Strply_00220 : CDS information

Location

Organism	Streptomyces lydicus
Strain	NRRL 2433 (=NBRC 13058)
Entry name	Streptolydigin
Contig	FN433113
Start / Stop / Direction	26,650 / 28,749 / + [in whole cluster] 26,650 / 28,749 / + [in contig]
Location	26650..28749 [in whole cluster] 26650..28749 [in contig]
Type	CDS
Length	2,100 bp (699 aa)

Annotation

Category	4.1 transcriptional regulator
Product	regulatory protein
Product (GenBank)	putative ATP/GTP binding-protein
Gene
Gene (GenBank)	slgY
EC number
Keyword
Note
Note (GenBank)
Reference	ACC D1GLV0 PmId [19875077] Deciphering biosynthesis of the RNA polymerase inhibitor streptolydigin and generation of glycosylated derivatives. (Chem Biol. , 2009) [22820841] Three pathway-specific regulators control streptolydigin biosynthesis in Streptomyces lydicus. (Microbiology. , 2012) comment [PMID:19875077] streptolydigin生合成gene clusterの報告。このORFは機能解析実験されていない。 [PMID:22820841] ３つのreguratory proteinの機能解析報告。 slgR1 TetR transcriptional regulator families slgR2 LuxR transcriptional regulator families　 slgY ATP/GTP-binding proteins of DNA and RNA helicase superfamily I <-this ORF slgR1 or slgR2を不活化するとstreptolydiginを産生出来ないので、SlgR1 と SlgR2はpathway-specific positive regulatorである。 slgYはslgR2の発現を制御する。

Sequence

>regulatory protein [putative ATP/GTP binding-protein]
MTSYAQDAYTPDSRAGDATGLDDELAEERRYVALCRSAMTRQVEDAGLQVTEGQDVSGDG
ASAEALGRYLRTRARDMAQEPDSPLFFGRLDFDDAPETGDHRRQRYYIGRRRVSEHPAAP
PLVVDWRAPVSRTFYQASALEPQGVAVRRRFGWAPLSSGEPKDLTGMEDEHLDRQEDVPA
GASRIVAAEIERPRTGPMRDIAATIQPEQDDLVRRELADSVCIQGAPGSGKTAVGLHRAA
YLLYTYPQRLQRSGLLIIGPNQTFLRYIAQVLPALGEIDIAQCTVEDVVARHPVQAVDGE
LAAAVKQSHRMAAVLERALYSRVTAVPDGITVPDGSYRWRVYGDELSRIVEEVREQAPPY
AVGREHVRSRAVSLVQEQAERRAGPKNMTWQRKIGRCRPVAAFLDAVWPTVRAEEVVAGL
LGDAEALARAADGLLDADEQAALVWDRPPRSVKSAKWSVQDMLLIDEVAGLIERPGSFGH
VVVDEAQDLSPMQCRAIARRSGFGSLTVLGDLAQATAPWAARSWAEQLAHLGKTQGTVTE
LTTGFRVPAAVMSLANRLLGALDVDVPPARSLRQDGELSVRRVPDTLSATVEAARQALTQ
EGAIAVITADAEAERTAEALRAAGLEVATSEDVGTAARLAVLPAKVVKGLEYDHVIVVEP
SAIVAAEPRGLHRLYVAVTRAVSRLDVLHAHPLPEPLAG

