Pyrlo_00060 : CDS information

Location

Organism	Actinosporangium vitaminophilum
Strain	ATCC 31673 (=NBRC 14294)
Entry name	Pyrrolomycin
Contig	EF140901
Start / Stop / Direction	6,626 / 8,065 / + [in whole cluster] 6,626 / 8,065 / + [in contig]
Location	6626..8065 [in whole cluster] 6626..8065 [in contig]
Type	CDS
Length	1,440 bp (479 aa)

Annotation

Category	4.3 transport
Product	putative transporter
Product (GenBank)	membrane antiporter
Gene
Gene (GenBank)	pyr6
EC number
Keyword
Note
Note (GenBank)
Reference	ACC A3R4P7 PmId [17158935] Cloning and characterization of the pyrrolomycin biosynthetic gene clusters from Actinosporangium vitaminophilum ATCC 31673 and Streptomyces sp. strain UC 11065. (Antimicrob Agents Chemother. , 2007) comment pyrrolomycin biosynthetic gene clustersに関する文献。 pyr6(479 a.a.): membrane antiporter pyr6自体の機能実験は行われていないが、membrane cation antiporter proteins GntQ, StaNとの相同性が示されている。また、pyrrolomycinに対する自己耐性への可能性が記載されている。
Family/Domain	FD IPR006153 comment [IPR006153] Cation/H+ exchanger (Domain) Function GO:0015299 solute:proton antiporter activity

Sequence

>putative transporter [membrane antiporter]
MSSNHEHAEWIVTVAIGDVALVLAVGTLFVLLARRIKQPAVIGEITAGICLGPSLLGLLP
GDLPEQLFPSDVRPYLNVVAQIGLLLFMFIIGWEFDYASMRGRRKKLGTIWICAAALPLS
MGMGLAVLLHGSNDVVNGKHIRMVDFALYIGIAMSIAAFPVLARIITDHRLQFTRVGTLA
LALAALDDILAWSMLAVVVALVTASGTGGFLSVIGWGAVYIVVMLVIVRPALAYLARKIT
SASAPYLAVAAAAGTLCSAYITSEIGLHAIFGAFVFGLVMPRGPQPELMHRAAMVPLENV
SKLLLPLFFVVTGLSVDLTAMTSDGLIQMTLIICVAITGKLGGVLLSARVTGMSWQDSTI
LGLLMNTRGLTELVILNVGLSLGLLSVELFTAMVMMALVTTGMTSPILSMMLRRAAPHSE
AAVPAHLKDGGDESDASAAAVPSAANGSGPVAGVENGASPVHRDEAGRNSYLPTPQGGD

>putative transporter [membrane antiporter]
GTGAGCAGTAACCACGAACACGCGGAGTGGATCGTCACCGTCGCCATCGGTGACGTGGCG
TTGGTCCTCGCCGTCGGAACGCTATTCGTGCTGCTGGCGCGCCGCATCAAGCAGCCCGCG
GTCATCGGCGAGATCACCGCCGGCATCTGCCTGGGGCCCAGCCTGCTCGGACTCCTTCCG
GGTGACCTCCCCGAGCAGCTCTTCCCCAGCGACGTCAGGCCCTACCTCAACGTCGTCGCC
CAGATCGGCCTCCTGCTGTTCATGTTCATCATCGGCTGGGAGTTCGACTACGCCTCGATG
CGCGGGCGCCGCAAGAAACTGGGGACCATCTGGATCTGCGCCGCCGCCCTGCCCCTGTCC
ATGGGCATGGGGCTCGCCGTGCTCCTGCACGGCTCCAACGACGTGGTCAACGGCAAGCAC
ATCCGCATGGTCGACTTCGCCCTCTACATCGGCATCGCCATGTCGATCGCCGCCTTCCCC
GTGCTGGCGCGGATCATCACCGACCACCGGCTCCAGTTCACCCGGGTCGGCACGCTGGCC
CTGGCGCTCGCCGCCCTGGACGACATCCTCGCCTGGTCCATGCTGGCGGTGGTGGTGGCC
CTGGTCACGGCGTCCGGCACCGGCGGGTTCCTCAGCGTCATCGGCTGGGGCGCGGTCTAC
ATCGTGGTGATGCTGGTCATCGTCCGCCCCGCACTGGCCTACCTGGCCCGCAAGATCACC
AGCGCCTCCGCCCCCTACCTGGCGGTGGCCGCCGCGGCCGGCACCCTGTGCTCCGCCTAC
ATCACCTCGGAGATCGGCCTGCACGCCATCTTCGGGGCGTTCGTCTTCGGCCTGGTGATG
CCGCGCGGGCCGCAACCCGAGCTGATGCACCGGGCGGCCATGGTGCCGCTGGAGAACGTC
AGCAAGCTGCTGCTCCCCCTGTTCTTCGTGGTCACCGGACTGTCCGTGGACCTGACCGCG
ATGACCTCGGACGGGCTGATCCAGATGACCCTGATCATCTGCGTCGCCATCACCGGAAAG
CTGGGCGGCGTCCTGCTCTCGGCCAGGGTGACCGGCATGAGCTGGCAGGACTCCACCATC
CTCGGCCTGCTGATGAACACCCGGGGCCTGACCGAACTCGTCATCCTCAACGTCGGTTTG
AGCCTGGGTCTGCTGAGCGTCGAGCTGTTCACCGCAATGGTGATGATGGCGCTGGTCACC
ACGGGCATGACCTCCCCCATCCTGTCGATGATGCTCCGCAGGGCCGCGCCCCACTCGGAG
GCAGCAGTGCCCGCACACCTCAAGGACGGCGGCGACGAGTCCGACGCGTCAGCGGCGGCC
GTGCCGAGTGCCGCGAACGGCTCCGGCCCCGTGGCCGGCGTGGAGAACGGCGCCTCGCCC
GTGCACCGCGACGAGGCCGGCAGGAACAGCTATCTCCCCACCCCCCAGGGCGGGGACTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR006153 Cation/H+ exchanger (Domain) PF00999 Na_H_Exchanger
SignalP	Eukaryota
TMHMM	Transmembrane 10

Pyrlo_00050 Pyrlo_00070