Strply_00330 Strply_00330   Strply_00350 Strply_00350

Strply_00340 : CDS information

close this sectionLocation

Organism
StrainNRRL 2433 (=NBRC 13058)
Entry nameStreptolydigin
Contig
Start / Stop / Direction77,818 / 78,480 / + [in whole cluster]
77,818 / 78,480 / + [in contig]
Location77818..78480 [in whole cluster]
77818..78480 [in contig]
TypeCDS
Length663 bp (220 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.4 hypothetical protein
Producthypothetical protein
Product (GenBank)putative integral membrane protein
Gene
Gene (GenBank)
EC number
Keyword
Note
  • It is supposed that this ORF is not involved in streptolydigin biosynthesis.
Note (GenBank)
  • ORF5
Reference
ACC
PmId
[19875077] Deciphering biosynthesis of the RNA polymerase inhibitor streptolydigin and generation of glycosylated derivatives. (Chem Biol. , 2009)
comment
streptolydigin生合成gene clusterの報告。

ORF1-6は相同性を示すSCO,SAVと同様の機能を果たすと考察している。
ORF5は、Q9K3P6(SCO1102),Q82N02(SAV1501)と相同性を示す。
いずれも hypothetical protein。


streptolydigin生合成領域を特定するために、orf2から上流および orf3から下流領域の不活化株を作製。いずれも streotolydiginの生産量が変化しなかった(data not shown)ことから、Orf2から上流およびOrf3から下流領域は streptolydiginの生合成には関与していないと考察している。
この領域は、一次代謝やhousekeepingに関与しているのだろうと推測している。

本ORFは機能解析実験はされていない。

close this sectionSequence

selected fasta
>hypothetical protein [putative integral membrane protein]
MSHGVRRALPCAALFLMLLALVVLRWGPLLSLDAAVSGALHRTAVASPGWTRVSRVLSDW
VWDPWAMRALLAVAIGLLVRRGAPLLAVWVGATALAGTALQQLVKALVGRARPVWPDPVD
SAHYAAFPSGHAMSALVAGVLLLWLLRLYGARPGWRWTARVLVVLSVVGVGCTRVFLGVH
WPSDVLGGWLLGGAVVAGSAGAYAVCARRWAPRTVPAHGA
selected fasta
>hypothetical protein [putative integral membrane protein]
ATGTCGCACGGCGTCCGCCGCGCCCTTCCCTGCGCGGCCCTGTTCCTGATGCTGCTCGCC
CTGGTCGTCCTGCGCTGGGGCCCGTTGCTGTCGCTGGACGCGGCGGTCAGCGGCGCGCTG
CACCGGACGGCCGTCGCCTCGCCGGGCTGGACCCGGGTGAGCCGGGTGCTCAGCGACTGG
GTGTGGGATCCCTGGGCGATGCGCGCGCTGCTCGCGGTCGCGATCGGGCTGCTCGTACGG
CGTGGTGCGCCGCTGCTCGCGGTCTGGGTGGGGGCCACCGCACTGGCCGGTACGGCGCTC
CAGCAGCTGGTGAAGGCGCTGGTCGGCCGGGCGCGCCCGGTCTGGCCGGATCCGGTGGAC
TCGGCGCACTACGCGGCGTTCCCGTCGGGGCATGCGATGTCGGCGCTGGTGGCGGGCGTC
CTGCTGCTGTGGCTGCTGCGGCTGTACGGGGCACGGCCCGGGTGGCGGTGGACGGCGCGG
GTGCTGGTCGTGCTCTCCGTCGTCGGCGTCGGCTGCACCCGGGTGTTTCTGGGGGTGCAC
TGGCCGTCGGACGTCCTCGGCGGGTGGCTGCTGGGCGGTGCCGTGGTGGCCGGGTCGGCC
GGGGCATACGCCGTCTGCGCGCGCCGGTGGGCGCCGCGGACCGTTCCGGCGCACGGCGCA
TGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000326 Phosphatidic acid phosphatase type 2/haloperoxidase (Family)
[83-200] 4.79999776836274e-14 SM00014
SM00014   acidPPc
[12-213] 3.1999989904635e-27 SSF48317
SSF48317   AcPase_VanPerase
[88-199] 2.3e-20 PF01569
PF01569   PAP2
IPR016118 Phosphatidic acid phosphatase/chloroperoxidase, N-terminal (Domain)
[16-213] 3.3e-31 G3DSA:1.20.144.10
G3DSA:1.20.144.10   P_Acid_Pase/Cl_peroxidase_N
SignalP
[1-31] 0.989 Signal
Eukaryota   
[1-34] 0.998 Signal
Bacteria, Gram-positive   
[1-34] 0.872 Signal
Bacteria, Gram-negative   
TMHMM
[7-29] Transmembrane (i-o) [61-78] Transmembrane (o-i) [85-104] Transmembrane (i-o) [124-146] Transmembrane (o-i) [159-181] Transmembrane (i-o) [185-207] Transmembrane (o-i)
Transmembrane 6   
Strply_00330 Strply_00330   Strply_00350 Strply_00350
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