Pact_00200 : CDS information

Location

Organism	Streptomyces pactum
Strain	ATCC 27456 (=NBRC 13433)
Entry name	Pactamycin
Contig	AB303063
Start / Stop / Direction	31,973 / 26,388 / - [in whole cluster] 31,973 / 26,388 / - [in contig]
Location	complement(26388..31973) [in whole cluster] complement(26388..31973) [in contig]
Type	CDS
Length	5,586 bp (1,861 aa)

Annotation

Category	1.1 PKS
Product	polyketide synthase 6-methylsalicylic acid synthase
Product (GenBank)	iterative type I PKS
Gene	pctS ptmQ
Gene (GenBank)	pctS
EC number	2.3.1.165
Keyword	iterative 6-methylsalicylic acid
Note
Note (GenBank)
Reference	ACC A8R0K2 PmId [17827660] Cloning of the pactamycin biosynthetic gene cluster and characterization of a crucial glycosyltransferase prior to a unique cyclopentane ring formation. (J Antibiot (Tokyo). , 2007) [19670201] Deciphering pactamycin biosynthesis and engineered production of new pactamycin analogues. (Chembiochem. , 2009) [21513878] Biosynthetic studies and genetic engineering of pactamycin analogs with improved selectivity toward malarial parasites. (Chem Biol. , 2011) comment [PMID:17827660] Pactamycin生合成遺伝子クラスターの報告。 PctS: iterative type I PKS InterPro: KS-AT-KR-ACP 相同性より機能付けされている。このORFの機能解析はされていない。 PctSは6-methylsalicylic acid (6-MSA)を形成に関与すると推測しており、その生合成経路は2つの仮説を立てている。・PctK(discrete ACP)へacetyl-CoAと共にPctT(ketoacyl-ACP synthase)が運ばれpriming reactionが起こりPctS(iterative type I PKS)により6-MSAを合成。・PctSがacetyl-CoAとself-primingし6-MSAを合成。産生した6-MSAはPctKと一緒にPctTにより活性化し6-MSA-ACPを合成。 [PMID:19670201] S.pactum ATCC27456のPactamycin生合成遺伝子クラスターの報告。 PtmQ: PKS(6-methylsalicylic acid synthase) ポリケタイド合成酵素遺伝子(ptmQ)はhost S.lividansにおいて6-methylsalicylic acid (6-MSA) synthesisをsupportした。 S. pactumでのptmQの不活性株はpactamycinとpactamycate生産能が欠損するが、2つの新しいpactamycin analogues(de-6-MSA-pactamycin and de-6-MSA-pactamycate)が得られたことから6-MSAの合成に関与すると示唆された。また、NBRC株にて6-MSA合成に関与するとされたpctK,T,Mに相当するptmI,R,Kは6-MSAの合成ではなく3-aminoacetophenoneの側鎖形成に関与するのではないかとSchemeで示している。これに関して本文中記述なし。 [PMID:21513878] putative tailoring genesの解析報告(S.pactum ATCC27456)。 ptmHとptmQのdouble knockout mutantでは新規化合物de-6MSA-7-demethyl-7-deoxypactamycinが検出したことから、6-MSAの付着より前にptmHの反応が起こることを示した。
Related Reference	ACC Q0R4P8 NITE Chth_00060 PmId [16793515] Genetic characterization of the chlorothricin gene cluster as a model for spirotetronate antibiotic biosynthesis. (Chem Biol. , 2006) [16677607] Cloning and characterization of a bacterial iterative type I polyketide synthase gene encoding the 6-methylsalicyclic acid synthase. (Biochem Biophys Res Commun. , 2006) [20534347] Insights into bacterial 6-methylsalicylic acid synthase and its engineering to orsellinic acid synthase for spirotetronate generation. (Chem Biol. , 2010) [26833898] Insights into 6-Methylsalicylic Acid Bio-assembly by Using Chemical Probes. (Angew Chem Int Ed Engl. , 2016) comment BLAST id54% Streptomyces antibioticus ChlB1 --- [PMID: 16793515] chlorothricin(CHL) gene clusterに関する文献。 chlB1(1,756 aa): Type I PKS: KS-AT-KR-DH-ACP chlB1 にはtype I PKS domainが存在することが示されており、chlB1の不活化変異株を作成し、その生産物をLC-MS, HR-MSを使って、CHLではなく、methylsalicylic acid moietyが欠損しているdesmethylsalicyl CHL(DM-CHL)であることを確認している。また、このchlB1不活化変異株にchlB1遺伝子を相補すると、DM-CHLはCHLへと変換されることをHPLCで精製してLC-MSを使うことで確認している。 --- [PMID: 16677607] 6-methylsalicylic acid synthaseとして機能するiterative type I PKS gene chlB1のクローニング、配列解析、特徴づけ。 --- [PMID:20534347] ChlB1をS. albusで発現し、6-methylsalicylic acid(6-MSA)が発現されていることをHPLC-MSで確認。 site-specific mutagenesisによるChlB1のDH and KR domainsの機能的解析。 DH domain不活化株TL1072(H947A)は6-MSA非産生、(H947F)は少ないけど6-MSA産生あり。 ChlB1-DH domainのdehydration活性は重要だが、6-MSAを産生するのに不可欠でないかもしれない。 KR domain不活化株TL1076 (Y1540F) は、6-MSAのC-2が非還元のorsellinic acid蓄積。 --- [PMID: 26833898](2016) 6-methylsalicylic acid synthase による6-MSA生合成の中間体検出。 TH domainを介する経路の裏付けとなっている。
	ACC P87162 PmId [20304931] ( , ) comment BLAST id37% Aspergillus terreus_atX 6-methylsalicylic acid synthase 6-methylsalicylic acid synthase_ATXのdehydratase(DH) domainの機能的検証。 DH domainはACPに繋がれたbeta-hydroxytriketide中間体のdehydrationに関連しないが、ACPから6- methylsalicylic acidを放出するためにthioester hydrolysisを触媒することが明らかになった。よってDH domainからTH domain(Gly904 - Thr1048の145aa)へ改名。構造解析報告のあるEryDH4とのalignmentから、H972XXXGXXXXP motif and Asp1129 が触媒に重要な残基であると提唱されている。著者らの既報で、ATX H972A mutantがbeta-hydroxy triketideのような産物をもたらさないことが確認されている。
	ACC P22367 PmId [26833898] Insights into 6-Methylsalicylic Acid Bio-assembly by Using Chemical Probes. (Angew Chem Int Ed Engl. , 2016) comment BLAST id38% Penicillium patulum (Penicillium griseofulvum) 6-methylsalicylic acid synthase (EC:2.3.1.165)

