Tatmc_00060 Tatmc_00060   Tatmc_00080 Tatmc_00080

Tatmc_00070 : CDS information

close this sectionLocation

Organism
StrainCK4412
Entry nameTautomycetin
Contig
Start / Stop / Direction9,456 / 7,960 / - [in whole cluster]
9,456 / 7,960 / - [in contig]
Locationcomplement(7960..9456) [in whole cluster]
complement(7960..9456) [in contig]
TypeCDS
Length1,497 bp (498 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.4 other modification
Productputative esterase
Product (GenBank)TMC biosynthetic enzyme L1
Gene
Gene (GenBank)tmcC
EC number3.1.1.-
Keyword
  • dialkylmaleic anhydride moiety
Note
Note (GenBank)
  • type B carboxylesterase; ORF L1
Reference
ACC
PmId
[17379718] Isolation of the biosynthetic gene cluster for tautomycetin, a linear polyketide T cell-specific immunomodulator from Streptomyces sp. CK4412. (Microbiology. , 2007)
comment
tautomycetinの生合成遺伝子クラスターに関する文献。

tmcC:
type B carboxyesterase (Bacillus sp. BP-7), id=28%
thermostable carboxylesterase (Geobacillus kaustophilus), id=26%

tmcC 自体の機能実験は行われていないが、相同性よりcyclic C8 dialkylmaleic anhydride moietyをlinear polyketide moietyにlinkする機能を持つと予想している。
Related Reference
ACC
C6ZCS2
PmId
[19191560] Characterization of the tautomycetin biosynthetic gene cluster from Streptomyces griseochromogenes provides new insight into dialkylmaleic anhydride biosynthesis. (J Nat Prod. , 2009)
comment
<Putative type B carboxylesterase, BLAST 2; id=100%>

Streptomyces griseochromogenesにおけるTautomycetin Biosynthetic Gene Clusterを同定した文献。

ttnK (465 a.a): esterase

ttnK自体の機能実験は行われていないが、相同性よりesteraseであるとし、dialkylmaleic anhydride moietyとpolyketide chainをcoupleする機能を持つと予想している。
ACC
C0SMW5
NITE
Tauto_00120
PmId
[18708355] Characterization of the tautomycin biosynthetic gene cluster from Streptomyces spiroverticillatus unveiling new insights into dialkylmaleic anhydride and polyketide biosynthesis. (J Biol Chem. , 2008)
comment
<Putative type B carboxylesterase, Streptomyces spiroverticillatus, BLAST 3; id=71%>

tautomycin (TTM) biosynthetic gene clusterについての文献。

ttmK(456a.a.): esterase

ttmKを不活化すると、TTMの生産が見られないことを確認している。機能としては、dialkylmaleic anhydride moietyをpolyketide chainにcouplingするものと予想されている。
ACC
Q9L378
PmId
[11178722] Cloning and characterization of a bacterial cell-bound type B carboxylesterase from Bacillus sp. BP-7. (Curr Microbiol. , 2001)
comment
<Type B carboxylesterase, Bacillus sp. BP-7, BLAST 6; id=37%>

carboxylesteraseと相同性のあるORFを含んだ領域をクローニングし、短鎖脂肪酸のesters選択的に機能するesteraseであることを確認している。

