Pyrlo_00050 : CDS information

Location

Organism	Actinosporangium vitaminophilum
Strain	ATCC 31673 (=NBRC 14294)
Entry name	Pyrrolomycin
Contig	EF140901
Start / Stop / Direction	5,513 / 6,580 / + [in whole cluster] 5,513 / 6,580 / + [in contig]
Location	5513..6580 [in whole cluster] 5513..6580 [in contig]
Type	CDS
Length	1,068 bp (355 aa)

Annotation

Category	5.1 general function
Product	putative 2-oxoglutarate ferredoxin oxidoreductase beta subunit
Product (GenBank)	2-oxoglutarate ferredoxin oxidoreductase beta subunit
Gene
Gene (GenBank)	pyr5
EC number	1.2.7.3
Keyword
Note
Note (GenBank)
Reference	ACC A3R4P6 PmId [17158935] Cloning and characterization of the pyrrolomycin biosynthetic gene clusters from Actinosporangium vitaminophilum ATCC 31673 and Streptomyces sp. strain UC 11065. (Antimicrob Agents Chemother. , 2007) comment pyrrolomycin biosynthetic gene clustersに関する文献。 pyr5(355 a.a.): 2-oxoglutarate ferredoxin oxidoreductase beta subunit pyr5自体の機能実験は行われていないが、相同性より2-oxoglutarate ferredoxin oxidoreductase beta subunitと予測している。同じpyr gene cluster中に存在するpyr18, pyr19によってエンコードされるnitrate reductaseに対して、還元されたferredoxinを供給する役割を持つと推測している。
Related Reference	ACC Q9AJL9 PmId [11401501] The genes for anabolic 2-oxoglutarate: ferredoxin oxidoreductase from Hydrogenobacter thermophilus TK-6. (Biochem Biophys Res Commun. , 2001) [16978355] Role of two 2-oxoglutarate:ferredoxin oxidoreductases in Hydrogenobacter thermophilus under aerobic and anaerobic conditions. (FEMS Microbiol Lett. , 2006) comment <2-oxoglutarate ferredoxin oxidoreductase beta subunit(korB), Hydrogenobacter thermophilus, BLAST 176; id=47%> Hydrogenobacter thermophilusにおいて、2-Oxoglutarate: ferredoxin oxidoreductase (OGOR)の遺伝子korABのクローニングを行い、大腸菌で発現させて活性を確認しており、2-oxoglutarateに対する高い基質特異性を示すことが述べられている。また、Korは嫌気条件下の生育に必須であることを示している。

Sequence

>putative 2-oxoglutarate ferredoxin oxidoreductase beta subunit [2-oxoglutarate ferredoxin oxidoreductase beta subunit]
MRHPLALVPTTDTKQTARDFKSDQEVRWCPGCGDYAILAAVQGFLPDLGIARENIVFISG
IGCSSRFPYYMNTYGMHSIHGRAPAIATGLSTTRPDLSVWVITGDGDALSIGGNHLIHAL
RRNVNLNILLFNNRIYGLTKGQYSPTSETGKITKSTPMGSLDTPFNPISLALGAEASFVA
RTIDSDRTHLTDTLRQAAHHPGTSLVEIYQNCNIFNDGAFEPLKNKDTQHHHLIRLTHGQ
PIRFGPPAPDGHGSKALTRNPTTGTLEITDLTQTPNPPNILIHDAHNPDPTTAYALSRLA
DPHTLTHTPIGVFRDITRPVYDHTMTHQLDHATEHQGKGDLAALLAGNDTWTVTR

>putative 2-oxoglutarate ferredoxin oxidoreductase beta subunit [2-oxoglutarate ferredoxin oxidoreductase beta subunit]
GTGCGTCACCCGCTGGCGCTGGTGCCCACCACCGACACCAAGCAAACCGCCCGGGACTTC
AAGTCCGACCAGGAAGTGCGCTGGTGCCCCGGCTGCGGCGACTACGCCATCCTCGCCGCC
GTCCAAGGCTTCCTACCCGACCTGGGCATCGCCCGCGAGAACATCGTGTTCATCTCCGGG
ATCGGCTGCTCCTCCCGATTCCCCTACTACATGAACACCTACGGGATGCACTCCATCCAC
GGCCGCGCCCCCGCCATCGCCACCGGACTGTCCACCACCCGCCCCGACCTGTCCGTCTGG
GTCATCACCGGCGACGGCGACGCCCTGTCCATCGGCGGCAACCACCTCATCCACGCCCTA
CGCCGCAACGTCAACCTCAACATCCTGCTGTTCAACAACCGCATCTACGGCCTCACCAAA
GGCCAGTACTCCCCCACCTCAGAAACCGGCAAAATCACCAAATCCACCCCCATGGGAAGC
CTCGACACCCCCTTCAACCCCATCTCCCTCGCCCTGGGAGCCGAAGCCTCCTTCGTCGCC
CGCACCATCGACTCCGACCGCACCCACCTCACCGACACCCTCCGCCAAGCCGCCCACCAC
CCCGGCACCTCCCTCGTCGAGATCTACCAAAACTGCAACATCTTCAACGACGGCGCCTTC
GAACCCCTGAAGAACAAAGACACCCAACACCACCACCTCATCCGCCTCACCCACGGCCAA
CCCATCCGCTTCGGCCCCCCCGCCCCCGACGGACACGGCAGCAAAGCCCTCACCCGCAAC
CCCACCACCGGCACCCTCGAAATCACCGACCTCACCCAAACCCCCAACCCCCCCAACATC
CTCATCCACGACGCCCACAACCCCGACCCCACCACCGCCTACGCCCTCTCCCGCCTCGCC
GACCCCCACACCCTCACCCACACCCCCATCGGCGTCTTCCGCGACATCACCCGCCCCGTC
TACGACCACACCATGACCCACCAACTCGACCACGCCACCGAACACCAGGGCAAAGGCGAC
CTCGCCGCCCTCCTGGCAGGAAACGACACCTGGACCGTCACCCGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR011766 Thiamine pyrophosphate enzyme, C-terminal TPP-binding (Domain) PF02775 TPP_enzyme_C
SignalP	No significant hit
TMHMM	No significant hit

Pyrlo_00040 Pyrlo_00060