGTCATCCTGCTGTTGTTCCCGTGGGCGTCGATC
GCCCCGCCGTACCACTTCACCGAGTACGTGGACGGCGGCATTGACTGGCTCGTGCCGGCC
GTCATCCTCACGCTCGGCACCATGCTCAACGCGAAGCTCACCGAACGCATGTGGCTCATC
GTCGCGTGGGTCGGCGGGTTCGCCGCGCAGGCGGTCGTGCGAGGGCTGTTGTTCGGCACG
TCGATTCCTGCGGCCCTCGCGATGATGACCGGTGTGGCGTTCGTACTCTTCACGAACTAC
ATGATCACGGACCCCGGCACCACGCCGTCCTCCAAGTGGGGCCAGATCGCCTTCGGGGGC
GGTGTCGCCGCCGCGTACGGCCTCCTGACGGCGCTGAGTGTCGCCTACGGGATCTTCTTC
GCCACCGCTCTGGTCTGCGGGGTCCGCGGTGGCTTCCTGTGGATCAGCGACATCGTCTCC
CGCAGGCGCACCGAGGAGGCCCTGGCCGTTGCCGCGGTCGGCCTGACCTCCGAGAAGCCC
TCCGTCGAGCGCACCCCGATCGAGCACGCGGAAACACTCCCGTGCGTGAACGGCTGCGCC
GAAGGGGCCTGCTCCTGCTCCGGGAACCCGAAGGACAGCGAGGCGGACGACCGCAGGATC
GTGGTGTCGGCATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro	No significant hit
SignalP	No significant hit
TMHMM	Transmembrane 8

Ncarz_00330 Ncarz_00350