Pact_00120 Pact_00120   Pact_00140 Pact_00140

Pact_00130 : CDS information

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Organism
StrainATCC 27456 (=NBRC 13433)
Entry namePactamycin
Contig
Start / Stop / Direction16,396 / 17,352 / + [in whole cluster]
16,396 / 17,352 / + [in contig]
Location16396..17352 [in whole cluster]
16396..17352 [in contig]
TypeCDS
Length957 bp (318 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
ProductN-glycosyltransferase
Product (GenBank)glycosyltransferase
GenepctL
ptmJ
Gene (GenBank)pctL
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[17827660] Cloning of the pactamycin biosynthetic gene cluster and characterization of a crucial glycosyltransferase prior to a unique cyclopentane ring formation. (J Antibiot (Tokyo). , 2007)
[19670201] Deciphering pactamycin biosynthesis and engineered production of new pactamycin analogues. (Chembiochem. , 2009)
comment
[PMID:17827660]
Pactamycin生合成遺伝子クラスターの報告。

PctL: glycosyltransferase

大腸菌にて発現させたrecombinant PctLタンパクを使って酵素反応解析。PctLはcyclopentane ringを形成するより前にUDP-GlcNAcと3-aminoacetophenone間でN-glycosideの形成を触媒すると示された。


[PMID:19670201]
S.pactum ATCC27456のPactamycin生合成遺伝子クラスターの報告。

PtmJ: glycosyltransferase

ptmJ knockout mutantはpactamycin生産能が欠損したことから、PtmJはpactamycin生合成に関与するとしている。
相同性およびNCBI株でのPctLの機能解析実験より、PtmJはUDP-N-acetyl-alpha-D-glucosamineと3-aminoacetophenoneのカップリングに関与するとしている。

close this sectionSequence

selected fasta
>N-glycosyltransferase [glycosyltransferase]
MEATPARPGGGPGDISVSVVVPTRDRTTRLLLTLAALAHQTLDRDRFEVILVDDAPERGA
VDRVLAAAPGTPPLRHARTGGRGPARARNAGAELARGELLLFLDDDTVATPELLTAHLAA
HRDAPGTVVHGTITDLSAFALTPDPPAPRPALTGARGRSIDARRVARLREDAQLLGPRRS
FIERTAAKVIRDPALAGLRWLACIGTSTSVRRADFERAGGFDEGFGELWGGEDLELGLRL
HAAGARFALLDTVAYHLPTARRDTGELLPRFWRLAAERHGDPRLADVGTFLAGRLSPEEL
AARLGTRTAALSPGRAAP
selected fasta
>N-glycosyltransferase [glycosyltransferase]
ATGGAAGCGACGCCCGCCCGGCCGGGGGGAGGTCCCGGCGACATCTCGGTGAGCGTGGTC
GTACCGACCCGGGACCGGACCACGCGTCTGCTGCTCACCCTGGCGGCGCTGGCCCACCAG
ACCCTGGACCGCGACCGGTTCGAGGTCATCCTGGTCGACGACGCGCCCGAACGCGGGGCG
GTGGACCGGGTGCTGGCCGCGGCCCCCGGGACACCGCCGCTGCGGCACGCCCGCACCGGC
GGCCGGGGGCCCGCCCGCGCCCGGAACGCCGGCGCGGAGCTGGCCCGCGGGGAGCTGCTG
CTCTTCCTCGACGACGACACCGTGGCCACCCCCGAACTGCTCACCGCGCACCTGGCGGCG
CACCGCGACGCCCCGGGCACCGTGGTGCACGGCACCATCACCGATCTGTCGGCGTTCGCG
CTGACCCCCGATCCGCCGGCCCCGCGGCCGGCGCTGACCGGTGCCCGCGGCCGCAGCATC
GACGCCCGCCGGGTGGCCCGGCTGCGCGAGGACGCCCAGCTGCTCGGGCCGCGCCGGTCG
TTCATCGAGCGGACCGCCGCCAAGGTCATCCGCGACCCGGCGCTGGCCGGCCTGCGCTGG
CTGGCGTGCATCGGCACCAGCACCAGTGTGCGGCGGGCCGACTTCGAGCGGGCCGGCGGC
TTCGACGAGGGCTTCGGGGAGCTGTGGGGCGGCGAGGACCTGGAGCTGGGGCTGCGGCTG
CACGCCGCCGGGGCCCGCTTCGCGCTGCTGGACACGGTCGCGTACCACCTGCCCACCGCC
CGCCGGGACACCGGCGAACTGCTGCCCCGGTTCTGGCGCCTGGCGGCCGAACGGCACGGC
GATCCGCGGCTGGCCGACGTCGGCACCTTCCTCGCCGGCCGGCTCTCCCCGGAGGAACTG
GCCGCCCGGCTCGGCACCCGCACCGCGGCCCTCTCCCCGGGGAGGGCCGCACCATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0 		No significant hit
SignalP
[1-38] 0.296 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Pact_00120 Pact_00120   Pact_00140 Pact_00140
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