Ncarz_00350 : CDS information

Location

Organism	Streptomyces carzinostaticus
Strain	ATCC 15944
Entry name	Neocarzinostatin
Contig	AY117439
Start / Stop / Direction	61,475 / 59,565 / - [in whole cluster] 61,475 / 59,565 / - [in contig]
Location	complement(59565..61475) [in whole cluster] complement(59565..61475) [in contig]
Type	CDS
Length	1,911 bp (636 aa)

Annotation

Category	5.3 uncharacterized protein (biosynthesis involved)
Product	hypothetical protein
Product (GenBank)	unknown
Gene	ncsE4 unbV
Gene (GenBank)
EC number
Keyword	enediyne core
Note
Note (GenBank)
Reference	ACC Q83UM5 PmId [15797213] The neocarzinostatin biosynthetic gene cluster from Streptomyces carzinostaticus ATCC 15944 involving two iterative type I polyketide synthases. (Chem Biol. , 2005) [12536216] A genomics-guided approach for discovering and expressing cryptic metabolic pathways. (Nat Biotechnol. , 2003) comment Neocarzinostatin(NCS)生合成遺伝子クラスターの報告。 ncsE4: unknown protein ncsE-ncsE11とncsF1-ncsF2が、NCS enediyne core biosynthesisに関与するとしている。このORFについて機能解析されていない。 [PMID:12536216] warhead cassette(UNBL-UNBV-UNBU-PKSE-TEBC)はactinomyceteにおいて全てのenediyne生合成遺伝子座で保存されているが、機能は未知のままである。enediyne warheadのformation, stabilization, or transport に関与しているのではないかと推測している。 UNBVの構造解析は、N末端シグナル配列と共にsecreted proteinを持っていた。このclusterでもwarhead cassetteはNcarz_00320-Ncarz_00360で保存されている。
Related Reference	ACC Q84HJ0 NITE Dynm_00160 PmId [18328078] The biosynthetic genes encoding for the production of the dynemicin enediyne core in Micromonospora chersina ATCC53710. (FEMS Microbiol Lett. , 2008) comment Micromonospora chersina_dynU14 DynU14 dynemicin生合成遺伝子 dynU14欠損株を作製し、培養液中の産物をHPLCにて検出。dynU14欠損株はdynemicin Aが検出出来なかったことから、dynU14はdynemicin Aに必須であるとしている。 dynU15, dynU14, dynT3, dynE8, dynE7は、minimal enediyne cassetteとして知られている遺伝子と推定している。dynU15, dynU14, dynT3はconserved unknown proteinsだと記述あり。

Sequence

>hypothetical protein [unknown]
MTMAKNWLRRNSPGIVALTLMASVFYVVRLPEPSAADVRESAADFAFEPMTIAMPGGFPT
QKIRQVNKAYEHIDAWISSVGAGIALNDMDGDGLSNDLCLTDPRIDQAVVTPAPSRGKAY
EPFALDAAPLGISDTMAPMGCVPGDFNEDGAIDLLVYYWGRTPVIFQNEGGRGEPLTASS
FTPTELLPGKPGPRYTGPLWNSNTAAVADFDGDGHDDIYIGNYFPDSPVLDPSKNGDVTM
NDSLSHAQNGGGGHFFRWTESGFEKTDDAIPQGLNKGWSLGASAADLDGDRLPEIFLAHD
FGTSALLHNTSRPGRIEFREVKAVHSGTVPKSKEIGRSSFKGMGVDFGDLDHDGLYDMFV
SNITTSFGIQESNFAFINKAGDKADLRSRFENGEAPYRDESTDLGLAWSGWGWDVKMGDF
DNNGDLEITQALGFVKGKNNRWPQLQELATSNDALVANPTWWPNVRQGDDLAGSQRMRFF
AKDQDTGRYINLSTALGLGDPVPTRGIATGDVDGDGRLDIAVARQWDEPVFYRNTAPEPG
SWLELVFTHPDGAPVVGAEVRVELPDGSKRVARVDGGGGHSGKRSTDIHIGLGEEAQGEV
SGTVTWRDREGDVHEQEVRLAPGRHSFELGSQVKEK

