Oxzol_00080 Oxzol_00080   Oxzol_00100 Oxzol_00100

Oxzol_00090 : CDS information

close this sectionLocation

Organism
StrainJA3453
Entry nameOxazolomycin
Contig
Start / Stop / Direction16,523 / 15,546 / - [in whole cluster]
16,523 / 15,546 / - [in contig]
Locationcomplement(15546..16523) [in whole cluster]
complement(15546..16523) [in contig]
TypeCDS
Length978 bp (325 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.1 modification addition of extender units
Productputative acyltransferase
Product (GenBank)OzmC
Gene
Gene (GenBank)ozmC
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[16707707] Utilization of the methoxymalonyl-acyl carrier protein biosynthesis locus for cloning the oxazolomycin biosynthetic gene cluster from Streptomyces albus JA3453. (J Bacteriol. , 2006)
[20406823] Oxazolomycin biosynthesis in Streptomyces albus JA3453 featuring an "acyltransferase-less" type I polyketide synthase that incorporates two distinct extender units. (J Biol Chem. , 2010)
comment
[PMID:16707707]
methoxymalonyl-ACP生合成遺伝子の同定とクローニング

OzmB, OzmC, OzmD, OzmE, OzmF, and OzmGが、methoxymalonyl-ACP生合成遺伝子であることを同定し、これらがoxazolomycin productionに必須であることを示した。
ozmCの不活性化実験や相補実験からはOZM生合成に必須であることが推測されたが、機能は不明なまま。


[PMID:20406823]
oxazolomycin生合成遺伝子クラスターの報告。

ozmC: Acyltransferase

OzmCはOZM AT-less type I PKSのmodule 10に対してmethoxymalonyl-ACP extenderをloadingするdiscrete ATの候補遺伝子だと推測している。module10を除くAT-less PKS moduleすべてのmalonyl-CoA extender unitのloadingにはOzmMが関与すると証明している。

本文中でmethoxmalonyl-ACP extender unitをloadingする既知のtypeI PKS全てとOzmCは相同性を示さないこと、Streptomyces peucetiusのDaunorubicin-doxorubicin polyketide synthase(dpsC)と相同性を示している。dpsCとの相同性は低い(25% identities)。

このORFについての機能解析実験はされていない。
Family/Domain
FD
IPR013747
comment
[IPR013747] 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C-terminal (Domain)

close this sectionSequence

selected fasta
>putative acyltransferase [OzmC]
MTTAAGDIGIGAIHCLLPDERVAVADLPELARLTDEELRFAEQAGIKEVGVFPDTEPTEL
AARACRELLAAHPGVPDLMLHIGSRAPDVLIGSDSGKIAHLAGLTGVFPFTVDGLGCAGS
SAAWGLGRDLLRGDPSRESVLLTYGSRPTAADRVRRPVTVIGDGAFAMTLVRGGRPLLRA
HRMEADSSFHDLFKVEYKNSPWSQWREVCDDNDRYSFELAMHSRTRLSKLVDQVLADAGI
GKDDVAATLMQNVTSSAFDFYKATLGLPIHPVCATHLAELGHLGPMDVVLNLDRLLATGE
VGPGGHVLVLSNSPVAAWTVTLWEV
selected fasta
>putative acyltransferase [OzmC]
ATGACGACGGCTGCCGGAGACATCGGAATAGGCGCGATCCACTGCTTGCTGCCCGACGAG
CGCGTGGCCGTCGCCGACCTGCCCGAGCTGGCCAGGCTCACCGACGAGGAGCTGAGGTTC
GCCGAGCAGGCCGGGATCAAGGAGGTCGGTGTCTTCCCCGACACCGAGCCGACCGAACTG
GCGGCGCGGGCCTGCCGGGAACTGCTCGCCGCGCATCCGGGCGTCCCCGACCTGATGCTG
CACATCGGCTCCCGGGCACCGGACGTGCTGATCGGCTCGGACTCGGGCAAGATCGCCCAC
CTGGCCGGGCTCACCGGCGTCTTCCCCTTCACCGTCGACGGCCTGGGCTGTGCGGGCTCC
TCCGCCGCCTGGGGCCTCGGCAGGGACCTGCTCCGCGGCGATCCGTCGCGGGAGAGCGTC
CTGCTCACCTACGGCAGCCGCCCCACCGCCGCCGACCGGGTCCGCCGTCCCGTCACGGTG
ATCGGGGACGGCGCGTTCGCCATGACGCTGGTGCGCGGCGGCCGGCCGCTGCTCCGGGCG
CACCGCATGGAGGCGGACAGCTCCTTCCACGACCTGTTCAAGGTCGAGTACAAGAACAGC
CCCTGGTCGCAGTGGCGCGAGGTGTGCGACGACAACGACCGCTACTCCTTCGAACTGGCC
ATGCACAGCCGCACCAGGCTGAGCAAGCTCGTCGACCAGGTACTCGCGGACGCGGGCATC
GGCAAGGACGACGTGGCCGCCACCCTGATGCAGAACGTCACCTCCAGCGCCTTCGACTTC
TACAAGGCCACGCTGGGGCTGCCGATCCACCCGGTCTGCGCCACCCACCTCGCGGAGCTC
GGCCACCTCGGCCCGATGGACGTGGTCCTGAACCTGGACCGCCTCCTCGCCACCGGTGAG
GTCGGACCCGGTGGACACGTCCTCGTCCTGAGCAACAGCCCGGTCGCCGCGTGGACGGTG
ACCCTGTGGGAGGTGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR013747 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C-terminal (Domain)
[235-323] 4.80000000000001e-08 PF08541
PF08541   ACP_syn_III_C
IPR016038 Thiolase-like, subgroup (Domain)
[6-164] 8.80000000000003e-15 G3DSA:3.40.47.10 [165-324] 2.1e-11 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[12-325] 1.99999636034521e-19 SSF53901
SSF53901   Thiolase-like
SignalP
[1-25] 0.053 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Oxzol_00080 Oxzol_00080   Oxzol_00100 Oxzol_00100
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