Tatmc_00030 : CDS information

Location

Organism	Streptomyces sp.
Strain	CK4412
Entry name	Tautomycetin
Contig	DQ983361
Start / Stop / Direction	4,735 / 3,713 / - [in whole cluster] 4,735 / 3,713 / - [in contig]
Location	complement(3713..4735) [in whole cluster] complement(3713..4735) [in contig]
Type	CDS
Length	1,023 bp (340 aa)

Annotation

Category	3.1 modification hydroxylation
Product	putative hydroxylase
Product (GenBank)	TMC biosynthetic enzyme L5
Gene
Gene (GenBank)	tmcG
EC number
Keyword	dialkylmaleic anhydride moiety C-3'
Note
Note (GenBank)	gamma-butyrobetaine; 2-oxoglutarate dioxygenase; ORF L5
Reference	ACC A4KCF3 PmId [17379718] Isolation of the biosynthetic gene cluster for tautomycetin, a linear polyketide T cell-specific immunomodulator from Streptomyces sp. CK4412. (Microbiology. , 2007) comment tautomycetinの生合成遺伝子クラスターに関する文献。 tmcG: hypothetical protein (Frankia sp. CcI3), id=28% Gamma-butyrobetaine, 2-oxoglutarate dioxygenase (uncultured bacterium 578), id=13% tmcG自体の機能実験は行われていない。 tmcGに関する記載は特に無し。
Related Reference	ACC C6ZCS6 PmId [19191560] Characterization of the tautomycetin biosynthetic gene cluster from Streptomyces griseochromogenes provides new insight into dialkylmaleic anhydride biosynthesis. (J Nat Prod. , 2009) comment <Predicted hydroxylase, Streptomyces griseochromogenes, BLAST 2; id=100%> Streptomyces griseochromogenesにおけるTautomycetin Biosynthetic Gene Clusterを同定した文献。 ttnM (339 a.a): hydroxylase ttnM自体の機能実験は行われていないが、ttnM不活化変異株を作成し、Tautomycetin (TTN)生産が消失し、TTN-M1(C-3' deshydroxy-TTN)と4つの新たな化合物の蓄積が確認されている。また、この変異株にttnMを相補すると30%程度のTTNの生産が回復し、4つの新たな化合物が消失することが確認されている。
Related Reference	ACC C0SMX2 NITE Tauto_00190 PmId [19281218] Functional characterization of ttmM unveils new tautomycin analogs and insight into tautomycin biosynthesis and activity. (Org Lett. , 2009) comment Blast 3rd, id77%, 1e-148 Streptomyces spiroverticillatus_ttmM Predicted hydroxylase putative C3' hydroxylaseをコードするttmMを不活化した株では、3つの新規3'-deshydroxy TTM analogsを蓄積。よって提唱機能が支持される。また、TTMの特徴であるprotein phosphatase抑制作用にC3' OH moietyが重要であること、protein phosphatase抑制はTTM誘導細胞死を操縦する独占的機構ではないことが明らかになっている。

Sequence

>putative hydroxylase [TMC biosynthetic enzyme L5]
MMSDQPWLHRLDPAEAAEIDDALDVLLKSGKPNFAASPADFPLPTLGPRLRGIVDSIENE
PGFALVRGVPVGDKSEDEVRRLYWGLGMYIGVPMIQNNNDSSMVDIRDERRAGKLRVHKS
NQHIGFHIDSTDVVTLLCRRAASQGGTSLVVSAEAVRREMSWECPELLSALYEPLPFADV
ASPDDERPDVFLSPVFGRHEGLTTTRFYIRRVLRSQDNPDAPRLTERQLEAINKVEEIAA
RPGLVTPMQFEPGDLQMINNHLVLHGRTAFASEEPGEGRHLLRMWFSVPSSRSLPPGYEA
AWGTREGGTLRGAGPRWQLQGEFGEFQRRQAEELGVAIPA

>putative hydroxylase [TMC biosynthetic enzyme L5]
GTGATGTCCGACCAACCGTGGCTGCACCGCCTGGACCCCGCCGAGGCGGCCGAGATCGAC
GACGCGCTGGACGTGCTGCTCAAGAGCGGCAAGCCGAACTTCGCCGCGAGTCCCGCCGAC
TTCCCACTGCCCACGCTGGGGCCGAGGCTGCGCGGCATCGTCGACTCCATCGAGAACGAG
CCGGGCTTCGCCCTGGTCCGGGGAGTTCCGGTCGGCGACAAGAGCGAGGACGAGGTCCGC
CGGCTCTACTGGGGACTCGGCATGTACATCGGCGTGCCCATGATCCAGAACAACAACGAC
TCCAGCATGGTCGACATCCGCGACGAGCGCCGGGCGGGCAAGCTGCGGGTTCACAAGTCG
AATCAGCACATCGGCTTCCACATCGACTCGACCGACGTCGTCACCCTGCTGTGCCGGCGG
GCGGCCTCGCAGGGCGGCACCAGCCTCGTGGTCAGCGCCGAGGCCGTACGCCGGGAGATG
TCCTGGGAGTGCCCCGAGCTGCTGAGCGCGCTGTACGAGCCGCTGCCCTTCGCTGACGTC
GCCTCCCCCGACGACGAGCGTCCGGACGTCTTCCTGAGCCCGGTCTTCGGTCGGCACGAA
GGGCTCACCACGACCCGGTTCTACATCCGCCGGGTGCTGCGCAGCCAGGACAACCCTGAC
GCGCCGCGGCTGACCGAGCGGCAGCTCGAGGCCATCAACAAGGTCGAGGAGATCGCCGCC
CGGCCCGGCCTGGTCACGCCCATGCAGTTCGAGCCGGGCGACCTGCAGATGATCAACAAC
CACCTGGTGCTGCACGGCCGCACGGCCTTCGCCAGCGAGGAGCCCGGGGAGGGACGGCAC
CTGCTGCGGATGTGGTTCTCCGTGCCGTCGAGCCGGTCGTTGCCGCCCGGGTACGAGGCG
GCGTGGGGCACCCGCGAGGGCGGCACCCTTCGCGGCGCCGGCCCGCGCTGGCAACTGCAG
GGCGAGTTCGGCGAGTTCCAGCGCCGACAGGCGGAGGAACTGGGCGTGGCGATCCCGGCC
TGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR003819 Taurine catabolism dioxygenase TauD/TfdA (Family) PF02668 TauD
SignalP	Eukaryota
TMHMM	No significant hit

Tatmc_00020 Tatmc_00040