PKS/NRPS Module

acetyl-CoA
malonyl-CoA

KS	62..436

AT	642..957

TH	965..1129

KR	1502..1695

ACP	1779..1856

Sequence

>polyketide synthase [iterative type I PKS]
MSAAKDGHRSEADGSRQVPEGTAGAGQVTYGTDGTDRAAGTRQVTHGTDGAAGTRPAAHG
ADEPVAIIGMSCRFPGGADSPDAFWELLAQGRDGIRDGSARWAAYAAAGHEHAAVVRRTT
GFGGFLDDIAGFDAEFFGISPREAELMDPQQRLLLELAWEALEHAGLPPLELAGGDCGVF
VGVGSDDYGRRLLEDLPRIEPWTGIGASMCATANRVSHSLDLRGPSLAVDTACSASLVAV
HLACRSLLAGESEVALAAGVNLMVAPGLSVTLDRAGATSPDGRSKPFDAAADGYGRGEGA
GVVVLKRLADAERAGDPVLAVIRGTGVSQDGRTDGIMAPSGEAQADLLRRTYRRCGIAPG
TVDYVEAHGTGTVAGDPLEAGALGAVFGAGRPADRPCLIGSVKGNIGHLEAGSGIAGVIK
TVLALGREEIPPSVHFSAPNPRIPWETARLRVATGRTPWPRGDGPRRAGVSSFGYGGTIA
HVVLEEAPAPAPGRAPAPEPAVGAEGAVGTERAVVTEPAPAAGPAPAAGPAAASGPAAAP
EAAGAEAGPPSLLFPLSARSREAVRADAARLADWLDGPGAGAAPASLAHTLGVRRSHLEH
RVAVVARDRAELAARLRHVAAGEAAPGVTEGTVVEGAGTGVVWVFSGTGAQWPGMGRELL
ATEPAFAAVIDRIDPVYAAEIGTTARRMIQEGDVSRVDVAQAMIFAVQAGLTAVWTSLGV
RPAAVVGHSLGEIAAAVAAGVLSVEDGARLVCRRSVLLRRVAGAGGMLLVGLSAEEATDR
LGTADDVVPAVLASPTSTVLSGPVARIDALAREWSADPELLVRRVDSEVAFHSPQMDPLL
DELARAAAPLTVHPPAVPIYGTALADPRDPAPRGGAYWAANLRNPVRLAGAVAAAAEDGF
RAFLEISAHPVVGHSVQETLDAAGAAGHCVAGSLRRDAGGRDQLLLNAGLLYCHGAAPDR
AAFPDGELLALPPRTWRRRTYWRDLPARREDRGRHDPAGRTLLGPRTVLAGATPLHLWRT
RVDMETRPYPGHHTIQGTEIVPAAVVLQTFLDATGTGPGPRGLTGVDFALPLTLEPARDI
DVTAQDGVVRLLSRPAATGTDSDTGRGPEGGGSDAGPDGGEREWLTHASASAAEDLTPPD
AAPPAGGPGTVLPPDRAHADLAAVGVPTMAFPWEVTRLERLPDGLRAEVTAADGPEGTPD
GWAPLLDAALSVAAVAFPGTPALRVVAGVSRVWTAGGAPDRARIEARVTGPVTEAAGTVD
VTLVAADGRTVAVLAGVRYAGAAADQPRAAEPEELLYATEWHPLTVDPADLPLPPRPLVL
VGPAEGPGPALRARCTETGRRVALLADPDGLDPLLDRAGGPVDVLVLPVAAEPAEPAADR
AVREAWLLARTARRLAARPPGQARLWSLTVGVREAAGADSVAQAARWGLGRIIGGEHPDL
WGGTLDLAPDHTAADLATALDVSAAGPGEDVVAVRGGRAEANRLVRCAAPPARPPLRCRA
DGSYLITGGLGGLGGEIARRLVELGARRLVLAGRSALPPRSAWDTVTDPEQARRIATVRR
LEALGATVRVVALDIADAGAAAAALDPDALDLPPIRGVVHAAGVTDDRLVEQLDRDALAA
VIRPKAAGAFTLHRLFPPGSLDFVVHFSSCGQLLGLTGQGAYAAANAFLDAVAGYERAAG
SAGSMSLAWTSWRGIGLADNAAVDAELAAHGVGDVTVPEALAAWDHAARLGLPSLAVLRT
VPLPEGTRRTGLLRDVTDPEPATPAPGGAAAGAGIDGLSGEELRAALRERTAALIVGEMR
WDPAGLDPDRSLLKMGMDSVMAIVIRRKLEQLLGRKLPANLVWHQQTVSNIVDYLVTTSR
P