>hypothetical protein [unknown]
GTGACCATGGCGAAGAACTGGCTACGCAGGAATTCTCCGGGAATCGTCGCGCTCACCCTG
ATGGCGAGCGTCTTCTACGTCGTTCGCCTCCCTGAACCGTCTGCCGCCGATGTCAGGGAA
TCGGCAGCCGACTTCGCCTTCGAGCCGATGACCATAGCCATGCCGGGAGGATTTCCCACA
CAGAAGATCAGACAGGTCAACAAGGCTTACGAGCACATCGACGCCTGGATTTCATCGGTC
GGCGCCGGCATCGCCCTCAATGACATGGACGGCGACGGCCTGTCCAATGATCTGTGCCTG
ACCGACCCCAGGATCGACCAGGCCGTGGTGACCCCGGCTCCCTCGCGCGGCAAGGCCTAC
GAACCGTTCGCACTCGATGCGGCCCCCCTGGGAATCAGCGACACCATGGCTCCGATGGGG
TGCGTACCCGGTGACTTCAACGAGGACGGCGCCATCGACCTGCTCGTCTACTACTGGGGC
CGCACCCCTGTGATCTTCCAGAACGAAGGTGGCCGTGGCGAGCCACTCACCGCTTCCTCG
TTCACGCCCACGGAACTGCTACCGGGTAAACCCGGCCCGCGGTACACGGGTCCGCTGTGG
AACAGCAACACAGCCGCCGTCGCCGACTTCGACGGCGACGGACACGACGACATCTACATC
GGCAACTACTTCCCCGACAGCCCGGTCCTCGACCCGTCCAAGAACGGCGACGTCACCATG
AACGACTCGCTGTCGCACGCCCAGAACGGCGGTGGTGGTCACTTCTTCCGCTGGACCGAG
TCCGGTTTCGAGAAGACGGACGATGCCATACCGCAGGGCCTCAACAAGGGATGGTCACTC
GGCGCGTCGGCCGCGGACCTTGACGGCGACCGTCTTCCTGAGATCTTCCTCGCCCATGAC
TTCGGGACCTCGGCGCTGTTGCACAACACCTCGCGGCCGGGCCGGATCGAGTTCCGCGAG
GTCAAAGCGGTCCACTCCGGCACCGTTCCCAAGTCCAAGGAGATCGGACGCAGCTCCTTC
AAGGGGATGGGTGTCGACTTCGGTGACCTGGACCACGACGGCCTGTACGACATGTTCGTC
AGCAACATCACGACATCGTTCGGGATCCAGGAGTCGAACTTCGCCTTCATCAACAAGGCC
GGCGACAAGGCCGACCTGCGGTCCCGCTTCGAGAACGGCGAGGCGCCCTACAGGGACGAG
TCGACCGACCTCGGCCTGGCCTGGTCCGGCTGGGGCTGGGACGTGAAGATGGGCGATTTC
GACAACAACGGCGATCTTGAGATCACCCAGGCGCTCGGTTTCGTCAAGGGCAAGAACAAC
CGCTGGCCGCAGTTGCAGGAACTCGCCACGTCCAACGACGCGCTGGTCGCCAACCCCACC
TGGTGGCCGAACGTCAGGCAGGGAGATGACCTCGCCGGCAGCCAGCGGATGCGGTTCTTC
GCCAAGGACCAGGACACCGGCCGCTACATCAACCTCTCCACGGCGCTGGGCCTGGGGGAT
CCTGTTCCGACCCGTGGCATCGCGACCGGTGACGTGGACGGCGACGGCCGCCTCGACATC
GCAGTCGCCCGCCAGTGGGACGAGCCCGTCTTCTACCGCAACACGGCCCCCGAGCCCGGC
TCCTGGCTGGAACTCGTCTTCACGCACCCCGACGGTGCTCCGGTGGTCGGAGCCGAAGTC
CGCGTCGAGCTGCCCGACGGGAGCAAGAGGGTCGCCCGCGTCGACGGGGGCGGTGGCCAC
TCGGGCAAACGAAGTACCGATATCCACATCGGCCTGGGCGAGGAGGCCCAGGGCGAGGTC
TCAGGGACGGTCACCTGGCGCGACCGCGAAGGTGACGTCCACGAGCAGGAAGTGAGGCTG
GCGCCGGGCAGGCACAGCTTCGAGCTCGGCAGCCAGGTCAAGGAGAAGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR011519 ASPIC/UnbV (Domain) PF07593 UnbV_ASPIC IPR013517 FG-GAP repeat (Repeat) PF01839 FG-GAP
SignalP	Eukaryota Bacteria, Gram-positive Bacteria, Gram-negative
TMHMM	No significant hit

Ncarz_00340 Ncarz_00360