Pyrlo_00180 Pyrlo_00180   Pyrlo_00200 Pyrlo_00200

Pyrlo_00190 : CDS information

close this sectionLocation

Organism
StrainATCC 31673 (=NBRC 14294)
Entry namePyrrolomycin
Contig
Start / Stop / Direction24,575 / 25,996 / + [in whole cluster]
24,575 / 25,996 / + [in contig]
Location24575..25996 [in whole cluster]
24575..25996 [in contig]
TypeCDS
Length1,422 bp (473 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative assimilatory nitrate reductase electron transfer subunit
Product (GenBank)assimilatory nitrate reductase electron transfer subunit
Gene
Gene (GenBank)pyr19
EC number
Keyword
Note
  • This ORF product lacks the BFD-like [2Fe-2S]-binding domain (IPR007419) in the C-terminal region.
Note (GenBank)
Reference
ACC
PmId
[17158935] Cloning and characterization of the pyrrolomycin biosynthetic gene clusters from Actinosporangium vitaminophilum ATCC 31673 and Streptomyces sp. strain UC 11065. (Antimicrob Agents Chemother. , 2007)
comment
pyrrolomycin biosynthetic gene clustersに関する文献。

pyr19(473 a.a.): assimilatory nitrate reductase, electron transfer subunit

pyr19自体の機能実験は行われていないが、相同性よりassimilatory nitrate reductase, electron transfer subunitと予測している。pyr18と共にnitrateを還元し、その結果供給されるnitriteがnitrated pyrrolomycinの生合成に使われると推測されている。
Related Reference
ACC
Q9EYG1
PmId
[21424691] Identification and functional analysis of a nitrate assimilation operon nasACKBDEF from Amycolatopsis mediterranei U32. (Arch Microbiol. , 2011)
comment
<Assimilatory nitrate reductase electron transfer subunit(nasC), Amycolatopsis mediterranei, BLAST 3, id=52%>

nasA null mutant, nasC null mutantでは、それぞれnitrateを唯一のN源とした培地で生育できないが、nitriteを唯一のN源とした場合は生育できること、また、 nasA null mutantはnasACによって相補されることが示されており、nasA, nasC共にassimilatory nitrate reductaseに不可欠であることが示されている。

