Stref_00030 Stref_00030   Stref_00050 Stref_00050

Stref_00040 : CDS information

close this sectionLocation

Organism
StrainNRRL 3193 (=NBRC 13446)
Entry nameSteffimycin
Contig
Start / Stop / Direction3,788 / 3,102 / - [in whole cluster]
3,788 / 3,102 / - [in contig]
Locationcomplement(3102..3788) [in whole cluster]
complement(3102..3788) [in contig]
TypeCDS
Length687 bp (228 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative ribulose-phosphate 3-epimerase
Product (GenBank)putative ribulose-phosphate 3-epimerase
Gene
Gene (GenBank)
EC number5.1.3.1
Keyword
Note
  • It is supposed that this ORF is not involved in steffimycin biosynthesis.
Note (GenBank)
  • ORF4
Reference
ACC
PmId
[16751529] Isolation, characterization, and heterologous expression of the biosynthesis gene cluster for the antitumor anthracycline steffimycin. (Appl Environ Microbiol. , 2006)
comment
steffimycin生合成gene clusterのクローニング、特徴づけ。

36ORFsが見つかり、そのうち24ORFsを含む26.5 kb領域がおそらくsteffimycin生合成に関連し、一次代謝関連genesとhousekeeping genesを含む12ORFsによって挟まれている。

orf1-4, 33-36は、S. coelicolor A3(2) and S. avermitilis MA-4680由来の関連タンパクと有意な類似性あり。遺伝子構成も同じ。

orf4はsteffimycin生合成には関係ないとされる12ORFsに含まれるが、異種性steffimycin発現のためのplasmid pEM4STFaには含まれている。
Related Reference
ACC
P46969
PmId
[8929392] ( , )
[8879247] ( , )
comment
Blast 339th, id44%, 4e-50
Saccharomyces cerevisiae (Baker's yeast)_RPE1
Ribulose-phosphate 3-epimerase(EC 5.1.3.1)

---
[PMID: 8929392](1996)
ribulose-phosphate 3-epimerase(EC5.1.3.1)(rpe1p)
D-ribose-5-phosphate ketol-isomerase (rki1p)
のgene cloningとprotein characterization。

rpe1pは抽出液に高い特異的活性あり。
RPE1 deletion mutantは酵素活性なし。D-xylulose下で生育不可。

---
[PMID: 8879247](1996)
酸化ストレス感受性mutantを分離。5-phosphate epimerase activity が見られなかったことから、対応する構造遺伝子がこれをコードすると推測しRPE1とした。

molecular mass、codon adaptation index (CAI)、isoelectric point、homology解析。
rpe1 deletion mutants と RPE1を強発現する株で酵素活性を測定し、hydrogen peroxide sensitivityを検討している。

close this sectionSequence

selected fasta
>putative ribulose-phosphate 3-epimerase [putative ribulose-phosphate 3-epimerase]
MAVQINPSILSADFARLADEAKAVEGADWLHVDVMDNHFVPNLTLGVPVVESLARATDTP
LDCHLMIEAPDRWAPQYVEAGAGSVTFHVEAAAAPVRLAREIRAKGARASMALKPATPIE
PYEDLLPELDMLLIMTVEPGFGGQAFLDIMLPKIRRTRELISKHGLELWLQVDGGVSAST
IERCADAGADVFVAGSAVYGANDPAEAVRALRTQAEAITAKASWACDH
selected fasta
>putative ribulose-phosphate 3-epimerase [putative ribulose-phosphate 3-epimerase]
ATGGCCGTGCAGATCAACCCCAGCATCCTGTCCGCAGACTTCGCCCGCCTCGCGGACGAG
GCGAAGGCCGTGGAGGGAGCCGACTGGCTCCACGTCGACGTCATGGACAACCATTTCGTC
CCGAACCTCACGCTCGGCGTGCCGGTCGTCGAGTCGCTGGCCCGTGCGACGGACACCCCG
CTGGATTGCCATCTGATGATCGAGGCCCCCGACCGGTGGGCGCCCCAGTACGTGGAGGCG
GGGGCCGGTTCCGTCACCTTCCACGTCGAGGCCGCCGCCGCCCCGGTCCGGCTCGCCCGG
GAGATCCGCGCCAAGGGTGCCCGCGCCTCCATGGCGCTGAAGCCCGCGACCCCCATCGAG
CCGTACGAGGACCTGCTCCCCGAGCTCGACATGCTGCTGATCATGACGGTCGAACCGGGC
TTCGGGGGACAGGCCTTTCTCGACATCATGCTTCCGAAGATTCGCCGCACCCGCGAGTTG
ATCAGCAAGCACGGCTTGGAGCTCTGGCTCCAGGTCGACGGCGGAGTCTCCGCTTCCACC
ATCGAGCGCTGCGCCGACGCGGGAGCCGACGTCTTCGTGGCCGGATCGGCGGTCTACGGG
GCAAACGACCCGGCAGAGGCGGTACGTGCACTACGCACACAGGCGGAGGCGATCACGGCC
AAGGCCTCCTGGGCCTGCGACCACTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000056 Ribulose-phosphate 3-epimerase-like (Family)
[5-212] 1e-81 TIGR01163
TIGR01163   Rpe
[132-154] PS01086
PS01086   RIBUL_P_3_EPIMER_2
[5-201] 2.50000000000001e-79 PF00834
PF00834   Ribul_P_3_epim
[30-44] PS01085
PS01085   RIBUL_P_3_EPIMER_1
IPR011060 Ribulose-phosphate binding barrel (Domain)
[1-218] 1.90000694315261e-66 SSF51366
SSF51366   RibP_bind_barrel
IPR013785 Aldolase-type TIM barrel (Domain)
[4-221] 1.60000000000002e-90 G3DSA:3.20.20.70
G3DSA:3.20.20.70   Aldolase_TIM
IPR026019 Ribulose-phosphate 3-epimerase (Family)
[1-224] PIRSF001461
PIRSF001461   RPE
SignalP
[1-23] 0.121 Signal
Eukaryota   
[1-23] 0.302 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Stref_00030 Stref_00030   Stref_00050 Stref_00050
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