Stref_00170 : CDS information

Location

Organism	Streptomyces steffisburgensis
Strain	NRRL 3193 (=NBRC 13446)
Entry name	Steffimycin
Contig	AM156932
Start / Stop / Direction	15,987 / 15,424 / - [in whole cluster] 15,987 / 15,424 / - [in contig]
Location	complement(15424..15987) [in whole cluster] complement(15424..15987) [in contig]
Type	CDS
Length	564 bp (187 aa)

Annotation

Category	5.4 hypothetical protein
Product	hypothetical protein
Product (GenBank)	SWIM Zn-finger
Gene
Gene (GenBank)	stfC
EC number
Keyword
Note
Note (GenBank)
Reference	ACC Q2P9Z8 PmId [16751529] Isolation, characterization, and heterologous expression of the biosynthesis gene cluster for the antitumor anthracycline steffimycin. (Appl Environ Microbiol. , 2006) comment steffimycin生合成gene clusterのクローニング、特徴づけ。 36ORFsが見つかり、そのうち24ORFsを含む26.5 kb領域がおそらくsteffimycin生合成に関連する。 stfC: Unknown 配列解析のみ。 SWIM-like zinc-chelating domainsを含むhypoタンパクに似ている。

Sequence

>hypothetical protein [SWIM Zn-finger]
MMPAEFGATPWGRIWVRTVESTAAAGPNPALPKARSLARNHAATLTVETGRVEAEVVVSG
RTQQVRIDLPPWSDQERAEAERLVGKALAEHQNLAAGDLPDSLEADLRREKIGVAVALDD
MDAACDCRTRRRPCVHVLATLYTLSQRIDERPLLAVELRSPGWEPAAPADPDWTPLADLD
VESFYGP

>hypothetical protein [SWIM Zn-finger]
CTGATGCCGGCCGAATTCGGAGCCACCCCCTGGGGCAGGATCTGGGTACGAACCGTGGAG
TCGACGGCGGCGGCCGGGCCGAACCCGGCGCTGCCGAAGGCGAGGAGTCTGGCGCGCAAC
CATGCCGCCACGCTGACCGTCGAGACCGGACGCGTCGAAGCCGAGGTCGTGGTCTCGGGC
AGGACCCAGCAGGTCCGGATCGACCTGCCGCCCTGGTCCGATCAGGAGCGTGCGGAAGCC
GAACGCCTCGTCGGCAAGGCGCTGGCCGAGCACCAGAACCTTGCCGCCGGAGACCTGCCC
GACAGCCTCGAGGCGGACCTCCGGCGCGAGAAGATCGGCGTGGCCGTGGCCCTCGACGAC
ATGGACGCCGCCTGCGACTGCCGGACGCGGCGCAGGCCCTGCGTCCATGTGCTGGCCACC
CTCTACACCTTGTCCCAGCGCATCGACGAGCGTCCGCTGCTGGCCGTCGAGTTGCGCTCC
CCGGGGTGGGAGCCCGCCGCTCCCGCGGATCCCGACTGGACGCCGTTGGCCGACCTCGAC
GTGGAGTCGTTCTACGGACCCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR007527 Zinc finger, SWIM-type (Domain) PF04434 SWIM PS50966 ZF_SWIM
SignalP	Bacteria, Gram-negative
TMHMM	No significant hit

Stref_00160 Stref_00180