>polyketide synthase [iterative type I PKS]
GTGAGCGCGGCCAAGGACGGGCACCGGTCGGAGGCGGACGGCAGCCGGCAGGTGCCGGAG
GGTACGGCCGGCGCCGGGCAGGTCACGTACGGCACGGACGGTACGGACAGGGCGGCCGGC
ACCCGGCAGGTCACGCACGGCACGGACGGCGCGGCCGGCACCCGGCCGGCAGCGCACGGC
GCCGACGAGCCCGTCGCGATCATCGGGATGAGCTGCCGGTTCCCCGGCGGCGCGGACTCC
CCCGACGCCTTCTGGGAGCTGCTGGCGCAGGGCCGGGACGGCATCCGGGACGGTTCCGCG
CGGTGGGCGGCGTACGCGGCGGCGGGACACGAGCACGCCGCGGTGGTGCGCCGGACCACC
GGGTTCGGCGGCTTCCTCGACGACATCGCCGGGTTCGACGCCGAGTTCTTCGGCATCTCG
CCGCGCGAGGCGGAGCTGATGGACCCGCAGCAGCGGCTGCTGCTGGAGCTGGCCTGGGAG
GCGCTGGAGCACGCGGGGCTGCCGCCGCTGGAGCTGGCCGGCGGCGACTGCGGGGTCTTC
GTGGGCGTCGGGTCGGACGACTACGGGCGCCGGCTGCTGGAGGACCTGCCCCGGATCGAG
CCGTGGACCGGCATCGGCGCCTCGATGTGCGCCACCGCGAACCGCGTCTCGCACAGCCTG
GACCTGCGCGGTCCCAGCCTGGCGGTGGACACCGCCTGCTCGGCGTCGCTGGTCGCCGTG
CACCTGGCGTGCCGGAGCCTGCTGGCCGGGGAGTCGGAGGTGGCGCTGGCCGCGGGGGTC
AACCTCATGGTGGCCCCCGGGCTGTCGGTGACCCTGGACCGGGCGGGCGCCACCTCCCCG
GACGGCCGCAGCAAGCCGTTCGACGCGGCGGCCGACGGCTACGGCCGCGGTGAGGGCGCG
GGCGTGGTGGTGCTGAAGCGGCTGGCGGACGCGGAGCGGGCGGGTGACCCGGTGCTGGCC
GTCATCCGCGGCACCGGGGTGAGCCAGGACGGCCGTACCGACGGGATCATGGCGCCCAGC
GGCGAGGCCCAGGCGGACCTGCTGCGGCGGACGTACCGGCGGTGCGGCATCGCGCCCGGC
ACGGTCGACTACGTGGAGGCGCACGGGACCGGCACGGTGGCCGGCGACCCGCTGGAGGCG
GGGGCGCTCGGCGCGGTGTTCGGCGCGGGCCGGCCCGCGGACCGGCCCTGTCTGATCGGC
TCGGTGAAGGGCAACATCGGTCACCTGGAGGCGGGTTCCGGGATCGCCGGGGTGATCAAG
ACGGTGCTCGCCCTGGGCCGGGAGGAGATCCCGCCCAGCGTCCACTTCTCCGCCCCCAAC
CCCCGTATCCCGTGGGAGACCGCGCGGCTGCGGGTGGCCACCGGACGCACCCCCTGGCCG
CGCGGCGACGGGCCCCGCCGGGCGGGCGTGTCCAGCTTCGGTTACGGCGGCACCATCGCC
CATGTCGTCCTGGAGGAGGCCCCGGCCCCCGCCCCGGGACGCGCCCCCGCCCCGGAGCCG
GCCGTCGGCGCGGAAGGGGCCGTCGGCACGGAACGGGCCGTCGTCACGGAACCGGCTCCC
GCCGCGGGACCGGCTCCCGCCGCGGGACCGGCCGCGGCCTCCGGACCGGCCGCCGCCCCG
GAGGCCGCGGGCGCCGAGGCCGGGCCACCGTCCCTGCTCTTCCCGCTCTCCGCCCGGTCG
CGGGAGGCGGTGCGGGCGGACGCCGCCCGGCTCGCCGACTGGCTGGACGGGCCCGGCGCC
GGCGCCGCGCCGGCCTCGCTCGCGCACACCCTCGGGGTCCGGCGCAGCCATCTGGAGCAC
CGGGTCGCGGTGGTCGCCCGGGACCGCGCGGAACTCGCCGCCCGGCTGCGCCACGTCGCC
