Strply_00090 Strply_00090   Strply_00110 Strply_00110

Strply_00100 : CDS information

close this sectionLocation

Organism
StrainNRRL 2433 (=NBRC 13058)
Entry nameStreptolydigin
Contig
Start / Stop / Direction12,755 / 11,739 / - [in whole cluster]
12,755 / 11,739 / - [in contig]
Locationcomplement(11739..12755) [in whole cluster]
complement(11739..12755) [in contig]
TypeCDS
Length1,017 bp (338 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative NDP-hexose C-3 ketoreductase
Product (GenBank)NDP-hexose-3-ketoreductase
Gene
Gene (GenBank)slgS4
EC number
Keyword
  • L-rhodinose
Note
Note (GenBank)
Reference
ACC
PmId
[19875077] Deciphering biosynthesis of the RNA polymerase inhibitor streptolydigin and generation of glycosylated derivatives. (Chem Biol. , 2009)
comment
streptolydigin生合成gene clusterの報告。

上流ORFの挿入変異株の解析より、本ORFはstreptolydigin生産に関与する領域(PKS-NRPS system)であることが示されている。

本ORFの機能解析はしていない。

streptolydigin誘導体を検出するためL-rhodinose biosynthesisに関与するslg3からslg7までの遺伝子同時欠損株(SLM7H13)を作製。
検出された2つの化合物(streptolydiginone,demethyl-streptolydiginone)の産生は、糖鎖形成の前にエポキシ化とメチル化が起こることを示した。
slg3-slg7を含むプラスミドの挿入によりstreptolydigin生合成能の回復を確認している。
Related Reference
ACC
Q9ALN5
NITE
Spino_00080
PmId
[18345667] In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa. (J Am Chem Soc. , 2008)
comment
Saccharopolyspora spinosa_dTDP-hexose 3-ketoreductase(spnN)

PMID: 18345667
NAD(P)H-dependent dTDP-3,4-diketo-2,6-dideoxy-D-glucose 3-ketoreductase_SpnN

dTDP-D-glucose, RfbB(dTDP-D-glucose→dTDP-4-keto-6-deoxy-D-glucose), TylX3(SpnNの1つ前の反応を担うSpnOのhomologue), SpnN, and NADPHをインキュベーションしてできた産物がdTDP-4-keto-2,6-dideoxy-D-glucoseであることをHPLC chromatogram、1H NMR spectrumなどで確認している。NADPHはNADHの2倍速く消費されるので、おそらくNADPH-dependentである。

close this sectionSequence

selected fasta
>putative NDP-hexose C-3 ketoreductase [NDP-hexose-3-ketoreductase]
MSTVADPQHREPPLRFGVLGCADIARRRVLPALLRTPGARLTAVASRDSARARQVADTFG
CAPVAGYQALLARDDIDAVYLPLPPALHPEWIRRALTAGKHVLAEKPLTTSSAETAELTA
LAAASGRVLAENVMFVHHPRHERVRRLVADGAVGVPQTFTAAFTVPRRPPGDIRLSAELG
GGALLDIGGYPLRAAQYFLGPDLRVAGAALRTDGAAEVDCGGSALLVAGDGTVAALTFGL
DHAYVSRYEILGSTGRITVDRAFTPPADHVGEIRLDRADGTETLPTPAADQCVNTLAAFV
RAVRTGETAAGHGTMRRQAELIEEIRRAAGMTDREPGR
selected fasta
>putative NDP-hexose C-3 ketoreductase [NDP-hexose-3-ketoreductase]
ATGAGCACCGTGGCCGATCCGCAGCACCGTGAACCCCCGCTCCGCTTCGGCGTCCTGGGC
TGTGCCGACATCGCCCGGCGCCGGGTGCTGCCGGCCCTGCTCCGGACCCCCGGCGCCCGG
CTCACCGCCGTCGCCAGCCGCGATTCCGCCCGTGCCCGGCAGGTCGCCGACACCTTCGGC
TGCGCCCCGGTGGCCGGGTACCAGGCCCTGCTGGCACGCGACGACATCGACGCCGTGTAC
CTGCCCCTGCCCCCGGCCCTGCACCCGGAGTGGATCCGGCGCGCCCTCACGGCCGGCAAG
CACGTCCTCGCCGAGAAGCCGCTCACCACGAGCAGCGCCGAGACCGCCGAACTGACCGCG
CTGGCCGCCGCCTCGGGACGGGTGCTGGCCGAGAACGTGATGTTCGTGCACCACCCGCGG
CACGAACGGGTACGCCGGCTCGTCGCCGACGGCGCCGTCGGGGTGCCGCAGACGTTCACC
GCCGCCTTCACCGTCCCCCGGCGGCCGCCGGGCGACATCCGGCTCTCCGCGGAGCTGGGC
GGCGGCGCGCTGCTGGACATCGGCGGCTATCCGCTGCGCGCCGCCCAGTACTTCCTCGGC
CCGGACCTGCGGGTCGCCGGCGCGGCCCTGCGCACCGACGGGGCGGCCGAAGTCGACTGC
GGGGGCAGCGCGTTGCTGGTCGCCGGCGACGGCACGGTGGCCGCGCTTACGTTCGGCCTC
GACCACGCCTATGTGAGTCGCTACGAGATTCTCGGCAGCACCGGCCGGATCACCGTCGAC
CGCGCCTTCACCCCGCCCGCCGACCACGTCGGAGAGATCCGGCTGGACCGCGCCGACGGC
ACCGAAACCCTCCCCACCCCCGCCGCCGACCAGTGCGTCAACACCCTCGCCGCCTTCGTC
CGCGCCGTACGCACCGGTGAAACGGCCGCCGGGCACGGGACGATGCGGCGCCAGGCGGAG
CTGATCGAGGAGATCCGCCGCGCCGCCGGGATGACGGACCGGGAGCCGGGCCGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000683 Oxidoreductase, N-terminal (Domain)
[14-130] 4.9e-27 PF01408
PF01408   GFO_IDH_MocA
IPR004104 Oxidoreductase, C-terminal (Domain)
[145-250] 3.4e-07 PF02894
PF02894   GFO_IDH_MocA_C
IPR016040 NAD(P)-binding domain (Domain)
[11-155] 3.89999999999997e-43 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP
[1-45] 0.335 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Strply_00090 Strply_00090   Strply_00110 Strply_00110
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