close this sectionSequence

selected fasta
>putative esterase [TMC biosynthetic enzyme L1]
MKPPALVVPGPVPPWNYRRGSRPTRPRLRELTAMGDHFDRDLVVETSSGTVRGFLDRGTP
NWRGVPYGRVEQRFHPPVPALPGEPVDGRHWGPICWQLPIVAGRSPSSVFPDAAESEDCL
NLNVWSPGPDPGARRPVLVWLHFGSRAYGAGSTPMIDVWHFAARHNAVVVTPNYRLGPWG
WLYLGALDPEFEDSANLALLDQILMLRWVKDNIAAFGGDPDNVTVFGMSSGASDLGALLG
APAARGLFHKAASYSGAQAPMLEKDATEFAEEFLAAAGPLAATAAELAALPNVGLRTVHR
RLLKQSVSRAGGPIIIRYGPFVDGSVLPKAPLDALADGLMAGVALLVSNVAEEAGVWDAW
GAVDHAYSRVFAGSDQSVSHEEKVEQLSEYRWVGPARKLIAAHHQGGGTGWMQKFDYAPT
TNWQTALSYPEVARRPVHGADVASLFLDPEGEVGSEDDRAVAALDQAALLALARDGRAPW
TPWSPDNQTPHRIAAAAH
selected fasta
>putative esterase [TMC biosynthetic enzyme L1]
GTGAAACCACCAGCATTAGTGGTTCCGGGGCCGGTGCCGCCCTGGAACTATCGACGCGGG
TCTCGCCCCACCCGCCCTCGACTCCGGGAGCTGACAGCCATGGGTGACCATTTTGATCGC
GACTTGGTGGTGGAGACGTCGTCCGGCACGGTCCGGGGTTTCCTGGACCGCGGGACACCG
AACTGGCGGGGCGTGCCCTACGGCCGGGTCGAACAGCGTTTCCACCCGCCGGTGCCCGCG
TTGCCGGGCGAGCCGGTCGACGGCCGCCACTGGGGGCCGATCTGCTGGCAGCTGCCGATC
GTGGCCGGCCGGAGCCCGTCCTCGGTCTTCCCCGACGCCGCCGAGTCCGAGGACTGCCTC
AACCTGAACGTCTGGTCGCCCGGTCCGGACCCGGGCGCCCGGCGGCCGGTGCTGGTGTGG
CTGCACTTCGGCAGCCGGGCCTACGGTGCGGGGAGCACCCCGATGATCGACGTGTGGCAC
TTCGCCGCCCGGCACAACGCCGTCGTCGTGACCCCGAACTATCGGCTCGGCCCGTGGGGC
TGGCTCTATCTGGGCGCGCTGGACCCGGAGTTCGAGGACAGCGCCAACCTTGCCCTGCTC
GACCAGATCCTCATGCTGCGCTGGGTCAAGGACAACATCGCGGCCTTCGGCGGTGACCCG
GACAACGTCACCGTCTTCGGCATGTCTTCAGGCGCCTCCGACCTGGGCGCGCTGCTGGGC
GCTCCGGCGGCCCGAGGGCTGTTCCACAAGGCCGCCTCCTACAGCGGGGCGCAGGCGCCG
ATGCTGGAGAAGGACGCCACGGAGTTCGCCGAGGAGTTCCTGGCGGCCGCGGGCCCGCTG
GCGGCCACGGCGGCCGAGCTGGCCGCCCTGCCCAACGTCGGCCTGCGGACCGTGCACCGG
CGGCTGCTGAAGCAGAGCGTCTCGCGCGCCGGAGGCCCGATCATCATCCGGTACGGGCCC
TTCGTCGACGGCTCCGTCCTGCCCAAGGCGCCCCTCGACGCGCTGGCCGACGGTCTCATG
GCCGGAGTCGCCCTGCTGGTGTCCAACGTCGCTGAGGAGGCGGGTGTGTGGGACGCCTGG
GGTGCGGTCGACCACGCCTACTCGCGCGTCTTCGCCGGCAGTGACCAGTCGGTGTCGCAC
GAGGAGAAGGTGGAACAGCTGTCCGAGTACCGCTGGGTCGGGCCGGCCCGGAAGCTGATC
GCCGCCCATCACCAGGGCGGCGGGACCGGCTGGATGCAGAAGTTCGACTACGCGCCGACG
ACCAACTGGCAGACCGCGCTCTCCTATCCCGAGGTGGCAAGGCGGCCGGTGCACGGCGCT
GACGTGGCGTCGCTGTTCCTCGACCCCGAGGGCGAGGTCGGCTCCGAGGACGACCGCGCG
GTCGCCGCGCTGGACCAGGCCGCTCTGCTCGCCTTGGCCCGCGACGGTCGCGCGCCCTGG
ACGCCGTGGTCACCGGACAACCAGACCCCGCACCGGATAGCCGCCGCGGCACACTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002018 Carboxylesterase, type B (Domain)
[40-357] 1.10000000000001e-66 PF00135
PF00135   COesterase
IPR019826 Carboxylesterase type B, active site (Active_site)
[216-231] PS00122
PS00122   CARBOXYLESTERASE_B_1
SignalP No significant hit
TMHMM No significant hit
Tatmc_00060 Tatmc_00060   Tatmc_00080 Tatmc_00080
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