>regulatory protein [putative ATP/GTP binding-protein]
ATGACGTCATACGCCCAGGACGCGTACACCCCGGACTCCCGCGCCGGCGATGCCACCGGG
CTGGACGACGAACTCGCCGAGGAGCGGCGGTACGTCGCCCTGTGCCGGAGCGCGATGACC
CGGCAGGTGGAGGACGCGGGCCTGCAGGTCACCGAGGGGCAGGACGTCTCGGGCGACGGC
GCGAGCGCGGAGGCGCTGGGCCGGTATCTGCGCACCCGCGCACGGGACATGGCGCAGGAG
CCGGACAGCCCGCTCTTCTTCGGCCGGCTGGACTTCGACGACGCGCCGGAGACCGGGGAC
CACCGCCGGCAGCGCTACTACATCGGGCGCCGCCGGGTCTCGGAGCACCCGGCCGCGCCG
CCGCTGGTCGTCGACTGGCGGGCGCCGGTCTCGCGCACCTTCTATCAGGCGAGCGCCCTG
GAACCGCAGGGCGTCGCGGTCCGCCGGCGCTTCGGCTGGGCACCGCTGAGCAGCGGCGAG
CCGAAGGACCTGACGGGCATGGAGGACGAGCACCTCGACCGGCAGGAGGACGTGCCGGCC
GGGGCGAGCCGGATCGTCGCGGCCGAGATCGAGCGCCCGCGCACCGGCCCGATGCGCGAC
ATCGCCGCCACCATCCAGCCGGAGCAGGACGACCTGGTGCGCCGCGAACTGGCCGATTCG
GTGTGCATCCAGGGGGCACCGGGCAGCGGAAAGACCGCGGTCGGTCTGCACCGTGCGGCC
TACCTGCTGTACACCTACCCGCAGCGGCTGCAGCGCAGCGGTCTGCTGATCATCGGCCCC
AACCAGACGTTCCTGCGCTACATCGCCCAGGTGCTCCCGGCGCTCGGCGAGATCGACATC
GCGCAGTGCACGGTGGAGGACGTGGTGGCCCGCCATCCGGTGCAGGCCGTCGACGGCGAA
CTGGCGGCGGCCGTCAAGCAGAGCCACCGGATGGCGGCCGTACTGGAGCGCGCCCTGTAC
AGCAGGGTCACCGCGGTCCCCGACGGCATCACCGTCCCGGACGGCTCCTACCGCTGGCGG
GTGTACGGCGACGAGTTGAGCCGCATCGTCGAGGAGGTCCGCGAGCAGGCGCCGCCGTAC
GCGGTGGGGCGCGAACACGTCCGCTCGCGGGCGGTGTCGCTGGTGCAGGAGCAGGCCGAG
CGCCGGGCGGGCCCCAAGAACATGACCTGGCAGCGGAAGATCGGCCGCTGCCGCCCGGTC
GCGGCCTTCCTGGACGCCGTGTGGCCCACGGTCCGGGCGGAGGAGGTGGTCGCCGGTCTG
CTGGGCGATGCCGAGGCGCTGGCCCGGGCGGCGGACGGGCTGCTGGACGCGGACGAGCAG
GCGGCGCTGGTGTGGGACAGGCCGCCGCGATCGGTCAAGAGCGCCAAGTGGTCCGTCCAG
GACATGCTGTTGATCGACGAGGTAGCCGGCCTGATCGAGCGGCCCGGCAGCTTCGGCCAT
GTGGTCGTCGACGAGGCCCAGGACCTCTCCCCCATGCAGTGCCGGGCGATCGCCCGCCGC
AGCGGCTTCGGTTCGCTCACCGTACTGGGTGACCTGGCACAGGCCACCGCTCCGTGGGCG
GCGCGCTCCTGGGCCGAGCAGCTGGCGCATCTGGGCAAGACCCAGGGGACGGTCACCGAG
CTCACCACCGGCTTCCGGGTGCCGGCCGCCGTGATGTCCCTGGCCAACCGGCTGCTGGGC
GCGCTCGACGTGGACGTGCCGCCGGCCCGGTCCCTGCGCCAGGACGGCGAGTTGTCGGTC
CGCCGGGTACCGGACACGCTGTCCGCGACGGTCGAGGCCGCGCGGCAGGCGCTGACGCAG
GAGGGGGCCATCGCGGTGATCACCGCGGACGCCGAGGCGGAGCGGACGGCCGAGGCGCTG
CGGGCGGCGGGCCTGGAGGTCGCCACCTCCGAGGACGTCGGTACGGCGGCGCGGCTCGCG
GTCCTGCCGGCGAAGGTCGTCAAGGGCCTGGAGTACGACCACGTGATCGTCGTGGAGCCG
TCGGCCATCGTGGCGGCCGAGCCGCGCGGTCTGCACCGGTTGTACGTGGCCGTGACGCGT
GCGGTGTCCCGCCTCGACGTGCTGCACGCGCACCCGCTGCCGGAGCCGCTGGCCGGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR014016 UvrD-like Helicase, ATP-binding domain (Domain) PS51198 UVRD_HELICASE_ATP_BIND
SignalP	No significant hit
TMHMM	No significant hit

Strply_00210 Strply_00230