Pyrlo_00080 : CDS information

Location

Organism	Actinosporangium vitaminophilum
Strain	ATCC 31673 (=NBRC 14294)
Entry name	Pyrrolomycin
Contig	EF140901
Start / Stop / Direction	9,376 / 10,788 / + [in whole cluster] 9,376 / 10,788 / + [in contig]
Location	9376..10788 [in whole cluster] 9376..10788 [in contig]
Type	CDS
Length	1,413 bp (470 aa)

Annotation

Category	1.4 Other
Product	putative L-proline--PCP ligase putative L-prolyl-PCP synthetase
Product (GenBank)	amino acid adenyltransferase
Gene
Gene (GenBank)	pyr8
EC number
Keyword	pyrrole ring moiety
Note
Note (GenBank)
Reference	ACC A3R4P9 PmId [17158935] Cloning and characterization of the pyrrolomycin biosynthetic gene clusters from Actinosporangium vitaminophilum ATCC 31673 and Streptomyces sp. strain UC 11065. (Antimicrob Agents Chemother. , 2007) comment pyrrolomycin biosynthetic gene clustersに関する文献。 pyr8(470 a.a.): adenylation of L-proline pyr8自体の機能実験は行われていないが、相同性よりL-prolineのadenylationに関与すると予測している。
Related Reference	ACC Q9X3R4 NITE Pyolu_00090 PmId [11880032] Conversion of L-proline to pyrrolyl-2-carboxyl-S-PCP during undecylprodigiosin and pyoluteorin biosynthesis. (Chem Biol. , 2002) comment Blast 13th, id49%, 1e-115 Pseudomonas fluorescens_pltF Putative acyl-CoA synthetase PltFのATP-[32P]PPi exchange活性測定。基質としてL-prolineに特異的。 holo-PCP(PltL)がPltFによってaminoacylateされることも確認。

PKS/NRPS Module

A	9..396

Sequence

>putative L-proline--PCP ligase [amino acid adenyltransferase]
MHSSEGPTTYRDLDRLADQYAAALLGRGVAPGDRVVIWSHKCTEAVAVMQAALRVGAIYI
PVTGSNPPARLTKIVANAEPALVVADEDAAFRAHKAGWDRAPLLSLDELRRKASEGARPK
PYENQPDEPAYILYTSGSTGDPKGVCISHRNSLAFVEWAAAEVAVGPQDRLSNHAPFNFD
LSVFDLYAAFRAGASVHLVPQEMAYAPEQLVQFMRDRGITVWYSVPSALSLMIREGGLLD
GPVPPALRACIFAGEPFAVHHVHALRKGWPQVRLLNWYGPTETNVCTWYEVTDADLERDR
PVPIGRACSGDTVMLDPDNGDEGEVVVSGPTVMLGYWGKEPHRGPYRTGDMARRNSDGDL
EYSGRRDHMVKVRGHRIELGEIEAAIASLDSVSDVSVLVVGSGLEAQLHAAVVSSQANRP
SLLTVKRACAERLPTYMIIDKLHILDDLPRTANGKTDRARLTAAAEAGEL

>putative L-proline--PCP ligase [amino acid adenyltransferase]
GTGCACAGCTCCGAGGGACCCACCACCTACCGCGACCTCGACCGCCTGGCGGACCAGTAC
GCCGCCGCCCTGCTGGGCCGCGGTGTGGCGCCCGGCGACCGGGTGGTGATCTGGAGCCAC
AAGTGCACCGAGGCGGTCGCCGTGATGCAGGCCGCACTGCGCGTCGGGGCCATCTACATA
CCGGTGACCGGCTCCAACCCGCCGGCCCGGCTGACCAAGATCGTGGCCAACGCGGAGCCG
GCCCTGGTCGTCGCCGACGAGGACGCGGCGTTCCGCGCGCACAAGGCCGGGTGGGACCGG
GCCCCGCTGCTCAGCCTGGACGAGCTGCGCCGCAAGGCCTCCGAGGGGGCCCGCCCCAAG
CCGTACGAGAACCAGCCGGACGAACCCGCGTACATCCTCTACACCTCCGGGTCCACCGGG
GACCCGAAGGGGGTGTGCATCAGCCACCGCAACTCCCTGGCGTTCGTGGAGTGGGCGGCG
GCCGAGGTCGCGGTCGGCCCGCAGGACCGGCTGTCCAACCACGCCCCGTTCAACTTCGAC
CTGTCGGTGTTCGACTTGTACGCCGCGTTCCGGGCCGGGGCCTCGGTGCACCTGGTGCCG
CAGGAGATGGCGTACGCCCCCGAGCAGTTGGTGCAGTTCATGCGGGACCGCGGGATCACC
GTGTGGTACTCGGTGCCGTCCGCGCTGAGCCTGATGATCCGTGAGGGCGGCCTGCTGGAC
GGGCCGGTGCCGCCGGCGCTGCGCGCCTGCATCTTCGCCGGCGAGCCGTTCGCCGTCCAC
CACGTGCACGCACTGCGCAAGGGCTGGCCGCAGGTCCGCCTGCTGAACTGGTACGGCCCC
ACCGAGACCAACGTCTGCACCTGGTACGAGGTGACCGATGCCGACCTGGAGCGCGACCGC
CCGGTGCCGATAGGGCGGGCCTGCTCTGGTGACACCGTCATGCTCGACCCGGACAACGGG
GACGAGGGCGAGGTCGTGGTGAGCGGCCCGACCGTCATGCTCGGCTACTGGGGCAAGGAA
CCGCACCGGGGTCCGTACCGCACGGGCGACATGGCCCGCCGGAACTCCGACGGGGACCTG
GAGTACTCGGGGCGGCGCGACCACATGGTCAAGGTGCGTGGACACCGGATCGAACTGGGC
GAGATCGAGGCGGCCATCGCCTCGCTGGACTCGGTGTCCGACGTGTCCGTACTGGTCGTC
GGCTCGGGGCTGGAGGCCCAGCTCCACGCGGCCGTCGTGTCCTCCCAGGCCAACCGTCCG
TCGTTGCTCACTGTCAAGCGCGCCTGCGCGGAAAGGCTGCCGACCTACATGATCATCGAC
AAGCTGCACATACTCGACGACCTGCCCAGGACAGCCAACGGCAAGACCGATCGCGCGCGG
CTCACCGCCGCCGCTGAGGCGGGTGAACTCTGA

[0] A	9..396

[0] A	25..1188

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000873 AMP-dependent synthetase/ligase (Domain) PF00501 AMP-binding IPR010071 Amino acid adenylation domain (Domain) TIGR01733 AA-adenyl-dom IPR020845 AMP-binding, conserved site (Conserved_site) PS00455 AMP_BINDING
SignalP	No significant hit
TMHMM	No significant hit

Pyrlo_00070 Pyrlo_00090