GCCGGCGAGGCCGCGCCCGGGGTCACCGAGGGCACGGTGGTGGAGGGCGCGGGGACCGGC
GTGGTGTGGGTGTTCTCCGGCACCGGTGCCCAGTGGCCCGGCATGGGCCGCGAACTGCTC
GCCACCGAGCCCGCGTTCGCCGCCGTGATCGACCGGATCGACCCGGTCTACGCCGCGGAG
ATCGGCACCACCGCGCGCCGGATGATCCAGGAGGGGGACGTCTCCCGGGTGGACGTGGCC
CAGGCCATGATCTTCGCGGTGCAGGCGGGGCTGACCGCCGTCTGGACGTCGCTCGGCGTC
CGGCCGGCGGCCGTGGTGGGCCACTCGCTGGGCGAGATCGCCGCCGCGGTGGCCGCCGGG
GTGCTCTCCGTGGAGGACGGGGCGCGGCTGGTCTGCCGCCGCAGCGTGCTGCTGCGTCGG
GTGGCCGGCGCGGGCGGCATGCTGCTGGTGGGGCTCTCCGCCGAGGAGGCCACCGACCGT
CTCGGCACGGCGGACGACGTGGTGCCGGCCGTCCTCGCCTCACCCACCAGCACGGTGCTC
TCCGGCCCGGTGGCCCGGATCGACGCCCTGGCCCGCGAGTGGTCGGCGGACCCGGAGCTG
CTGGTGCGGCGGGTGGACAGCGAGGTGGCGTTCCACAGCCCGCAGATGGACCCGCTGCTC
GACGAACTGGCCCGGGCGGCGGCCCCGCTGACCGTCCACCCGCCGGCCGTGCCGATCTAC
GGCACCGCGCTGGCCGACCCGCGCGACCCGGCCCCGCGGGGCGGCGCCTACTGGGCGGCG
AACCTGCGCAACCCGGTCCGGCTGGCCGGCGCGGTCGCCGCGGCGGCCGAGGACGGCTTC
CGCGCCTTCCTGGAGATCTCGGCCCACCCGGTGGTGGGCCACTCGGTGCAGGAGACGCTC
GACGCGGCCGGCGCCGCCGGCCACTGTGTCGCCGGCAGCCTCCGCCGGGACGCCGGGGGC
CGGGACCAGCTGCTGCTCAACGCCGGTCTGCTGTACTGCCACGGCGCCGCGCCGGACCGG
GCCGCGTTCCCGGACGGGGAGCTGCTGGCGCTGCCGCCCCGGACCTGGCGGCGCCGCACG
TACTGGCGCGACCTGCCGGCCCGGCGGGAGGACCGGGGGCGGCACGACCCGGCCGGCCGG
ACCCTGCTGGGGCCGCGCACCGTCCTGGCCGGGGCGACCCCGCTGCACCTGTGGCGGACC
CGGGTGGACATGGAGACCCGGCCCTACCCCGGGCACCACACCATCCAGGGCACCGAGATC
GTGCCGGCGGCGGTGGTGCTCCAGACGTTCCTGGACGCCACCGGAACCGGCCCGGGCCCG
CGGGGCCTGACCGGGGTGGACTTCGCCCTGCCGCTGACCCTGGAGCCGGCCCGGGACATC
GACGTCACCGCGCAGGACGGGGTGGTGCGGCTGCTGTCCCGGCCGGCCGCGACCGGCACC
GACAGCGACACCGGCCGCGGACCGGAGGGCGGCGGGTCGGACGCCGGCCCGGACGGCGGG
GAGCGGGAGTGGCTGACGCACGCCTCGGCGTCGGCGGCCGAGGACCTGACACCGCCGGAC
GCGGCGCCGCCGGCCGGCGGGCCGGGCACGGTTCTGCCGCCCGACCGGGCGCACGCCGAC
CTGGCCGCGGTCGGCGTACCGACCATGGCCTTCCCCTGGGAGGTGACCCGGCTGGAGCGG
CTGCCGGACGGGCTGCGCGCCGAGGTGACCGCCGCCGACGGGCCGGAGGGGACACCGGAC
GGCTGGGCCCCGCTGCTGGACGCCGCGCTCTCCGTCGCCGCGGTCGCCTTCCCCGGCACC
CCGGCGCTGCGGGTCGTGGCCGGGGTGTCCCGGGTGTGGACGGCGGGCGGTGCGCCGGAC
