Tatmc_00050 : CDS information

Location

Organism	Streptomyces sp.
Strain	CK4412
Entry name	Tautomycetin
Contig	DQ983361
Start / Stop / Direction	6,793 / 5,864 / - [in whole cluster] 6,793 / 5,864 / - [in contig]
Location	complement(5864..6793) [in whole cluster] complement(5864..6793) [in contig]
Type	CDS
Length	930 bp (309 aa)

Annotation

Category	3.4 other modification
Product	putative lyase
Product (GenBank)	TMC biosynthetic enzyme L3
Gene
Gene (GenBank)	tmcE
EC number
Keyword	dialkylmaleic anhydride moiety
Note
Note (GenBank)	citrate lyase beta subunit; ORF L3
Reference	ACC A4KCF5 PmId [17379718] Isolation of the biosynthetic gene cluster for tautomycetin, a linear polyketide T cell-specific immunomodulator from Streptomyces sp. CK4412. (Microbiology. , 2007) comment tautomycetinの生合成遺伝子クラスターに関する文献。 tmcE: citrate lyase beta subunit (B. pseudomallei), id=44% probable citrate lyase beta subunit (B. thailandensis), id=44% tmcE自体の機能実験は行われていない。 tmcEに関する記載は特に無し。
Related Reference	ACC C6ZCS4 PmId [19191560] Characterization of the tautomycetin biosynthetic gene cluster from Streptomyces griseochromogenes provides new insight into dialkylmaleic anhydride biosynthesis. (J Nat Prod. , 2009) comment <Probable citrate lyase beta subunit, BLAST 2; id=100%> Streptomyces griseochromogenesにおけるTautomycetin Biosynthetic Gene Clusterを同定した文献。 ttnO (309 a.a): citryl CoA lyase ttnO 自体の機能実験は行われていないが、tautomycin (TTM) biosynthetic gene clusterとの比較より、TtmOに相当することからalpha-ketoglutarateのalpha-carbonyl groupとmethylmalonyl-CoAとのcondensationに関与していると予想している。
Related Reference	ACC P17725 PmId [7830578] Klebsiella pneumoniae genes for citrate lyase and citrate lyase ligase: localization, sequencing, and expression. (Mol Microbiol. , 1994) comment <Citrate lyase subunit beta, Klebsiella pneumoniae, BLAST 187; id=33%> Klebsiella pneumoniaeよりcitD(gammma-), citE(beta-) and citF(alpha-subunit of citrate lyase) の遺伝子をクローニングしてシークエンスしており、これらの遺伝子がcitC(citrate lyase ligase）、citG(機能不明）とともにcitCDEFG gene clusterを形成していることを示している。また、citCDEFGを大腸菌で発現させたところ、acetyl-CoA存在下でのみcitrate lyase活性が検出されたことが示されている。
Family/Domain	FD IPR005000 comment [IPR005000] HpcH/HpaI aldolase (Family) Function GO:0016830 carbon-carbon lyase activity

Sequence

>putative lyase [TMC biosynthetic enzyme L3]
MSTDETPRQVFRSCLYVPGHHPDRIARAYDTETDAVILDLEDAVPPPEKARAREIVAEAV
TTPAAKPTYVRVNPVSSGLCEDDIRAVAGAGLAGLRVAKVSSSEEVRLVAGVLDELGHPG
RIHVLIESAEALEHAYELATASERVTMVGLGESDLRADLNTTNEGPTMDSARARVIVAAR
AAGLPNPIQSVYAEPRDLRGLLVTSRHGRRLGFLGRMAIHPAQIPIIHDVYTPTTNEIDD
ALEVFAAADLAATESRSIAITAQGKMVGPPALARARQVLELATALDLIAEARTGTTPSPR
DTGSNGGSS

>putative lyase [TMC biosynthetic enzyme L3]
ATGAGCACCGACGAGACCCCGCGGCAGGTCTTCCGCAGCTGCCTCTACGTGCCCGGCCAT
CATCCGGACCGGATAGCCCGGGCCTACGACACCGAGACCGACGCGGTGATCCTCGACCTC
GAGGACGCCGTCCCCCCGCCGGAGAAAGCCCGGGCCCGCGAGATCGTGGCCGAGGCCGTC
ACGACCCCGGCCGCAAAGCCCACGTATGTCCGGGTGAACCCGGTGTCCTCGGGTCTGTGC
GAGGACGACATCCGGGCCGTGGCCGGCGCCGGGCTCGCGGGACTGCGGGTAGCGAAGGTC
TCCTCGTCCGAGGAGGTGCGGCTCGTCGCCGGCGTGCTCGACGAACTCGGCCATCCCGGC
CGAATCCACGTGCTCATCGAGTCGGCCGAGGCGCTGGAGCACGCATACGAGCTGGCCACC
GCGTCCGAGCGGGTGACGATGGTGGGCCTGGGCGAGTCCGACCTGCGTGCCGACCTCAAC
ACCACCAACGAGGGCCCGACCATGGACAGTGCCCGGGCCCGGGTGATCGTCGCCGCCCGC
GCCGCCGGCCTGCCCAACCCGATCCAGAGCGTCTACGCCGAGCCTCGGGACCTGCGCGGG
CTGCTGGTCACCAGCAGGCACGGCCGCCGGCTCGGGTTCCTCGGCCGGATGGCGATCCAC
CCGGCGCAGATCCCGATCATCCACGACGTCTACACCCCGACGACCAACGAGATCGACGAC
GCACTGGAGGTCTTCGCGGCCGCGGACCTCGCCGCCACGGAGAGCCGGTCGATCGCCATC
ACCGCGCAGGGCAAGATGGTCGGCCCGCCCGCCTTGGCCCGCGCCCGCCAGGTGCTGGAG
CTGGCCACCGCCCTCGATCTGATCGCGGAGGCCCGGACCGGGACCACCCCATCGCCACGT
GACACCGGGAGCAACGGAGGTTCTTCATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005000 Aldehyde-lyase domain (Domain) PF03328 HpcH_HpaI IPR011206 Citrate lyase, beta subunit (Family) PIRSF015582 Cit_lyase_B IPR015813 Pyruvate/Phosphoenolpyruvate kinase (Domain) SSF51621 Pyrv/PenolPyrv_Kinase_cat G3DSA:3.20.20.60 Pyrv/PenolPyrv_Kinase_cat
SignalP	Bacteria, Gram-positive
TMHMM	No significant hit

Tatmc_00040 Tatmc_00060