close this sectionSequence

selected fasta
>putative assimilatory nitrate reductase electron transfer subunit [assimilatory nitrate reductase electron transfer subunit]
MVIVGNGMAGHRLAQELRARRPEVRVTLVGEEPRPAYNRVLLSTVLAGTVSADALELADC
GARMRLGVRALELDTERRRVTLSDGSVLGYETLVLATGARPVLPRVPGLAADDGGPAPGV
STFRTLADCTLISRLALGGSMAVLGGGLLGVEAARGLAGLGVDVTLVHPVGHLMENQLDP
EAGEVLARMLRDLGVRVLLGVGASRWQPSPDEGELVLSDGRAVRCGGVVVTAGVAPVTEL
ARSAGLAVGRGVVVDDRLRTSAPGVYAIGECAEHRGVCHGLVGPCWEQAAVLADVLVGKD
TEYTGSRPVVRLKARDVDLAAMGRVPAEPDGDTELVRVSDVTRGRYGVLALRAERLVGAI
LLGLPDATGPVTQLHDSGSPAPADRFGLLLGTGAAVSETPDKLPERALVCRCNGVTKARL
VEAWHAGARDPEALSVLHLGHHRLRQLFGHRAAAVHLAGARGRLTVHGRPGRP
selected fasta
>putative assimilatory nitrate reductase electron transfer subunit [assimilatory nitrate reductase electron transfer subunit]
GTGGTCATCGTCGGCAACGGCATGGCCGGGCACCGGCTCGCCCAGGAGCTGCGCGCCCGG
CGCCCGGAGGTGCGGGTCACCCTGGTGGGCGAGGAGCCGCGCCCCGCCTACAACCGGGTG
CTGCTGTCCACCGTCCTCGCCGGCACCGTGTCCGCGGACGCCCTGGAGCTCGCCGACTGC
GGTGCCCGGATGCGCCTGGGCGTGCGGGCGCTCGAGCTCGACACGGAGCGCCGCCGGGTC
ACCCTCTCCGACGGGTCGGTCCTCGGCTACGAGACGCTGGTGCTGGCCACGGGGGCGCGC
CCGGTGCTGCCCCGGGTGCCGGGGCTCGCCGCCGACGACGGCGGACCCGCGCCCGGGGTG
AGCACCTTCCGCACCCTGGCGGACTGCACGCTCATCTCCCGCCTCGCCCTGGGCGGGTCC
ATGGCCGTGCTCGGGGGCGGGCTGCTCGGGGTGGAGGCGGCCCGGGGCCTGGCCGGGCTG
GGGGTGGACGTCACCCTGGTGCACCCGGTGGGTCACCTGATGGAGAACCAGCTCGACCCC
GAGGCCGGGGAGGTCCTCGCCCGGATGCTGCGTGACCTGGGCGTGCGGGTGCTGCTCGGC
GTGGGTGCCAGCCGTTGGCAGCCGTCGCCGGACGAGGGCGAACTCGTCCTGTCCGACGGC
CGGGCGGTGCGGTGCGGCGGGGTCGTGGTGACCGCGGGGGTGGCCCCGGTGACCGAGCTG
GCCCGCTCGGCGGGGCTGGCCGTGGGACGGGGCGTCGTGGTGGACGACCGGCTGCGGACC
TCCGCGCCCGGGGTGTACGCGATCGGCGAGTGCGCCGAGCACCGGGGTGTGTGCCACGGT
CTGGTGGGGCCGTGCTGGGAGCAGGCCGCCGTCCTCGCCGACGTGCTCGTCGGCAAGGAC
ACCGAGTACACCGGGTCCCGTCCGGTGGTGCGGCTCAAGGCCCGCGACGTGGACCTGGCC
GCGATGGGTCGTGTCCCCGCGGAGCCGGACGGGGACACCGAGCTGGTGCGGGTGTCCGAT
GTCACCCGAGGCCGCTACGGCGTTCTCGCGCTGCGCGCCGAACGTCTCGTCGGGGCGATC
CTGCTCGGGCTGCCGGACGCCACCGGGCCGGTCACCCAGCTCCATGACTCCGGGTCGCCG
GCACCCGCCGACCGCTTCGGGTTGCTGCTCGGGACCGGGGCCGCGGTCAGCGAGACGCCG
GACAAGCTGCCGGAACGGGCGCTGGTGTGCCGGTGCAACGGGGTGACCAAGGCCCGGCTC
GTCGAGGCCTGGCACGCCGGTGCCCGCGACCCCGAGGCGCTGTCCGTCCTCCACCTGGGC
CACCACCGGCTGCGGCAGTTGTTCGGACACCGTGCAGCGGCTGTGCACCTGGCTGGCGCG
CGAGGCCGTCTGACCGTCCATGGCCGCCCCGGGCGGCCGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001327 Pyridine nucleotide-disulphide oxidoreductase, NAD-binding domain (Domain)
[140-212] 2e-14 PF00070
PF00070   Pyr_redox
IPR013027 FAD-dependent pyridine nucleotide-disulphide oxidoreductase (Family)
[1-14] 9.19998414420358e-24 PR00368 [90-108] 9.19998414420358e-24 PR00368 [140-158] 9.19998414420358e-24 PR00368 [225-241] 9.19998414420358e-24 PR00368 [250-272] 9.19998414420358e-24 PR00368
PR00368   FADPNR
IPR023753 Pyridine nucleotide-disulphide oxidoreductase, FAD/NAD(P)-binding domain (Domain)
[2-114] 4.00000000000001e-13 PF07992 [231-274] 9.9e-07 PF07992
PF07992   Pyr_redox_2
SignalP
[1-38] 0.04 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Pyrlo_00180 Pyrlo_00180   Pyrlo_00200 Pyrlo_00200
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