CGCGCCCGGATCGAGGCGCGGGTGACCGGCCCGGTCACCGAGGCCGCCGGCACGGTGGAC
GTGACCCTGGTGGCCGCGGACGGGCGGACCGTCGCGGTGCTGGCCGGGGTCCGGTACGCG
GGGGCGGCGGCAGACCAGCCCCGGGCCGCCGAGCCGGAGGAGCTGCTGTACGCGACCGAG
TGGCACCCGCTCACCGTGGACCCCGCCGACCTGCCGCTGCCGCCCCGGCCGCTGGTGCTG
GTCGGCCCCGCCGAGGGGCCGGGCCCGGCGCTGCGGGCGCGGTGCACGGAGACCGGCCGG
CGGGTGGCGCTACTGGCCGACCCGGACGGGCTGGACCCGCTGCTCGACCGGGCCGGCGGC
CCGGTGGACGTGCTGGTGCTGCCCGTCGCGGCGGAACCGGCGGAACCGGCCGCGGACCGC
GCGGTACGGGAGGCGTGGCTGCTGGCCCGCACCGCGCGGCGGCTCGCCGCCCGGCCGCCC
GGACAGGCCCGGCTGTGGTCGCTGACCGTGGGCGTCCGGGAGGCGGCCGGCGCCGACTCG
GTGGCCCAGGCGGCCCGCTGGGGGCTGGGCCGGATCATCGGCGGGGAGCACCCCGACCTG
TGGGGCGGCACCCTCGACCTGGCCCCCGACCACACCGCCGCGGACCTGGCCACCGCGCTG
GACGTGTCGGCCGCGGGCCCCGGCGAGGACGTGGTGGCGGTCCGCGGCGGCCGGGCCGAG
GCCAACCGGCTGGTGCGCTGCGCCGCGCCGCCGGCCCGCCCGCCGCTGCGGTGCCGGGCC
GACGGCAGCTACCTGATCACCGGTGGACTGGGCGGACTGGGCGGCGAGATCGCCCGCCGG
CTGGTGGAGCTGGGCGCCCGCCGGCTGGTGCTCGCCGGGCGCTCCGCGCTGCCGCCGCGC
TCGGCGTGGGACACGGTCACCGATCCGGAACAGGCCCGCCGGATCGCGACCGTCCGCCGG
CTGGAGGCGCTCGGCGCCACGGTGCGGGTGGTCGCCCTGGACATCGCCGACGCCGGGGCG
GCGGCCGCCGCCCTGGACCCGGACGCCCTGGACCTGCCGCCGATCCGCGGCGTGGTGCAC
GCGGCCGGCGTGACCGACGACCGGCTGGTGGAGCAGCTCGACCGGGACGCCCTCGCCGCG
GTGATCCGCCCCAAGGCCGCCGGGGCGTTCACCCTGCACCGGCTCTTCCCGCCCGGCAGC
CTCGACTTCGTGGTGCACTTCTCGTCCTGCGGCCAGCTGCTCGGACTCACCGGGCAGGGC
GCCTACGCGGCGGCCAACGCGTTCCTGGACGCGGTGGCCGGATACGAGCGGGCCGCCGGG
TCGGCCGGCAGCATGAGCCTGGCGTGGACCTCGTGGCGCGGGATCGGCCTCGCCGACAAC
GCGGCGGTGGACGCCGAACTCGCCGCCCACGGGGTCGGGGACGTCACGGTGCCGGAGGCG
CTGGCCGCCTGGGACCACGCCGCCCGGCTCGGCCTGCCCTCGCTGGCGGTCCTGCGGACC
GTGCCGCTGCCGGAGGGCACCCGCCGCACCGGCCTGCTGCGGGACGTCACCGACCCCGAG
CCGGCCACCCCGGCACCCGGCGGGGCGGCGGCGGGCGCGGGGATCGACGGGCTGTCCGGG
GAGGAGCTCCGGGCAGCGCTGCGGGAGCGGACCGCCGCCCTGATCGTCGGGGAGATGCGG
TGGGACCCGGCCGGACTGGACCCGGACCGGTCGCTGCTGAAGATGGGCATGGACTCGGTC
ATGGCGATCGTCATCCGGCGGAAGCTGGAGCAACTGCTGGGCCGCAAACTCCCGGCCAAC
CTCGTCTGGCACCAGCAGACGGTGTCCAACATCGTGGACTACCTGGTCACCACGTCCCGC
CCCTGA

