Tetrn_00210 : CDS information

Location

Organism	Streptomyces sp.
Strain	NRRL 11266
Entry name	Tetronomycin
Contig	AB193609
Start / Stop / Direction	85,457 / 86,497 / + [in whole cluster] 85,457 / 86,497 / + [in contig]
Location	85457..86497 [in whole cluster] 85457..86497 [in contig]
Type	CDS
Length	1,041 bp (346 aa)

Annotation

Category	2.1 modification addition of extender units
Product	putative acyltransferase
Product (GenBank)	putative 3-oxoacyl-(acyl-carrier-protein) synthase III
Gene
Gene (GenBank)	tmn15
EC number
Keyword	glyceroyl-CoA
Note
Note (GenBank)
Reference	ACC Q1MX68 PmId [18404760] Analysis of the tetronomycin gene cluster: insights into the biosynthesis of a polyether tetronate antibiotic. (Chembiochem. , 2008) comment Streptomyces sp. NRRL 11266におけるtetronomycin (TMN) gene clusterに関する文献。 tmn15(346 aa): 3-oxoacyl-ACP synthase III tmn15の不活化変異株ではTMN生産が消失することが示されている。また、相同性より3-oxoacyl-ACP synthase III と予測している。
Related Reference	ACC P68795 PmId [11959561] Purification, characterization, and identification of novel inhibitors of the beta-ketoacyl-acyl carrier protein synthase III (FabH) from Staphylococcus aureus. (Antimicrob Agents Chemother. , 2002) comment <3-oxoacyl-[acyl-carrier-protein] synthase 3(fabH), Staphylococcus aureus, BLAST 272, id=27%> fabH の遺伝子をクローニングしてE. coliでN末にHis-tagを付けて過剰発現させて精製し、諸性質を調べている。FabHはhomodimeric structureで、malonyl-ACPに対してのKm値(1.76±0.40μM)と共に、acetyl-CoA, butyryl-CoA, isobutyryl-CoAに対して活性を持つことが示されている。

Sequence

>putative acyltransferase [putative 3-oxoacyl-(acyl-carrier-protein) synthase III]
MLVLNPEQDLRVAGLGTASPGPQIDNATLGRRMGLGPMWEQWAEAFVGTKYRHLAVDLET
GEPRFSLADLGETAGRRALAAAGMEPSDVDVIVMGTAMPDMLMPTTVNVVADRLGIDQVP
TYQLQSGCSGAVQALSVAAGLLLSGSHRTALVLGGDVSAKLVDPDMDAAALPPAQLVNLM
LFGDGAGALVLTAEPSPGPALRAVFTKLVGLGRQPGQTLDWYGASRRFDGPPVSEDYKAI
EAAVPEMAAESAEELLGGLDWKADELAYVLPPQLSGRMTALIRERMGAPEAQEVSCVDET
GNNGNALVFFQLARLLPKLTAGDRALGVAIESSKWIKSGFALEATA

>putative acyltransferase [putative 3-oxoacyl-(acyl-carrier-protein) synthase III]
GTGCTCGTTCTGAACCCGGAGCAGGACCTGCGGGTCGCCGGCCTCGGCACGGCGTCGCCG
GGACCGCAGATCGACAACGCGACGCTGGGCCGCCGCATGGGCCTCGGCCCGATGTGGGAG
CAGTGGGCGGAGGCGTTCGTCGGCACCAAGTACCGCCATCTCGCGGTGGATCTGGAGACC
GGCGAGCCGCGGTTCTCGCTCGCCGACCTCGGCGAGACCGCCGGCCGGCGGGCGCTGGCC
GCGGCGGGCATGGAGCCGTCGGACGTCGACGTGATCGTGATGGGCACGGCGATGCCCGAC
ATGCTGATGCCGACGACGGTGAACGTGGTCGCGGACCGGCTGGGCATCGACCAGGTGCCC
ACGTACCAGCTGCAGTCCGGGTGCTCGGGCGCGGTGCAGGCGCTGTCGGTGGCGGCGGGG
CTGCTGCTGTCCGGCAGCCACCGCACGGCACTGGTGCTCGGCGGCGACGTCTCGGCGAAG
CTCGTCGACCCGGACATGGACGCCGCCGCGCTGCCGCCCGCGCAGCTGGTGAACCTCATG
CTGTTCGGCGACGGCGCCGGCGCGCTGGTGCTGACGGCGGAGCCGTCGCCCGGGCCGGCG
CTGCGGGCGGTCTTCACCAAGCTCGTGGGGCTGGGCCGTCAGCCCGGGCAGACGCTCGAC
TGGTACGGCGCGAGCCGCCGGTTCGACGGCCCGCCGGTGAGCGAGGACTACAAGGCGATC
GAGGCGGCCGTGCCGGAGATGGCCGCCGAGTCGGCCGAGGAACTCCTAGGCGGGCTGGAC
TGGAAGGCGGACGAGCTGGCGTACGTGCTGCCGCCGCAGCTCTCGGGCCGGATGACGGCG
CTGATCCGGGAGCGGATGGGTGCGCCCGAGGCCCAGGAGGTCTCCTGCGTCGACGAGACC
GGCAACAACGGCAACGCGCTGGTCTTCTTCCAGCTGGCGCGGCTGCTGCCGAAGCTCACC
GCGGGCGATCGCGCGCTGGGGGTCGCCATCGAGTCGAGCAAGTGGATCAAGTCGGGCTTC
GCGCTGGAGGCGACCGCCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR013747 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C-terminal (Domain) PF08541 ACP_syn_III_C IPR013751 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III (Domain) PF08545 ACP_syn_III IPR016038 Thiolase-like, subgroup (Domain) G3DSA:3.40.47.10 Thiolase-like_subgr IPR016039 Thiolase-like (Domain) SSF53901 Thiolase-like
SignalP	No significant hit
TMHMM	No significant hit

Tetrn_00200 Tetrn_00220