Pyrlo_00120 Pyrlo_00120   Pyrlo_00140 Pyrlo_00140

Pyrlo_00130 : CDS information

close this sectionLocation

Organism
StrainATCC 31673 (=NBRC 14294)
Entry namePyrrolomycin
Contig
Start / Stop / Direction15,643 / 14,861 / - [in whole cluster]
15,643 / 14,861 / - [in contig]
Locationcomplement(14861..15643) [in whole cluster]
complement(14861..15643) [in contig]
TypeCDS
Length783 bp (260 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative oxidoreductase
Product (GenBank)oxidoreductase
Gene
Gene (GenBank)pyr13
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[17158935] Cloning and characterization of the pyrrolomycin biosynthetic gene clusters from Actinosporangium vitaminophilum ATCC 31673 and Streptomyces sp. strain UC 11065. (Antimicrob Agents Chemother. , 2007)
comment
pyrrolomycin biosynthetic gene clustersに関する文献。

pyr13(260 a.a.): oxidoreductase

pyr13自体の機能実験は行われていないが、short-chain alcohol dehydrogenase関連の機能を持つと予測している。

同様の機能を持つと予測されているpyr15と共に、このうちの一方はdioxapyrrolomycinとpyrrolomycin G, Hに存在するpyrrole環とbenzene環に結合するC-6 keto groupをalcohol levelまで還元するのに機能し、もう一方はpyrrolomycin Bに存在するalcohol moietyを methylene groupにまで更に還元する機能を持つ可能性を記載している。
Family/Domain
FD
IPR002198
comment
[IPR002198] Short-chain dehydrogenase/reductase SDR (Domain)

Function
GO:0016491 oxidoreductase activity

close this sectionSequence

selected fasta
>putative oxidoreductase [oxidoreductase]
MTNSDHIKAAAQRVLEATDGRGAVGLVNNAGVGITSPTEIVPMATIRRSFEINFFGQLEM
IQEFLPQLRQTRGRLINIGSIGNRVVMPFAGPLNASKWALAAINHALRVELLPWGIHVVL
IEPASIKTAAVDKLEQDGLDNITQFSDHERFLYEDAYRSMLGNATMHVRRLGTSSEAVAK
AVHRSMVSPRPKARYLVGKSARPLAAVVKYAPDRLYDKVRLKVFALPKSFGARAGEAQPH
LMPQGTNQPKVATDGRRKAA
selected fasta
>putative oxidoreductase [oxidoreductase]
GTGACCAACAGCGACCACATCAAGGCAGCAGCGCAACGGGTCCTCGAGGCCACCGACGGA
CGCGGCGCCGTCGGTCTGGTGAACAACGCCGGCGTCGGCATCACCTCGCCGACCGAGATC
GTCCCCATGGCCACCATCCGGAGAAGCTTCGAGATCAACTTCTTCGGTCAGCTCGAGATG
ATCCAGGAGTTCCTGCCGCAACTCCGGCAGACCAGGGGACGCCTGATCAACATCGGGTCC
ATCGGCAACCGCGTGGTGATGCCCTTCGCCGGCCCGCTCAACGCGTCCAAGTGGGCGCTG
GCCGCGATCAACCACGCCCTGCGGGTGGAACTGCTCCCGTGGGGCATCCACGTGGTGCTG
ATCGAGCCGGCGTCGATCAAGACCGCGGCCGTGGACAAGCTGGAGCAGGACGGCCTCGAC
AACATCACCCAGTTCAGCGACCACGAGCGGTTCCTCTACGAGGACGCCTACCGCTCCATG
CTGGGCAACGCCACGATGCACGTCCGCCGGCTCGGCACCTCGTCCGAGGCCGTGGCCAAG
GCGGTGCACCGGTCGATGGTCTCGCCCCGGCCCAAGGCCCGCTACCTGGTGGGCAAGTCC
GCCCGACCGCTCGCCGCGGTGGTGAAGTACGCCCCCGACCGCCTGTACGACAAGGTGCGG
CTCAAGGTGTTCGCCCTGCCCAAGTCCTTCGGCGCCCGCGCCGGGGAGGCCCAGCCCCAC
CTGATGCCGCAGGGGACCAACCAGCCCAAGGTCGCCACCGACGGTCGCCGCAAGGCCGCC
TGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002198 Short-chain dehydrogenase/reductase SDR (Family)
[9-112] 2.80000000000001e-09 PF00106
PF00106   adh_short
IPR002347 Glucose/ribitol dehydrogenase (Family)
[21-32] 2.10000037262656e-06 PR00081 [67-83] 2.10000037262656e-06 PR00081 [93-112] 2.10000037262656e-06 PR00081 [114-131] 2.10000037262656e-06 PR00081
PR00081   GDHRDH
IPR016040 NAD(P)-binding domain (Domain)
[2-223] 1.9e-36 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP No significant hit
TMHMM No significant hit
Pyrlo_00120 Pyrlo_00120   Pyrlo_00140 Pyrlo_00140
Page top