[1] KS	62..436

[1] AT	642..957

[1] acetyl-CoA malonyl-CoA	829..833

[1] TH	965..1129

[1] KR	1502..1695

[1] ACP	1779..1856

[1] KS	184..1308

[1] AT	1924..2871

[1] acetyl-CoA malonyl-CoA	2485..2499

[1] TH	2893..3387

[1] KR	4504..5085

[1] ACP	5335..5568

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001227 Acyl transferase domain (Domain) G3DSA:3.40.366.10 Ac_transferase_reg IPR009081 Acyl carrier protein-like (Domain) SSF47336 ACP_like PF00550 PP-binding PS50075 ACP_DOMAIN G3DSA:1.10.1200.10 ACP_like IPR013968 Polyketide synthase, KR (Domain) PF08659 KR IPR014030 Beta-ketoacyl synthase, N-terminal (Domain) PF00109 ketoacyl-synt IPR014031 Beta-ketoacyl synthase, C-terminal (Domain) PF02801 Ketoacyl-synt_C IPR014043 Acyl transferase (Domain) PF00698 Acyl_transf_1 IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain) SSF52151 Acyl_Trfase/lysoPlipase IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain) SSF55048 Malonyl_transacylase_ACP-bd IPR016038 Thiolase-like, subgroup (Domain) G3DSA:3.40.47.10 Thiolase-like_subgr IPR016039 Thiolase-like (Domain) SSF53901 Thiolase-like IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd IPR018201 Beta-ketoacyl synthase, active site (Active_site) PS00606 B_KETOACYL_SYNTHASE IPR020801 Polyketide synthase, acyl transferase domain (Domain) SM00827 PKS_AT IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain) SM00825 PKS_KS IPR020842 Polyketide synthase/Fatty acid synthase, KR (Domain) SM00822 PKS_KR
SignalP	No significant hit
TMHMM	No significant hit

Pact_00190 Pact_00210