Tetrn_00200 : CDS information

Location

Organism	Streptomyces sp.
Strain	NRRL 11266
Entry name	Tetronomycin
Contig	AB193609
Start / Stop / Direction	84,250 / 85,452 / + [in whole cluster] 84,250 / 85,452 / + [in contig]
Location	84250..85452 [in whole cluster] 84250..85452 [in contig]
Type	CDS
Length	1,203 bp (400 aa)

Annotation

Category	3.3 modification reduction
Product	putative cytochrome P450
Product (GenBank)	putative cytochrome P450-like protein
Gene
Gene (GenBank)	tmn14
EC number
Keyword	C-28 hydroxylate
Note
Note (GenBank)
Reference	ACC Q1MX69 PmId [18404760] Analysis of the tetronomycin gene cluster: insights into the biosynthesis of a polyether tetronate antibiotic. (Chembiochem. , 2008) comment Streptomyces sp. NRRL 11266におけるtetronomycin (TMN) gene clusterに関する文献。 tmn14(400 aa): P450 monooxygenase tmn14についての機能実験等は無し。相同性より機能名を付けている模様。TMN生合成の最終段階で、cognateのtmn14a(ferredoxin)と共にC-28特異的にhydroxylationする機能が予想されている。
Related Reference	ACC A6YRR3 PmId [19376895] Characterization of P450 FcpC, the enzyme responsible for bioconversion of diosgenone to isonuatigenone in Streptomyces virginiae IBL-14. (Appl Environ Microbiol. , 2009) comment Blast 25th, id51%, 2e-99 Streptomyces virginiae Cytochrome P450 monooxygenase 105 cytochrome P450 monooxygenase, FcpCを同定した文献。 FcpCがdiosgenoneのC-25のhydroxylationを担い、isonuatigenoneに変換する機能を持つことがin vitroで示されている。この論文で同定されたfcpC(1191nt) and fcpD(243nt)遺伝子配列のGenBank accession numberはEF646279であると記載あり。よってこのentry=FcpCで合っている。

Sequence

>putative cytochrome P450 [putative cytochrome P450-like protein]
MSDTLSTPPRRLPTERPRPLDPPEELGRLRAEEPVSPLLYPDGHVGWLVTSHAAARKVLS
DQRFSARGDIKKVPFELPSGGRPWEKVPPGFFMHMDPPDHTRYRRLLTGQFTVRRMKALE
PRIEQITEEHLDEMERQGPPTDLVPSFALPIPSLAICELLGVPYGERQRFQDHSTTVMRI
GSTGEEVAAAFAAIYGLIHELVQLKHKEPEDDLLSGLIATGELDDAELTGIGMLLLMAGH
ETTASMLSLGTYALLRHPGEVKRLREDPKLFANAVEELMRYLTIAQFGVPRTALEDVELE
GKLIKAGDGVTVSLAAANRDPGRFEDADGFDVGRSTSGHMAFSYGIHQCLGQQLARVEMR
IAYSALFRRFPELRLAVDADEVPLRAEATMYAASSLPVAW

>putative cytochrome P450 [putative cytochrome P450-like protein]
ATGTCGGACACGCTGTCCACGCCGCCCCGGCGGTTGCCGACCGAGCGCCCGCGCCCGCTG
GACCCGCCGGAAGAGCTGGGCCGGCTGCGTGCCGAGGAGCCGGTCAGCCCGCTGCTGTAC
CCGGACGGGCACGTGGGCTGGCTGGTGACGAGCCACGCCGCGGCCCGGAAGGTGCTCAGC
GACCAGCGGTTCAGCGCCCGGGGCGACATCAAGAAGGTGCCGTTCGAGCTGCCCTCCGGC
GGTCGGCCGTGGGAGAAGGTGCCGCCCGGCTTCTTCATGCACATGGACCCGCCGGACCAC
ACCCGCTACCGGCGGCTGCTCACCGGCCAGTTCACGGTGCGGCGGATGAAGGCGCTGGAG
CCGCGGATCGAGCAGATCACCGAGGAGCACCTGGACGAGATGGAGCGGCAGGGGCCGCCC
ACGGACCTGGTGCCCTCGTTCGCGCTGCCGATCCCCTCGCTGGCCATCTGCGAGCTGCTG
GGCGTCCCGTACGGCGAGCGCCAGCGCTTCCAGGACCACTCGACGACCGTGATGCGCATC
GGCTCGACCGGCGAGGAGGTGGCGGCGGCCTTCGCCGCGATCTACGGGCTGATCCACGAG
CTGGTGCAGCTCAAGCACAAGGAGCCCGAGGACGACCTGCTCAGCGGGCTGATCGCCACC
GGCGAGCTGGACGACGCGGAGCTGACCGGCATCGGGATGCTGCTGCTGATGGCGGGGCAC
GAGACGACCGCCAGCATGCTGTCGCTCGGCACGTACGCGCTGCTGCGCCACCCCGGCGAG
GTCAAGCGGCTGCGCGAGGACCCGAAGCTGTTCGCCAACGCGGTCGAGGAGCTGATGCGG
TATCTGACGATCGCCCAGTTCGGCGTGCCGCGTACCGCGCTGGAGGACGTCGAGCTGGAG
GGCAAGCTCATCAAGGCCGGCGACGGCGTGACGGTGTCCCTGGCGGCGGCCAACCGCGAC
CCGGGGCGCTTCGAGGACGCGGACGGCTTCGACGTCGGCCGCTCCACCTCCGGGCACATG
GCGTTCAGCTACGGCATCCACCAGTGCCTGGGCCAGCAGCTGGCCCGGGTGGAGATGCGG
ATCGCGTACTCGGCGCTCTTCCGCCGGTTCCCCGAGCTGCGCCTCGCGGTCGACGCGGAC
GAGGTGCCGCTGCGCGCGGAGGCGACGATGTACGCCGCGAGTTCCCTCCCGGTGGCCTGG
TGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001128 Cytochrome P450 (Family) PF00067 p450 SSF48264 Cytochrome_P450 PR00385 P450 G3DSA:1.10.630.10 Cyt_P450 IPR002397 Cytochrome P450, B-class (Family) PR00359 BP450 IPR017972 Cytochrome P450, conserved site (Conserved_site) PS00086 CYTOCHROME_P450
SignalP	No significant hit
TMHMM	No significant hit

Tetrn_00190 Tetrn_00210