Polk_00350 : CDS information

Location

Organism	Streptomyces diastatochromogenes
Strain	Tü6028
Entry name	Polyketomycin
Contig	FJ483966
Start / Stop / Direction	43,560 / 42,073 / - [in whole cluster] 43,560 / 42,073 / - [in contig]
Location	complement(42073..43560) [in whole cluster] complement(42073..43560) [in contig]
Type	CDS
Length	1,488 bp (495 aa)

Annotation

Category	5.1 general function
Product	putative ligase
Product (GenBank)	PokL
Gene
Gene (GenBank)	pokL
EC number
Keyword
Note
Note (GenBank)	putative acyl-CoA ligase
Reference	ACC C0JWD0 PmId [19266534] Organisation of the biosynthetic gene cluster and tailoring enzymes in the biosynthesis of the tetracyclic quinone glycoside antibiotic polyketomycin. (Chembiochem. , 2009) comment polyketomycin生合成clusterの解析論文。機能推定は配列解析から。 pokL: acyl-CoA ligase PokM3(putative AMP ligase) or PokL(putative acyl-CoA ligase)によって、3,6-dimethyl-salicylic acidが糖に付着されるかもしれない。
Related Reference	ACC P41636 PmId [9008388] Molecular cloning of 4-coumarate:coenzyme A ligase in loblolly pine and the roles of this enzyme in the biosynthesis of lignin in compression wood. (Plant Physiol. , 1997) comment Blast 54th, id32%, 1e-53 Pinus taeda_4CL 4-coumarate--CoA ligase(EC 6.2.1.12) 圧縮ストレスにより木部での発現変化する 4CL activity with 4-coumaric acid: 上昇 with ferulic acid: 変化なし with caffeic acid: 低下

Sequence

>putative ligase [PokL]
MREPVERLDELPRLAAEAHPDRVALRTDRSDALTFGELEQRVSGCAARIRALVGGPGTTV
AITSVLHPDFAVAYYGTLRAGHVVVPVNPFLRAKGLEALVAASGARLVLTTEEIAAELGD
TVPSLDIGDRTLWDTGPLREAPAVRPDDVASIHFTSGTTGAPKGVRQTHHNLAVNAAQTV
HAHGLDDSSVTVNHLPTFHPMHLNSALFAAAEQVLATDPDLAASLRTANEARATRYYSLP
MRLSRLAARPDLSELRLDTVEAVFSGGSALAVPAARRLGDHFGIPVLQGYGLAETAPMTH
CGSRTRPKEGSCGPPVPRTECRIVDLATGKALGPGERGEIQVRGPQLMKGYLGEPDGGAL
DAEGWFATGDVGEVDEDDCLFVVDRLKDVFKCDNWLVSPQEIEQVLLAHPAVADCAVVDH
PDEFSGAVAVALVVTRRPEEPEALDGIAADVNSGLPYYQHIRHIRAVPDIARSGGGKVRR
QDLRRLFTELFATRG

>putative ligase [PokL]
GTGCGTGAGCCCGTCGAGCGCCTGGACGAGCTGCCGCGGCTCGCCGCCGAGGCCCACCCC
GACCGTGTCGCCCTGCGCACGGACCGCAGCGACGCGCTGACCTTCGGGGAGCTGGAGCAG
CGGGTGAGCGGCTGCGCGGCACGCATCCGCGCTCTGGTGGGCGGGCCCGGCACGACCGTC
GCGATCACCTCGGTCCTCCATCCCGACTTCGCCGTGGCGTACTACGGCACGCTGCGCGCG
GGCCATGTCGTCGTGCCGGTCAATCCCTTCCTCCGGGCGAAGGGTCTGGAGGCCCTCGTC
GCCGCGAGCGGCGCACGGCTGGTGCTCACGACGGAGGAGATCGCCGCCGAGCTCGGCGAC
ACGGTGCCGTCGCTGGACATCGGCGACCGGACGCTGTGGGACACCGGACCGCTGCGGGAG
GCGCCCGCCGTGCGCCCCGACGACGTCGCGTCCATCCACTTCACCAGCGGCACGACCGGC
GCCCCGAAGGGCGTGCGGCAGACCCATCACAACCTGGCGGTCAACGCGGCGCAGACCGTG
CACGCGCACGGTCTGGACGACAGCTCGGTGACCGTCAACCATCTGCCCACGTTCCACCCG
ATGCACCTGAACTCGGCGCTGTTCGCGGCCGCCGAGCAGGTGCTGGCCACCGATCCGGAC
CTGGCGGCGTCGCTGCGGACGGCGAACGAGGCGCGGGCCACCCGGTACTACAGCCTGCCG
ATGCGGCTCAGCCGTCTCGCGGCGCGACCGGACCTGTCGGAGCTGCGGCTCGACACCGTG
GAGGCCGTGTTCTCCGGCGGCTCCGCACTGGCGGTCCCGGCGGCGCGCAGGCTCGGCGAC
CACTTCGGGATCCCGGTCCTCCAGGGGTACGGGCTCGCCGAGACGGCGCCGATGACCCAC
TGCGGCAGCCGCACCCGGCCCAAGGAGGGTTCGTGCGGACCCCCGGTCCCCCGCACCGAG
TGCCGGATCGTCGACCTCGCCACCGGCAAGGCACTCGGCCCCGGTGAGCGCGGCGAGATC
CAGGTGCGCGGCCCGCAGTTGATGAAGGGCTACCTGGGCGAGCCCGACGGCGGCGCCCTG
GACGCCGAGGGCTGGTTCGCCACCGGGGACGTCGGCGAGGTCGACGAGGACGACTGCCTC
TTCGTCGTCGACCGGCTCAAGGACGTCTTCAAGTGCGACAACTGGCTGGTCTCCCCGCAG
GAGATCGAGCAGGTGCTGCTCGCGCACCCCGCCGTGGCGGACTGCGCGGTCGTCGACCAC
CCGGACGAGTTCAGCGGCGCCGTGGCGGTCGCCCTCGTGGTGACCCGTCGTCCCGAGGAG
CCCGAAGCACTGGACGGCATCGCCGCGGACGTCAACTCCGGCCTGCCGTACTACCAGCAC
ATCCGGCACATCCGGGCGGTCCCCGACATCGCCCGGTCCGGCGGCGGCAAGGTCCGCCGC
CAGGACCTGCGCCGGCTCTTCACGGAACTGTTCGCCACGCGCGGATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000873 AMP-dependent synthetase/ligase (Domain) PF00501 AMP-binding IPR020845 AMP-binding, conserved site (Conserved_site) PS00455 AMP_BINDING
SignalP	No significant hit
TMHMM	No significant hit

Polk_00340 Polk_00360