Strply_00120 Strply_00120   Strply_00140 Strply_00140

Strply_00130 : CDS information

close this sectionLocation

Organism
StrainNRRL 2433 (=NBRC 13058)
Entry nameStreptolydigin
Contig
Start / Stop / Direction16,193 / 15,339 / - [in whole cluster]
16,193 / 15,339 / - [in contig]
Locationcomplement(15339..16193) [in whole cluster]
complement(15339..16193) [in contig]
TypeCDS
Length855 bp (284 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative glycosidase
Product (GenBank)putative secreted hydrolase
Gene
Gene (GenBank)slgC2
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[19875077] Deciphering biosynthesis of the RNA polymerase inhibitor streptolydigin and generation of glycosylated derivatives. (Chem Biol. , 2009)
[22726958] Participation of putative glycoside hydrolases SlgC1 and SlgC2 in the biosynthesis of streptolydigin in Streptomyces lydicus. (Microb Biotechnol. , 2012)
comment
[PMID:19875077]
streptolydigin生合成gene clusterの報告。

glycosyl hydrolase familyに属するORF

endo-1,3-beta-glucanasesと相同性を示すが、実験はされていない。

[PMID:22726958(abstractのみ)]
SlgC1,SlgC2の機能解析の報告。

slgC1,slgC2の不活性株はそれぞれstreptolydigin生産に影響有り。
slgC1,slgC2の過剰発現株はそれぞれstreptolydigin生産増加した。したがってSlgC1,SlgC2はstreptolydigin生合成に関与する。
Related Reference
ACC
Q2V0H0
PmId
[16601914] Molecular identification of a novel beta-1,3-glucanase from alkaliphilic Nocardiopsis sp. strain F96. (Extremophiles. , 2006)
comment
Nocardiopsis sp. F96
Beta-1,3-glucanase(bglF)

この酵素はinsoluble beta-1,3-glucanを加水分解し、beta-1,3-glucanよりもbeta-1,3-1,4-glucanに対して最も高い活性を示したことから、BglFは一般的なbeta-1,3-glucanaseとしている。
また、mutant解析から、触媒残基はGlu123とGlu128であることを明らかにしている。

close this sectionSequence

selected fasta
>putative glycosidase [putative secreted hydrolase]
MTTADPKDRTFSATQWRVLLHDDFTGPAGSPPDAGRWLTRTGRPFGAGVETHADDSGHAA
LDGAGRLAMTATRDASGGFTAAWLESRREDLLPPSGGALRIQARVRTAPGPGLDCALWAW
GNPMRHPEPGEAELDRWFRAGEMDVFEVLGSQPDRVHGSLHSPACDQLPSCGIGGHGATV
DGAPLSDGFHTYSMVWTRDPDTVTWHIDARPYLKLTPEDTTPEGWLFNQPAHLCLAIIIG
SPGGPVPPGTPDPAAFPAAMLVDEITVAVRGPKGGTDAAGEAPR
selected fasta
>putative glycosidase [putative secreted hydrolase]
ATGACGACAGCCGATCCCAAAGATCGCACCTTTTCCGCGACGCAGTGGCGCGTCCTGTTG
CACGACGACTTCACGGGCCCGGCCGGCTCCCCGCCCGACGCCGGACGCTGGCTGACGCGC
ACCGGCCGCCCCTTCGGCGCCGGGGTCGAGACGCATGCCGACGACAGCGGTCATGCGGCG
CTCGACGGCGCCGGCCGGCTGGCGATGACCGCCACCCGCGACGCCTCGGGCGGCTTCACG
GCTGCCTGGCTCGAAAGCCGCCGGGAGGACCTGCTGCCGCCCAGCGGCGGAGCCCTGCGC
ATCCAGGCGCGGGTCCGCACCGCCCCGGGGCCCGGCCTGGACTGCGCCCTGTGGGCCTGG
GGCAACCCGATGCGGCACCCGGAGCCCGGTGAGGCCGAACTGGACCGCTGGTTCCGGGCG
GGCGAGATGGACGTGTTCGAGGTGCTCGGCTCCCAACCCGACCGGGTCCACGGCTCGTTG
CACTCCCCGGCCTGCGACCAGCTGCCCTCCTGCGGCATCGGCGGACACGGCGCGACCGTG
GACGGCGCACCGCTGAGCGACGGCTTCCACACCTACTCCATGGTCTGGACCCGCGACCCG
GACACCGTCACCTGGCACATCGACGCCCGCCCCTACCTCAAGCTGACCCCCGAGGACACC
ACCCCGGAGGGCTGGCTCTTCAACCAGCCGGCCCACCTGTGCCTGGCCATCATCATCGGC
AGCCCGGGCGGCCCGGTGCCGCCGGGCACGCCGGACCCCGCCGCCTTCCCCGCCGCCATG
CTCGTCGACGAGATCACCGTCGCGGTGCGCGGCCCCAAGGGCGGCACCGACGCGGCCGGG
GAGGCTCCGCGATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000757 Glycoside hydrolase, family 16 (Domain)
[100-239] 1.3e-11 PF00722
PF00722   Glyco_hydro_16
IPR008985 Concanavalin A-like lectin/glucanase (Domain)
[5-275] 9.49996855930963e-33 SSF49899
SSF49899   ConA_like_lec_gl
IPR013320 Concanavalin A-like lectin/glucanase, subgroup (Domain)
[12-268] 4.39999999999997e-33 G3DSA:2.60.120.200
G3DSA:2.60.120.200   ConA_like_subgrp
SignalP No significant hit
TMHMM No significant hit
Strply_00120 Strply_00120   Strply_00140 Strply_00140
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