Tetrn_00180 Tetrn_00180   Tetrn_00200 Tetrn_00200

Tetrn_00190 : CDS information

close this sectionLocation

Organism
StrainNRRL 11266
Entry nameTetronomycin
Contig
Start / Stop / Direction83,903 / 84,145 / + [in whole cluster]
83,903 / 84,145 / + [in contig]
Location83903..84145 [in whole cluster]
83903..84145 [in contig]
TypeCDS
Length243 bp (80 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.5 electron carrier
Productputative ferredoxin
Product (GenBank)putative ferredoxin
Gene
Gene (GenBank)tmn14a
EC number
Keyword
  • C-28 hydroxylate
Note
Note (GenBank)
Reference
ACC
PmId
[18404760] Analysis of the tetronomycin gene cluster: insights into the biosynthesis of a polyether tetronate antibiotic. (Chembiochem. , 2008)
comment
Streptomyces sp. NRRL 11266におけるtetronomycin (TMN) gene clusterに関する文献。

tmn14a(80 aa): ferredoxin

tmn14aについての機能実験等は無し。相同性より機能名を付けている模様。TMN生合成の最終段階で、cognateのtmn14(cytochrome P450)と共にC-28特異的にhydroxylationする機能が予想されている。
Related Reference
ACC
P18325
PmId
[1846297] Ferredoxins from two sulfonylurea herbicide monooxygenase systems in Streptomyces griseolus. (Biochemistry. , 1991)
comment
<Ferredoxin-2(subB), Streptomyces griseolus, BLAST 60; id=36%>

sulfonylurea herbicideが誘発されたStreptomyces griseolusの可溶性タンパク質フラクションより、Fd-1 と Fd-2の二種類のフェレドキシンを精製し、どちらも、aerobic mixture of NADPH、spinach ferredoxin:NADP oxidoreductase、精製したcytochrome P-450SU1とherbicide基質にsulfonylurea monooxygenase活性を復元できることが示されている。また、cytochromes P-450, P-450SU1 (CYP105A1) と P-450SU2 (CYP105B1)は、それぞれFd-1 と Fd-2の近傍に位置していることが示されている。

close this sectionSequence

selected fasta
>putative ferredoxin [putative ferredoxin]
MRDSSMQVETEIERCVASGMCTLLAPEVFDQDEVTGQVVVLVKRPAEEQWEAVDEAVRSC
PGQVVRFEEAAEPAGAAEAP
selected fasta
>putative ferredoxin [putative ferredoxin]
ATGCGGGATTCCTCCATGCAGGTCGAGACCGAGATCGAGCGGTGCGTGGCATCTGGCATG
TGCACGCTCCTGGCGCCGGAGGTCTTCGACCAGGATGAGGTGACAGGTCAGGTCGTGGTG
CTGGTGAAGCGCCCGGCCGAGGAGCAGTGGGAGGCGGTGGACGAGGCCGTGCGCTCCTGC
CCGGGCCAGGTCGTCCGGTTCGAGGAGGCGGCCGAGCCGGCCGGGGCGGCCGAGGCGCCT
TAA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0 		No significant hit
SignalP No significant hit
TMHMM No significant hit
Tetrn_00180 Tetrn_00180   Tetrn_00200 Tetrn_00200
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