Virg_00260 Virg_00260   Virg_00280 Virg_00280

Virg_00270 : CDS information

close this sectionLocation

Organism
StrainMAFF 10-06014
Entry nameVirginiamycin
Contig
Start / Stop / Direction94,747 / 92,714 / - [in whole cluster]
1 / 2,034 / + [in contig]
Locationcomplement(92714..94747) [in whole cluster]
1..2034 [in contig]
TypeCDS
Length2,034 bp (677 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.2 modification addition of starter unit
Productputative branched-chain alpha-keto acid dehydrogenase E1 component
Product (GenBank)branched-chain alpha-keto acid decarboxylase
GenebkdA
bkdA/bkdB
Gene (GenBank)bkdA
EC number1.2.4.4
Keyword
  • virginiamycin M
  • isobutyryl-CoA
Note
Note (GenBank)
Reference
ACC
PmId
[17375285] Identification of the bkdAB gene cluster, a plausible source of the starter-unit for virginiamycin M production in Streptomyces virginiae. (Arch Microbiol. , 2007)
comment
branched-chain alpha-keto acid dehydrogenase (BCDH) complex bkdABの機能解析報告。

アミノ酸配列の相同性と酵素活性により、BkdAはE1 alpha,beta subunitのfusionタイプであることが示された。

bkdA欠損株を作製。
virginiamycin Mの生産能を欠いていたが、virginiamycin Sの生産には影響がなかった。
相補実験により、virginiamycin Mの生産能を回復したことを確認済み。
bkd geneは細胞の生育や形態分化には関与していなかった。
宿主大腸菌でのbkdA過剰発現株作製し触媒活性を測定、bkdAがalpha beta subunitの融合形成をコードする事を確認。
以上より、bkdAB clusterは、virginiamycin Mの生産に必須であることを示した。

