Strply_00230 : CDS information

Location

Organism	Streptomyces lydicus
Strain	NRRL 2433 (=NBRC 13058)
Entry name	Streptolydigin
Contig	FN433113
Start / Stop / Direction	29,524 / 28,862 / - [in whole cluster] 29,524 / 28,862 / - [in contig]
Location	complement(28862..29524) [in whole cluster] complement(28862..29524) [in contig]
Type	CDS
Length	663 bp (220 aa)

Annotation

Category	4.1 transcriptional regulator
Product	TetR family transcriptional regulator
Product (GenBank)	TetR-family transcriptional regulator
Gene
Gene (GenBank)	slgR1
EC number
Keyword
Note
Note (GenBank)
Reference	ACC D1GLV1 PmId [19875077] Deciphering biosynthesis of the RNA polymerase inhibitor streptolydigin and generation of glycosylated derivatives. (Chem Biol. , 2009) [22820841] Three pathway-specific regulators control streptolydigin biosynthesis in Streptomyces lydicus. (Microbiology. , 2012) comment [PMID:19875077] streptolydigin生合成gene clusterの報告。上流ORFの挿入変異株の解析より、本ORFはstreptolydigin生産に関与する領域(PKS-NRPS system)に含まれることが示されている。 motif構成と相同性よりTetR-family transcriptional regulatorとしている。本ORFの機能解析はされていない。 [PMID:22820841] ３つのreguratory proteinの機能解析報告。 slgR1 TetR transcriptional regulator families <-this ORF slgR2 LuxR transcriptional regulator families　 slgY ATP/GTP-binding proteins of DNA and RNA helicase superfamily I slgR1 or slgR2を不活化するとstreptolydiginを産生出来ないので、SlgR1 と SlgR2はpathway-specific positive regulatorである。 slgR1 mutantは、streptolydiginの代わりに少量のstreptolydigin Cを生産した。 SlgR1 は3-methylaspartateへのglutamateの変換やputative glycoside hydrolases slgC1 and slgC2に関与するslgE1-E2の発現を制御する。
Related Reference	ACC P39885 PmId [1592820] The Streptomyces glaucescens TcmR protein represses transcription of the divergently oriented tcmR and tcmA genes by binding to an intergenic operator region. (J Bacteriol. , 1992) comment Streptomyces glaucescens HTH-type transcriptional regulator tcmR Gel retardation によって、TcmR proteinがtcmA-tcmR intergenic regionに結合し、この結合はtetracenomycin Cによって阻害されることをin vitroで示し、TcmR proteinはtcmA と tcmRの転写を阻害するrepressorとして機能することを示唆している。

Sequence

>TetR family transcriptional regulator [TetR-family transcriptional regulator]
MEERDEPMGLRERKKQRTRQAISDAAISLFLQHGFDQVSVVDVAAAAEVSKPTLFKYFPT
KEDLVVHRFADHETVAGTIVRERRSGVSPLAALHQHFVDGLARRDPITGLNDEQEVLDFH
GLVYSTPSLLTRVVQYQSRAEEALAQALAEALDAGPADVTPRLLAGQVVAAQRILAAANW
QRIVDGATADGSYPQAVADADHAFALLEWGLAGPSAARTA

>TetR family transcriptional regulator [TetR-family transcriptional regulator]
GTGGAAGAGCGAGACGAGCCGATGGGGCTCAGGGAGCGCAAGAAACAGCGGACGCGGCAG
GCGATCTCCGACGCCGCGATCTCACTGTTCCTCCAGCACGGGTTCGACCAGGTCTCGGTC
GTGGACGTGGCGGCGGCGGCCGAGGTGTCCAAGCCGACGCTGTTCAAGTACTTCCCGACC
AAGGAAGACCTGGTGGTGCACCGCTTCGCCGACCACGAGACGGTGGCGGGCACCATCGTG
CGCGAGCGCCGCTCCGGCGTCTCACCGCTCGCCGCCCTGCACCAGCACTTCGTCGACGGG
CTGGCCCGCCGCGACCCCATCACGGGCCTGAACGACGAGCAGGAGGTCCTCGACTTCCAC
GGTCTGGTGTACTCCACGCCCAGCCTGCTCACCCGCGTGGTGCAGTACCAGAGCCGCGCC
GAGGAAGCGCTGGCGCAGGCGCTGGCCGAGGCACTGGACGCCGGGCCCGCCGACGTCACG
CCCCGGCTGCTGGCCGGTCAGGTCGTCGCCGCCCAGCGCATCCTGGCCGCGGCCAACTGG
CAGCGGATCGTCGACGGCGCCACGGCGGACGGCAGTTACCCGCAGGCGGTCGCCGACGCC
GACCATGCCTTCGCGCTGCTGGAGTGGGGACTCGCCGGCCCCTCGGCAGCCCGCACCGCC
TGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001647 DNA-binding HTH domain, TetR-type (Domain) PF00440 TetR_N PR00455 HTHTETR PS50977 HTH_TETR_2 IPR009057 Homeodomain-like (Domain) SSF46689 Homeodomain_like G3DSA:1.10.10.60 Homeodomain-rel IPR015893 Tetracycline transcriptional regulator, TetR-like, C-terminal (Domain) G3DSA:1.10.357.10 TetR_C IPR023772 DNA-binding HTH domain, TetR-type, conserved site (Conserved_site) PS01081 HTH_TETR_1
SignalP	No significant hit
TMHMM	No significant hit

Strply_00220 Strply_00240