Tatmc_00040 : CDS information

Location

Organism	Streptomyces sp.
Strain	CK4412
Entry name	Tautomycetin
Contig	DQ983361
Start / Stop / Direction	5,867 / 4,776 / - [in whole cluster] 5,867 / 4,776 / - [in contig]
Location	complement(4776..5867) [in whole cluster] complement(4776..5867) [in contig]
Type	CDS
Length	1,092 bp (363 aa)

Annotation

Category	5.4 hypothetical protein
Product	hypothetical protein
Product (GenBank)	TMC biosynthetic enzyme L4
Gene
Gene (GenBank)	tmcF
EC number
Keyword
Note
Note (GenBank)	hypothetical protein; ORF L4
Reference	ACC A4KCF4 PmId [17379718] Isolation of the biosynthetic gene cluster for tautomycetin, a linear polyketide T cell-specific immunomodulator from Streptomyces sp. CK4412. (Microbiology. , 2007) comment tautomycetinの生合成遺伝子クラスターに関する文献。 tmcF: hypothetical protein (AMP-binding protein)(Frankia sp. EAN1pec), id=47% putative phenazine antibiotic biosynthesis protein (Erwinia carotovora subsp. atroseptica SCRI 1043), id=41% tmcF自体の機能実験は行われていない。 tmcFに関する記載は特に無し。
Related Reference	ACC C6ZCS5 PmId [19191560] Characterization of the tautomycetin biosynthetic gene cluster from Streptomyces griseochromogenes provides new insight into dialkylmaleic anhydride biosynthesis. (J Nat Prod. , 2009) comment <Putative uncharacterized protein ttnN, BLAST 1; id=100%> Streptomyces griseochromogenesにおけるTautomycetin Biosynthetic Gene Clusterを同定した文献。 ttnN (363 a.a): unknown ttnN 自体の機能実験は行われおらず、相同性からも機能の特定はできなかったとされているが、tautomycin (TTM) biosynthetic gene clusterとの比較より、TtmNに相当することからdialkylmaleic anhydride moiety biosynthesisに関与していると予想されている。

Sequence

>hypothetical protein [TMC biosynthetic enzyme L4]
MTEQWAVLDPAIRPADPEAYLRAALQWHFGEDTGSPFWLRRAKSLDFDPLTDVRTYDDLT
LFPNIVDELRDIPVEDLVPRGYGPRPPAPKVFETGGTTGAPKRVILMPDWFESSIDRMLA
GPVFAGREPGNILIATPTGPHKIGGQYDDVVRRQNTIKFTIDVDPRWVKKLIARGENDQV
KAYVEHVLDQIEHVLTTQNVGLLVITPPLLRACSARPLLVELINTKVSVVFWGGAHMTAD
ERYELQHVHFPGVRMVSRYNSAMILEGAAERAGTGTGDHVIYDPYSPVVTFRVIDPETRE
TVDYGRRGQVVMNHVSKGFFAPNNLERDSAVRVPGPEGQFGDSVADPRPVETFGGERVIE
GIY

>hypothetical protein [TMC biosynthetic enzyme L4]
ATGACCGAGCAGTGGGCGGTGCTGGACCCGGCGATCCGCCCGGCCGACCCCGAGGCCTAT
CTCAGAGCTGCGCTGCAGTGGCATTTCGGCGAGGACACCGGGTCGCCCTTCTGGCTGCGG
CGGGCGAAATCGCTGGACTTCGACCCGCTGACCGACGTGCGTACGTACGACGATCTGACG
CTGTTCCCCAACATCGTCGACGAGCTGCGCGACATTCCCGTGGAAGACCTTGTCCCGCGG
GGGTACGGCCCCCGGCCCCCGGCGCCGAAGGTCTTCGAGACCGGCGGCACCACCGGCGCG
CCCAAGCGGGTGATCCTCATGCCGGACTGGTTCGAGAGCTCCATCGACCGGATGCTCGCC
GGGCCGGTGTTCGCCGGGCGGGAGCCGGGCAACATCCTGATCGCGACGCCGACCGGCCCG
CACAAAATAGGCGGCCAGTACGACGACGTGGTGCGTCGGCAGAACACGATCAAGTTCACC
ATCGACGTCGACCCGCGCTGGGTCAAGAAGCTCATCGCCCGGGGCGAGAACGACCAGGTC
AAGGCGTACGTGGAGCACGTACTGGACCAGATCGAGCACGTCCTGACGACTCAGAACGTC
GGGCTGCTGGTGATCACGCCGCCGCTGCTGCGCGCGTGCTCCGCACGTCCGCTGCTCGTC
GAGCTCATCAACACCAAGGTGAGCGTGGTGTTCTGGGGCGGCGCGCACATGACGGCCGAC
GAGCGCTACGAGCTGCAGCACGTGCACTTCCCGGGCGTACGCATGGTGAGCCGCTACAAC
AGCGCGATGATCCTCGAGGGGGCCGCCGAGCGCGCCGGGACCGGCACCGGCGATCACGTG
ATCTACGACCCGTACAGCCCGGTGGTGACCTTCCGGGTGATCGACCCGGAGACGCGGGAG
ACGGTGGACTACGGCCGGCGCGGGCAGGTCGTGATGAACCACGTGAGCAAGGGCTTCTTC
GCGCCCAACAACCTCGAGCGGGACTCCGCCGTACGCGTGCCTGGCCCGGAGGGGCAGTTC
GGCGACTCGGTGGCCGACCCCCGGCCGGTCGAGACGTTCGGTGGTGAGCGAGTGATCGAG
GGCATCTACTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	No significant hit
SignalP	No significant hit
TMHMM	No significant hit

Tatmc_00030 Tatmc_00050