Virginiamycin M産生におけるスターターユニットisobutyryl-CoAの提供をプライマーユニットとして経由しているのではないかと予測している。

close this sectionSequence

selected fasta
>putative branched-chain alpha-keto acid dehydrogenase E1 component [branched-chain alpha-keto acid decarboxylase]
MPDAAPDAKKKKAKSSENVLSRAGGTGDTIPRDRRAAFLEQMMLSRECDRRSGIVLRQGQ
AWFHISSAGHEALAALCELLEPEDLIFPHYRDRTLMLARGMDAEGQARDLMAKGGSHSAG
RNMSSHFSHRPGNVFSLASPTGSQCLPAAGAAWASVLSGERKVVVCSIGDASTRQGEFFE
ALAFAVERKLPVVFLVSDNRYGISTPTDGLSPQRLGLMPDAITKVVDGSDPDAVHAAAAA
VLPDVRAGRGPAVLWCRLDRLDSHTSSDDQRLYRTKDELAAMRDPVALFTDRLEAEGTIV
PGWADQVRARLADDVEEVFDRVAGEPSADPGEVMDHLFAAADEQPTAPADTRPCGGTMVE
AVNRALRTGLENDPTLVLFGEDIEDPKGGVFGFTKGLGTLAGPRMTNSPLAEATIVGAAV
GLAAAGMRPVVELQFVDFAGPAWNQIASQLTTLRWRTASAWRCPVVIYAPWGGYLPGGGI
WHSQSNESLFTHLPGLRVVVPSTPEDTEAVFLESFASPDPTLILLPKHLMRRQHPPQPGP
APARGARLLRTGADVTIATWGNGTELATEAADRLAAEGVGTEVIDLRWLTPVDREAVAAS
VRRTGRLVVVQEDNRTSSFGATVLADLLGSDDEFYSLLAPPRLVSRRDVHIPFHPDLESA
VLPAADDVVAAIRSVLA
selected fasta
>putative branched-chain alpha-keto acid dehydrogenase E1 component [branched-chain alpha-keto acid decarboxylase]
GTGCCCGACGCTGCTCCGGATGCGAAGAAGAAAAAGGCCAAGAGTTCCGAGAACGTGCTG
TCGCGCGCCGGCGGAACCGGGGACACGATTCCCCGGGACCGGCGAGCGGCCTTTCTGGAG
CAGATGATGCTCTCGCGCGAATGCGACCGGCGTTCCGGGATCGTTCTGCGGCAGGGCCAG
GCGTGGTTCCACATCTCATCGGCCGGCCACGAAGCCCTCGCAGCCCTGTGTGAACTGCTG
GAACCCGAGGACCTGATCTTCCCCCACTACCGCGACCGCACCCTGATGCTGGCGCGCGGA
ATGGATGCCGAAGGACAGGCCCGTGACCTCATGGCCAAGGGCGGATCGCATTCCGCCGGA
CGTAATATGAGTTCGCACTTCAGCCACCGCCCGGGAAATGTCTTCTCCCTCGCCAGCCCG
ACCGGAAGCCAGTGCCTGCCGGCGGCCGGCGCCGCGTGGGCGTCCGTACTGAGCGGCGAG
AGGAAGGTCGTCGTCTGCTCCATCGGCGACGCCTCGACGCGACAGGGTGAATTCTTCGAA
GCGCTCGCCTTCGCGGTCGAACGGAAACTCCCGGTCGTCTTCCTCGTCTCCGACAACCGC
TACGGAATCAGCACCCCGACCGACGGCCTCTCGCCGCAGCGGCTCGGCCTGATGCCGGAC
GCGATCACCAAGGTCGTCGACGGGTCGGACCCGGACGCCGTGCACGCCGCCGCGGCCGCC
GTGCTGCCGGACGTCCGCGCCGGCCGGGGCCCGGCCGTGCTGTGGTGCCGGCTGGACAGG
CTCGACTCGCACACATCCTCCGACGACCAGCGGCTCTACCGCACCAAGGACGAACTCGCC
GCCATGCGCGACCCGGTGGCCCTCTTCACCGACCGGCTGGAAGCCGAGGGGACGATCGTC
CCCGGCTGGGCGGACCAGGTCCGGGCCCGGCTCGCCGACGACGTCGAGGAGGTCTTCGAC
CGTGTGGCCGGAGAACCCTCCGCCGACCCCGGCGAGGTGATGGACCACCTGTTCGCCGCG
GCGGACGAGCAGCCGACCGCCCCCGCCGACACCCGCCCCTGCGGCGGAACGATGGTCGAG
GCGGTGAACCGGGCCCTGCGCACCGGGCTGGAGAACGACCCGACGCTGGTGCTGTTCGGC
GAGGACATCGAGGACCCGAAGGGCGGCGTCTTCGGATTCACCAAGGGCCTCGGCACCCTC
GCCGGCCCCCGGATGACCAACTCCCCGCTCGCCGAGGCCACCATCGTCGGCGCCGCCGTC
GGGCTCGCTGCGGCCGGCATGCGGCCGGTGGTCGAGCTCCAGTTCGTCGACTTCGCCGGA
CCGGCCTGGAACCAGATCGCCTCCCAGCTGACCACCCTGCGCTGGCGGACGGCCTCCGCC
TGGCGCTGCCCCGTCGTGATCTACGCACCCTGGGGCGGCTACCTGCCGGGCGGCGGAATC
TGGCACAGCCAGAGCAACGAGAGCCTGTTCACCCACCTGCCCGGGCTGCGGGTCGTGGTG
CCCTCCACCCCCGAGGACACCGAGGCGGTCTTCCTGGAGTCCTTCGCATCCCCCGACCCG
ACGCTGATCCTGCTCCCCAAGCACCTGATGCGCCGCCAGCACCCGCCGCAGCCCGGACCG
GCACCCGCCCGGGGCGCCCGGCTGCTGCGTACCGGAGCCGACGTCACCATCGCCACCTGG
GGCAATGGCACCGAACTGGCCACGGAGGCGGCGGACCGGCTGGCCGCCGAGGGCGTCGGC
ACCGAGGTGATCGACCTGCGCTGGTTGACCCCGGTCGACCGAGAGGCGGTCGCCGCCTCC
GTCCGGCGGACCGGCCGTCTGGTGGTGGTCCAGGAGGACAACCGCACCAGCAGTTTCGGC
GCCACCGTCCTGGCCGACCTCCTCGGCAGTGATGACGAGTTCTACTCGCTGCTCGCGCCG
CCCCGGCTCGTATCCCGCCGGGACGTCCACATCCCCTTCCACCCCGACCTGGAGAGCGCC
GTGCTGCCCGCGGCGGACGACGTGGTCGCGGCCATCCGATCGGTGCTGGCATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001017 Dehydrogenase, E1 component (Domain)
[42-328] 3.40000000000004e-40 PF00676
PF00676   E1_dh
IPR005475 Transketolase-like, pyrimidine-binding domain (Domain)
[357-531] 3e-31 PF02779
PF02779   Transket_pyr
[356-533] 1.20000117458134e-49 SM00861
SM00861   Transket_pyr
IPR005476 Transketolase, C-terminal (Domain)
[546-665] 3.2e-25 PF02780
PF02780   Transketolase_C
IPR009014 Transketolase, C-terminal/Pyruvate-ferredoxin oxidoreductase, domain II (Domain)
[540-677] 1.29999924468179e-31 SSF52922
SSF52922   Transketo_C_like
IPR015941 Transketolase-like, C-terminal (Domain)
[548-673] 2.70000000000002e-34 G3DSA:3.40.50.920
G3DSA:3.40.50.920   Transketo_C_like
SignalP No significant hit
TMHMM No significant hit
Virg_00260 Virg_00260   Virg_00280 Virg_00280
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